| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB200-607-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2184-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2184-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-77742-0.1mg | |||
| HRP | NB600-619-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03387-20ul | |||
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0008.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Differential expression of CAIX and CAXII in patient-derived tumor grafts. Frozen tissue samples from PDX tumors were homogenized in RIPA buffer containing protease inhibitor. Western blots were probed with antibodies for CAIX, CAXII, CAII, ER, E-cadherin, actin, and GAPDH. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0199476) licensed under a CC-BY license.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Staining in nucleus and cytoplasm of proximal and distal tubules, bowman's capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 ug/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3'3-diamino-benzidine substrate (DAB) or WARP RED.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-NB600-919-img0005.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Detection of Carbonic Anhydrase II in mouse embryonic whole cell lysate. Image provided via product review by verified customer.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0001.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - analysis of Carbonic Anhydrase II protein (loading amount: 50 ng) using rabbit anti-Carbonic Anhydrase II antibody (NB600-919) at a dilution of 1:1000, incubation - overnight at 4C.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0002.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Analysis using the Biotin conjugate of NB600-919. Detection of Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptav](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0004.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic Anhydrase II primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II. Other band(s): None.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-919-img0007.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NB600-919] - Localization of CAII in mouse peritoneum. Image from verified customer review.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-Paraffin-NB600-919-img0003.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic AnhydraseII primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Alkaline Phosphatase. Other band(s): None.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NB600-943-img0002.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Lane 1: Carbonic Anhydrase II. Lane 2: None. Load: 50 ng per lane. Primary antibody: Carbonic AnhydraseII primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for Alkaline Phosphatase. Other band(s): None.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody [NB600-943] - Tissue: intestine Staining: diffuse positivity of enzyma Carbonic Anidrase II in brush border of villus.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-NB600-943-img0001.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Analysis in human cell lines HEK293 and PC-3 using Anti-CA2 antibody. Corresponding CA2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NBP2-52736-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Analysis in human cell lines HEK293 and PC-3 using Anti-CA2 antibody. Corresponding CA2 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Staining of human duodenum shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Immunohistochemistry-Paraffin-NBP2-52736-img0003.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody [NBP2-52736] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Western-Blot-NBP2-52736-img0001.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-278202415242692.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Western blot analysis of Carbonic Anhydrase II/CA2 on different lysates with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32105) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32105) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29820241420924.jpg)
![Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase II/CA2 Antibody (11A2-R) [NBP3-32105] - Immunocytochemistry analysis of THP-1 cells labeling Carbonic Anhydrase II/CA2 with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Carbonic Anhydrase II/CA2 antibody (NBP3-32105) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32105_mouse-carbonic-anhydrase-ii-ca2-mab-11a2-r-29202410334053.jpg)
![Western Blot: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDa Observed band size:30 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-001-Western-Blot-NBP2-89522-img0001.jpg "Western Blot: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522] - Lane A: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:29 kDa Observed band size:30 kDa")
![Immunoprecipitation: Carbonic Anhydrase II/CA2 Antibody (001) [NBP2-89522] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-CA2 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CA2 rabbit monoclonal antibody,at 1:2000 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa Observed band size: 29 kDa](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-001-Immunoprecipitation-NBP2-89522-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0004.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse stomach with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0001.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase II/CA2 Antibody (003) [NBP2-90677] - Immunochemical staining of mouse CA2 in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-003-Immunohistochemistry-Paraffin-NBP2-90677-img0003.jpg)
![ELISA: Mouse Carbonic Anhydrase II/CA2 ELISA Kit (Colorimetric) [NBP2-70055] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase II/CA2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70055_mouse-carbonic-anhydrase-ii-ca2-elisa-kit-colorimetric-1322024168532.jpg "ELISA: Mouse Carbonic Anhydrase II/CA2 ELISA Kit (Colorimetric) [NBP2-70055] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase II/CA2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Carbonic Anhydrase II/CA2 ELISA Kit (Colorimetric) [NBP2-70055] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70055_mouse-carbonic-anhydrase-ii-ca2-elisa-kit-colorimetric-432024911567.jpg)
![Knockout Validated: Carbonic Anhydrase II/CA2 Antibody [NBP3-03387] - Analysis of extracts from normal (control) and CA2 knockout (KO) 293T cells, using Carbonic Anhydrase II/CA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Antibody-Knockout-Validated-NBP3-03387-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Carbonic Anhydrase II/CA2 Antibody [NBP3-03387] - Analysis of extracts from normal (control) and CA2 knockout (KO) 293T cells, using Carbonic Anhydrase II/CA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer 3% nonfat dry milk in TBST.")
![SDS-Page: Recombinant E. coli Carbonic Anhydrase II/CA2 Protein [NBP2-52240] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-II-CA2-Recombinant-Protein-SDS-Page-NBP2-52240-img0001.jpg "SDS-Page: Recombinant E. coli Carbonic Anhydrase II/CA2 Protein [NBP2-52240] - 15% SDS Page (3 ug)")