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![Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may potentially be the post-translationally modified (glycosylated, palmitoylated or lipidated) form of this protein.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Western-Blot-NBP1-03363-img0004.jpg "Western Blot: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Detection of EDG2 in A549 whole cell extracts using NBP1-03363. The band at ~41 kDa position represents the target protein EDG2, whereas, the band at ~55kDa may potentially be the post-translationally modified (glycosylated, palmitoylated or lipidated) form of this protein.")
![Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EDG2/LPAR1/LPA1 (NBP1-03363) at a 1:100 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-03363-img0005.jpg)
![Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human prostate cancer using LPAR1 antibody at 1:100 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was obseved in the scattered ducts. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Immunohistochemistry-Paraffin-NBP1-03363-img0006.jpg)
![Simple Western: LPAR1/LPA1/EDG-2 Antibody [NBP1-03363] - Simple Western lane view shows a specific band for EDG2 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Simple-Western-NBP1-03363-img0002.jpg)
![Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Skin, Melanoma](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Immunohistochemistry-Paraffin-NLS212-img0002.jpg "Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Skin, Melanoma")
![Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS212] - Brain, Glioblastoma](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Immunohistochemistry-Paraffin-NLS212-img0001.jpg)
![Western Blot: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EDG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EDG2.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-1G6-Western-Blot-NBP2-01354-img0004.jpg "Western Blot: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EDG2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EDG2.")
![Immunocytochemistry/Immunofluorescence: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY EDG2.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-1G6-Immunocytochemistry-Immunofluorescence-NBP2-01354-img0005.jpg)
![Flow Cytometry: LPAR1/LPA1/EDG-2 Antibody (1G6) [NBP2-01354] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EDG2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-1G6-Flow-Cytometry-NBP2-01354-img0006.jpg)
![Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS211] - Brain, Pituitary](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Immunohistochemistry-Paraffin-NLS211-img0002.jpg "Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS211] - Brain, Pituitary")
![Immunohistochemistry-Paraffin: LPAR1/LPA1/EDG-2 Antibody [NLS211] - Lung, Asthma, Vessel](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-Immunohistochemistry-Paraffin-NLS211-img0001.jpg)
![Western Blot: LPAR1/LPA1/EDG-2 Antibody (3U1Q0) [NBP3-15851] - Analysis of extracts of various cell lines, using LPAR1/LPA1/EDG-2 Rabbit mAb (NBP3-15851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-3U1Q0-Western-Blot-NBP3-15851-img0001.jpg "Western Blot: LPAR1/LPA1/EDG-2 Antibody (3U1Q0) [NBP3-15851] - Analysis of extracts of various cell lines, using LPAR1/LPA1/EDG-2 Rabbit mAb (NBP3-15851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: LPAR1/LPA1/EDG-2 Antibody (SR1651) [NBP3-21799] - Western blot analysis using NBP3-21799 in A375 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21799_rabbit-lpar1-lpa1-edg-2-mab-sr1651-672023106274.jpg "Western Blot: LPAR1/LPA1/EDG-2 Antibody (SR1651) [NBP3-21799] - Western blot analysis using NBP3-21799 in A375 cell lysate.")
![SDS-Page: Recombinant Human LPAR1/LPA1/EDG-2 Protein [H00001902-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-LPAR1-LPA1-EDG-2-Protein-SDS-Page-H00001902-Q01-img0002.jpg "SDS-Page: Recombinant Human LPAR1/LPA1/EDG-2 Protein [H00001902-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: EDG2 Overexpression Lysate (Adult Normal) [NBP2-05802] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG2.](http://images.novusbio.com/fullsize/EDG2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05802-img0001.jpg "Western Blot: EDG2 Overexpression Lysate (Adult Normal) [NBP2-05802] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG2.")
![Western Blot: EDG2 Overexpression Lysate (Adult Normal) [NBL1-12643] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG2.](http://images.novusbio.com/fullsize/EDG2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12643-img0002.jpg "Western Blot: EDG2 Overexpression Lysate (Adult Normal) [NBL1-12643] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EDG2.")
![Western Blot: LPAR1/LPA1/EDG-2 Antibody (4C3) [NBP3-15186] - Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate. All lanes: LPAR1/LPA1/EDG-2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 42 KDa. Observed band size: 42 KDa.](http://images.novusbio.com/fullsize/LPAR1-LPA1-EDG-2-Antibody-4C3-Western-Blot-NBP3-15186-img0001.jpg "Western Blot: LPAR1/LPA1/EDG-2 Antibody (4C3) [NBP3-15186] - Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate. All lanes: LPAR1/LPA1/EDG-2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 42, 42 KDa. Observed band size: 42 KDa.")
![ELISA: Human LPAR1/LPA1/EDG-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40051] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40051_human-lpar1-lpa1-edg-2-ready-to-use-elisa-kit-color-2110202415362339.png "ELISA: Human LPAR1/LPA1/EDG-2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40051] - Standard Curve Reference")