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![Immunocytochemistry/Immunofluorescence: CCDC8 Antibody [NBP1-85085] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85085-img0006.jpg "Immunocytochemistry/Immunofluorescence: CCDC8 Antibody [NBP1-85085] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.")
![Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP1-85085] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Immunohistochemistry-Paraffin-NBP1-85085-img0002.jpg)
![Western Blot: CCDC8 Antibody [H00083987-B01P] - Analysis of CCDC8 expression in rat brain.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Western-Blot-H00083987-B01P-img0002.jpg "Western Blot: CCDC8 Antibody [H00083987-B01P] - Analysis of CCDC8 expression in rat brain.")
![Western Blot: CCDC8 Antibody [H00083987-B01P] - Analysis of CCDC8 expression in human spleen.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Western-Blot-H00083987-B01P-img0001.jpg)
![Western Blot: CCDC8 Antibody [H00083987-B01P] - Analysis of CCDC8 expression in transfected 293T cell line by CCDC8 polyclonal antibody. Lane 1: CCDC8 transfected lysate(59.40 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Western-Blot-H00083987-B01P-img0003.jpg)
![Western Blot: CCDC8 Antibody [NBP1-50021] - Detection of Human CCDC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated CCDC8, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).](http://images.novusbio.com/fullsize/CCDC8-Antibody-Western-Blot-NBP1-50021-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CCDC8 Antibody [NBP1-50021] - Detection of Human CCDC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated CCDC8, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).")
![Immunoprecipitation: CCDC8 Antibody [NBP1-50022] - Detection of Human CCDC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes an upstream epitope. For blotting immunoprecipitated CCDC8 was used at 0.4 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 10 seconds.Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Immunoprecipitation-NBP1-50022-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: CCDC8 Antibody [NBP1-50022] - Detection of Human CCDC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes an upstream epitope. For blotting immunoprecipitated CCDC8 was used at 0.4 mcg/ml, and the ReliaBLOT Reagents and Procedures were incorporated. Detection: Chemiluminescence with an exposure time of 10 seconds.Chemiluminescence with an exposure time of 10 seconds.")
![Western Blot: CCDC8 Overexpression Lysate (Adult Normal) [NBL1-08824] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCDC8.](http://images.novusbio.com/fullsize/CCDC8-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08824-img0002.jpg "Western Blot: CCDC8 Overexpression Lysate (Adult Normal) [NBL1-08824] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCDC8.")
![Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP3-06010] - Immunochemical staining of human CCDC8 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/CCDC8-Antibody-Immunohistochemistry-Paraffin-NBP3-06010-img0001.jpg "Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP3-06010] - Immunochemical staining of human CCDC8 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")