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![Western Blot: Cdk9 Antibody (1C4E3) [NBP3-15345] - Western blot analysis of extracts of various cell lines, using Cdk9 Rabbit mAb (NBP3-15345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Western-Blot-NBP3-15345-img0006.jpg "Western Blot: Cdk9 Antibody (1C4E3) [NBP3-15345] - Western blot analysis of extracts of various cell lines, using Cdk9 Rabbit mAb (NBP3-15345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunofluorescence analysis of NIH-3T3 cells using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunocytochemistry-Immunofluorescence-NBP3-15345-img0005.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded mouse brain using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunohistochemistry-Paraffin-NBP3-15345-img0003.jpg)
![Chromatin Immunoprecipitation Sequencing: Cdk9 Antibody (1C4E3) [NBP3-15345] - Chromatin immunoprecipitations were performed with cross-linked chromatin from DLD-1 cells and Cdk9 Rabbit mAb (NBP3-15345). The ChIP sequencing results indicate the enrichment pattern of Cdk9 in selected genomic region and representative gene loci (e.g. RAC1, GAPDH and ACTB), as shown in figure.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Chromatin-Immunoprecipitation-Sequencing-NBP3-15345-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunofluorescence analysis of HeLa cells using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunocytochemistry-Immunofluorescence-NBP3-15345-img0004.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded rat ovary using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunohistochemistry-Paraffin-NBP3-15345-img0001.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunohistochemistry of paraffin-embedded human esophageal using Cdk9 Rabbit mAb (NBP3-15345) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunohistochemistry-Paraffin-NBP3-15345-img0002.jpg)
![Immunoprecipitation: Cdk9 Antibody (1C4E3) [NBP3-15345] - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug Cdk9 antibody (NBP3-15345). Western blot was performed from the immunoprecipitate using Cdk9 antibody (NBP3-15345) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Cdk9-Antibody-1C4E3-Immunoprecipitation-NBP3-15345-img0007.jpg)
![Western Blot: Cdk9 Antibody [NBP2-15848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Western-Blot-NBP2-15848-img0002.jpg "Western Blot: Cdk9 Antibody [NBP2-15848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody [NBP2-15848] - CDK9 antibody [N3C3] detects CDK9 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK9 protein stained by CDK9 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15848-img0003.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody [NBP2-15848] - CDK9 antibody detects CDK9 protein at cytoplasm and nucleus in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. CDK9 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Immunohistochemistry-Paraffin-NBP2-15848-img0005.jpg)
![Western Blot: Cdk9 Antibody [NBP2-15848] - Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Western-Blot-NBP2-15848-img0001.jpg)
![Immunohistochemistry-Paraffin: Cdk9 Antibody [NBP2-15848] - CDK9 antibody detects CDK9 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CDK9 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Immunohistochemistry-Paraffin-NBP2-15848-img0004.jpg)
![Western Blot: Cdk9 Antibody [NB120-10874] - 1) PC3 and 2) DU145 prostate cancer cells (50ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University](http://images.novusbio.com/fullsize/Cdk9-Antibody-Western-Blot-NB120-10874-img0002.jpg "Western Blot: Cdk9 Antibody [NB120-10874] - 1) PC3 and 2) DU145 prostate cancer cells (50ug per lane). Bands at the expected MW of 55 and 42 Kda were detected. Personal communication Flavio Rizzolio, Temple University")
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody [NB120-10874] - Immunocytochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of mcdk9/PITALRE protein in developing mouse tissue. Arrows indicate areas of high expression. Panel A: Peroxidase-DAB immunostaining of mcdk9/PITALRE protein in the developing mouse brain in the differentiated region of the medulla oblongata just below the fourth ventricle. Similar staining is shown in Panel B in the dorsal root ganglia. Panel C: Fluorescein immunofluorescence of mcdk9IPITALRE in skeletal muscle. Similar staining is shown in Panel D in cardiac muscle. Other detection systems should yield similar results.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Immunocytochemistry-Immunofluorescence-NB120-10874-img0001.jpg)
![Western Blot: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of CDK9 expression in transfected 293T cell line by CDK9 monoclonal antibody (M07), clone 2D7. Lane 1: CDK9 transfected lysatE (42.778 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Cdk9-Antibody-2D7-Western-Blot-H00001025-M07-img0006.jpg "Western Blot: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of CDK9 expression in transfected 293T cell line by CDK9 monoclonal antibody (M07), clone 2D7. Lane 1: CDK9 transfected lysatE (42.778 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody (2D7) [H00001025-M07] - Analysis of monoclonal antibody to CDK9 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Cdk9-Antibody-2D7-Immunocytochemistry-Immunofluorescence-H00001025-M07-img0004.jpg)
![ELISA: Cdk9 Antibody (2D7) [H00001025-M07] - Detection limit for recombinant GST tagged CDK9 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cdk9-Antibody-2D7-ELISA-H00001025-M07-img0005.jpg)
![Western Blot: Cdk9 [p Thr29] Antibody [NBP1-77756] - Shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution.](http://images.novusbio.com/fullsize/Cdk9-[p-Thr29]-Antibody-Western-Blot-NBP1-77756-img0002.jpg "Western Blot: Cdk9 [p Thr29] Antibody [NBP1-77756] - Shows detection of phosphorylated CDK9. 100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29. The primary antibody was used at a 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: Cdk9 Antibody [NBP1-86808] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytoplasmic bodies.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86808-img0006.jpg "Immunocytochemistry/Immunofluorescence: Cdk9 Antibody [NBP1-86808] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytoplasmic bodies.")
![Immunohistochemistry-Paraffin: Cdk9 Antibody [NBP1-86808] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Cdk9-Antibody-Immunohistochemistry-Paraffin-NBP1-86808-img0007.jpg)
![Western Blot: Cdk9 [p Thr186] Antibody [NBP3-05455] - Western blot analysis of extracts of NIH/3T3, using Cdk9 antibody (NBP3-05455) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Cdk9-[p-Thr186]-Antibody-Western-Blot-NBP3-05455-img0001.jpg "Western Blot: Cdk9 [p Thr186] Antibody [NBP3-05455] - Western blot analysis of extracts of NIH/3T3, using Cdk9 antibody (NBP3-05455) at 1:1000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Cdk9 Antibody (S08-4F6) [NBP3-19847] - Western blot detection of Cdk9 in Hela, MCF-7, U2OS, U251 using NBP3-19847 (1:1000 diluted)](http://images.novusbio.com/fullsize/Cdk9-Antibody-S08-4F6-Western-Blot-NBP3-19847-img0001.jpg "Western Blot: Cdk9 Antibody (S08-4F6) [NBP3-19847] - Western blot detection of Cdk9 in Hela, MCF-7, U2OS, U251 using NBP3-19847 (1:1000 diluted)")
![SDS-Page: Recombinant Human Cdk9 Protein [H00001025-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001025-P01-1.jpg "SDS-Page: Recombinant Human Cdk9 Protein [H00001025-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![ELISA: Rat Cdk9 ELISA Kit (Colorimetric) [NBP2-75182] - Samples were spiked with high concentrations of Rat Cdk9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75182_rat-cdk9-elisa-kit-colorimetric-132202416232359.jpg "ELISA: Rat Cdk9 ELISA Kit (Colorimetric) [NBP2-75182] - Samples were spiked with high concentrations of Rat Cdk9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Cdk9 ELISA Kit (Colorimetric) [NBP2-75182] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75182_rat-cdk9-elisa-kit-colorimetric-4320249163325.jpg)
![ELISA: Mouse Cdk9 ELISA Kit (Colorimetric) [NBP2-75181] - Samples were spiked with high concentrations of Mouse Cdk9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75181_mouse-cdk9-elisa-kit-colorimetric-13220241692243.jpg "ELISA: Mouse Cdk9 ELISA Kit (Colorimetric) [NBP2-75181] - Samples were spiked with high concentrations of Mouse Cdk9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Cdk9 ELISA Kit (Colorimetric) [NBP2-75181] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75181_mouse-cdk9-elisa-kit-colorimetric-432024973648.jpg)