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![Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Western-Blot-NBP1-22439-img0015.jpg "Western Blot: Cav1.2 Antibody (S57) [NBP1-22439] - Western Blot analysis of Hamster T-CHO cell lysate showing detection of CaV1.2 Calcium Channel protein using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0018.jpg)
![Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Brain Tissue. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium channel Monoclonal Antibody (NBP1-22439) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunohistochemistry-NBP1-22439-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody (S57) [NBP1-22439] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.2 Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav1.2 Monoclonal Antibody (NBP1-22439) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-Cav1.2 Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav1.2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunocytochemistry-Immunofluorescence-NBP1-22439-img0019.jpg)
![Immunohistochemistry: Cav1.2 Antibody (S57) [NBP1-22439] - Immunohistochemistry analysis using Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody, Clone S57 (NBP1-22439). Tissue: hippocampus. Species: Human. Fixation: 10% formalin. Primary Antibody: Mouse Anti-CaV1.2 Calcium Channel Monoclonal Antibody (NBP1-22439) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-S57-Immunohistochemistry-NBP1-22439-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - Staining of CACNA1C in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76914-img0008.jpg "Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - Staining of CACNA1C in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0006.jpg)
![Flow Cytometry: Cav1.2 Antibody [NBP2-76914] - Flow cytometric analysis of SKOV-3 cells with CACNA1C antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Flow-Cytometry-NBP2-76914-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - ICC staining CACNA1C in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76914-img0002.jpg)
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0003.jpg)
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0004.jpg)
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Immunohistochemistry-Paraffin-NBP2-76914-img0005.jpg)
![Dot Blot: Cav1.2 Antibody [NBP2-76914] - Dot blot analysis of anti-CACNA1C on PVDF. 1ug, 2ug and 4ug of immunization peptides were given in this test. Anti-CACNA1C antibody was diluted with 1/500.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Dot-Blot-NBP2-76914-img0001.jpg)
![Western Blot: Cav1.2 Antibody [NBP1-20896] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cav1.2-Antibody-Western-Blot-NBP1-20896-img0004.jpg "Western Blot: Cav1.2 Antibody [NBP1-20896] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP3-14555] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, cortex tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Cav1.2 Antibody-Immunohistochemistry-Paraffin-NBP3-14555-img0001.jpg "Immunohistochemistry-Paraffin: Cav1.2 Antibody [NBP3-14555] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, cortex tissue after heat-induced antigen retrieval.")
![ELISA: Cav1.2 Antibody (4D10) [H00000775-M20] - Detection limit for recombinant GST tagged CACNA1C is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cav1-2-Antibody-4D10-ELISA-H00000775-M20-img0001.jpg "ELISA: Cav1.2 Antibody (4D10) [H00000775-M20] - Detection limit for recombinant GST tagged CACNA1C is 0.03 ng/ml as a capture antibody.")