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![Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NBP1-71867-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hexim 1 Antibody [NBP1-71867] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71868, which recognize other epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71867-img0009.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71867] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71867-img0008.jpg)
![Western Blot: Hexim 1 Antibody [NBP1-71868] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71867, which recognize upstream epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NBP1-71868-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Hexim 1 Antibody [NBP1-71868] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-HEXIM1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71866 and NBP1-71867, which recognize upstream epitopes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Hexim 1 Antibody [NBP1-71868] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71868-img0009.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71868] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71868-img0008.jpg)
![Western Blot: Hexim 1 Antibody [NB100-55391] - Staining of human (A) and mouse (B) heart lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NB100-55391-img0006.jpg "Western Blot: Hexim 1 Antibody [NB100-55391] - Staining of human (A) and mouse (B) heart lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NB100-55391] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55391-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NB100-55391] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-55391-img0007.jpg)
![Western Blot: Hexim 1 Antibody [NBP1-85559] - Analysis in control (vector only transfected HEK293T lysate) and HEXIM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Western-Blot-NBP1-85559-img0008.jpg "Western Blot: Hexim 1 Antibody [NBP1-85559] - Analysis in control (vector only transfected HEK293T lysate) and HEXIM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NBP1-85559] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85559-img0007.jpg)
![Immunohistochemistry-Paraffin: Hexim 1 Antibody [NBP1-85559] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85559-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody (4G4) [H00010614-M01] - Analysis of monoclonal antibody to HEXIM1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-4G4-Immunocytochemistry-Immunofluorescence-H00010614-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody (4G4) [H00010614-M01] - Analysis of monoclonal antibody to HEXIM1 on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: Hexim 1 Antibody (4G4) [H00010614-M01] - Analysis of monoclonal antibody to HEXIM1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-4G4-Immunohistochemistry-Paraffin-H00010614-M01-img0004.jpg)
![Immunohistochemistry: Hexim 1 Antibody [NBP1-71866] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunohistochemistry-NBP1-71866-img0005.jpg "Immunohistochemistry: Hexim 1 Antibody [NBP1-71866] - Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti- HEXIM1 used at a dilution of 1:200 (1ug/ml) ( ) or 1:1,000 (1ug/ml) ( and ). Detection: DAB. Counterstain: hematoxylin (blue).")
![Immunoprecipitation: Hexim 1 Antibody [NBP1-71866] - Whole cell lysate (1 mg for IP, used for IP at 6 mcg/mg lysate. HEXIM1 was also immunoprecipitated by rabbit anti-HEXIM1 antibodies NBP1-71867 and NBP1-71868, which recognize downstream epitopes.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-Immunoprecipitation-NBP1-71866-img0002.jpg)
![Immunohistochemistry-Paraffin: Hexim 1 Antibody (2D12) [H00010614-M02] - Analysis of monoclonal antibody to HEXIM1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-2D12-Immunohistochemistry-Paraffin-H00010614-M02-img0002.jpg "Immunohistochemistry-Paraffin: Hexim 1 Antibody (2D12) [H00010614-M02] - Analysis of monoclonal antibody to HEXIM1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.")
![Sandwich ELISA: Hexim 1 Antibody (2D12) [H00010614-M02] - Detection limit for recombinant GST tagged HEXIM1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-2D12-Sandwich-ELISA-H00010614-M02-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody (1H5) [H00010614-M10] - Analysis of monoclonal antibody to HEXIM1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Hexim-1-Antibody-1H5-Immunocytochemistry-Immunofluorescence-H00010614-M10-img0001.jpg "Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody (1H5) [H00010614-M10] - Analysis of monoclonal antibody to HEXIM1 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: Hexim 1 Antibody (1H5) [H00010614-M10] - Detection limit for recombinant GST tagged HEXIM1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hexim-1-Antibody-1H5-Sandwich-ELISA-H00010614-M10-img0002.jpg)
![Western Blot: HEXIM1 Overexpression Lysate (Adult Normal) [NBL1-11518] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEXIM1.](http://images.novusbio.com/fullsize/HEXIM1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11518-img0002.jpg "Western Blot: HEXIM1 Overexpression Lysate (Adult Normal) [NBL1-11518] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEXIM1.")
![SDS-Page: Recombinant Human Hexim 1 Protein [NBP1-37094] - HEXIM1, 44.8 kDa (396aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/HEXIM1-Protein-SDS-Page-NBP1-37094-img0001.jpg "SDS-Page: Recombinant Human Hexim 1 Protein [NBP1-37094] - HEXIM1, 44.8 kDa (396aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")