Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/FEN-1-Antibody-4E7-Western-Blot-NB100-150-img0006.jpg "Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150] - Analysis of paraformaldehyde-fixed MCF7, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/FEN-1-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NB100-150-img0004.jpg)
![Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody NB100-320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Western-Blot-NB100-320-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody NB100-320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit anti-Fen1 antibody NB100-321, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunoprecipitation-NB100-320-img0007.jpg)
![Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Western-Blot-NB100-321-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: FEN-1 Antibody [NB100-321] - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: FEN-1 Antibody [NB100-321] - FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (NB100-321) used at a dilution of 1:1000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-NB100-321-img0013.jpg)
![Immunoprecipitation: FEN-1 Antibody [NB100-321] - Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody NB100-321 (lot NB100-321-2) used for IP at 6 ug per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-321-1). For blotting immunoprecipitated Fen1, NB100-321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunoprecipitation-NB100-321-img0014.jpg)
![Western Blot: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN-1.](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Western-Blot-NBP2-45640-img0010.jpg "Western Blot: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN-1.")
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0009.jpg)
![Western Blot: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of A549 cell lysate (35ug).](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Western-Blot-NBP2-45640-img0011.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0001.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0002.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0003.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0004.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0005.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0006.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0007.jpg)
![Immunohistochemistry: FEN-1 Antibody (1F3) [NBP2-45640] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/FEN-1-Antibody-1F3-Immunohistochemistry-NBP2-45640-img0008.jpg)
![Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining in human lymph node and kidney tissues using anti-FEN1 antibody. Corresponding FEN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84697-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining in human lymph node and kidney tissues using anti-FEN1 antibody. Corresponding FEN1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: FEN-1 Antibody [NBP1-84697] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/FEN-1-Antibody-Western-Blot-NBP1-84697-img0004.jpg)
![Western Blot: FEN-1 Antibody [NBP1-84697] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/FEN-1-Antibody-Western-Blot-NBP1-84697-img0005.jpg)
![Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84697-img0007.jpg)
![Immunohistochemistry-Paraffin: FEN-1 Antibody [NBP1-84697] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/FEN-1-Antibody-Immunohistochemistry-Paraffin-NBP1-84697-img0008.jpg)
![Western Blot: FEN-1 Antibody (4D9) [NBP3-15190] - Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate. All lanes: FEN-1 antibody at 0.775ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 36 KDa. Observed band size: 43 KDa.](http://images.novusbio.com/fullsize/FEN-1-Antibody-4D9-Western-Blot-NBP3-15190-img0003.jpg "Western Blot: FEN-1 Antibody (4D9) [NBP3-15190] - Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate. All lanes: FEN-1 antibody at 0.775ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43, 36 KDa. Observed band size: 43 KDa.")
![Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4D9) [NBP3-15190] - Immunofluorescence staining of Hela cells with at FEN-1 antibody 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/FEN-1-Antibody-4D9-Immunocytochemistry-Immunofluorescence-NBP3-15190-img0001.jpg)
![Immunohistochemistry: FEN-1 Antibody (4D9) [NBP3-15190] - IHC image of FEN-1 antibody diluted at 1:77.5 and staining in paraffin-embedded human small intestine tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/FEN-1-Antibody-4D9-Immunohistochemistry-NBP3-15190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-274202392427.png "Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-21C-4 (NBP3-20095) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1500 dilution), showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20095, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-21C-4 (NBP3-20095, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924282.png)
![Western Blot: FEN-1 Antibody (SAIC-21C-4) - Azide and BSA Free [NBP3-20095] - Human colon tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-21C-4 (NBP3-20095) at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20095_rabbit-fen-1-mab-saic-21c-4-azide-and-bsa-free-2742023924210.png)
![Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730] - Western blot analysis of extracts of various cell lines, using FEN-1 Rabbit mAb (NBP3-16730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/FEN-1-Antibody-3H9L9-Western-Blot-NBP3-16730-img0002.jpg "Western Blot: FEN-1 Antibody (3H9L9) [NBP3-16730] - Western blot analysis of extracts of various cell lines, using FEN-1 Rabbit mAb (NBP3-16730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: FEN-1 Antibody (3H9L9) [NBP3-16730] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FEN-1 Rabbit mAb (NBP3-16730) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FEN-1-Antibody-3H9L9-Immunohistochemistry-Paraffin-NBP3-16730-img0001.jpg)
![Western Blot: FEN-1 Antibody (S01-3I5) [NBP3-19863] - Western blot detection of FEN-1 in Hela, MCF-7, U251, HL-60 using NBP3-19863 (1:1000 diluted)](http://images.novusbio.com/fullsize/FEN-1-Antibody-S01-3I5-Western-Blot-NBP3-19863-img0001.jpg "Western Blot: FEN-1 Antibody (S01-3I5) [NBP3-19863] - Western blot detection of FEN-1 in Hela, MCF-7, U251, HL-60 using NBP3-19863 (1:1000 diluted)")
![Western Blot: FEN1 Overexpression Lysate (Adult Normal) [NBL1-10676] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FEN1.](http://images.novusbio.com/fullsize/FEN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10676-img0002.jpg "Western Blot: FEN1 Overexpression Lysate (Adult Normal) [NBL1-10676] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FEN1.")
![SDS-Page: FEN1 Protein [NBP1-30306] - FEN-1, 42.5 kDa (380aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE](http://images.novusbio.com/fullsize/FEN1-Protein-SDS-Page-NBP1-30306-img0001.jpg "SDS-Page: FEN1 Protein [NBP1-30306] - FEN-1, 42.5 kDa (380aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE")