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![Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30475-img0024.jpg "Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30475-img0021.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-30475] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (NBP1-30475). Detection: DAB](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-30475-img0020.jpg)
![Western Blot: BRD2 Antibody [NBP1-30475] - Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibodyused for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30475-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30475] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30474 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP2-76410 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30475-img0022.jpg)
![Western Blot: BRD2 Antibody [NBP1-84310] - Analysis in human cell lines HEK293 and MCF-7 using Anti-BRD2 antibody. Corresponding BRD2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-84310-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: BRD2 Antibody [NBP1-84310] - Analysis in human cell lines HEK293 and MCF-7 using Anti-BRD2 antibody. Corresponding BRD2 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-84310] - Staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0014.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-84310] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0012.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-84310] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0009.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-84310] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0010.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-84310] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-84310-img0011.jpg)
![Western Blot: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30474-img0005.jpg "Western Blot: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30474] - Formaldehyde-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:100 (2ug/ml) .](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30474-img0002.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: BRD2 Antibody [NBP1-30474] - Whole cell lysate from HeLa and 293T cells. NBP1-30474 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. BRD2 was also IPed by rabbit anti-BRD2 antibody NBP1-30475.](http://images.novusbio.com/fullsize/BRD2-Antibody-Western-Blot-NBP1-30474-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30475 and rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (NBP2-76410). For blotting immunoprecipitated BRD2, NBP1-30474 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30474-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody [NBP1-30474] - Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody NBP1-30474 (lot NBP1-30474-2) used for IP at 6 ug per reaction. BRD2 was also immunoprecipitated by rabbit anti-BRD2 antibody NBP1-30475. For blotting immunoprecipitated BRD2, NBP1-30474 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-Immunoprecipitation-NBP1-30474-img0006.jpg)
![Western Blot: BRD2 Antibody (7C1B10) [NBP2-61716] - Analysis using BRD2 mAb against HEK293 (1) and BRD2 (AA: 227-364)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BRD2-Antibody-7C1B10-Western-Blot-NBP2-61716-img0004.jpg "Western Blot: BRD2 Antibody (7C1B10) [NBP2-61716] - Analysis using BRD2 mAb against HEK293 (1) and BRD2 (AA: 227-364)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: BRD2 Antibody (7C1B10) [NBP2-61716] - Analysis of Hela cells using BRD2 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BRD2-Antibody-7C1B10-Flow-Cytometry-NBP2-61716-img0002.jpg)
![Western Blot: BRD2 Antibody (7C1B10) [NBP2-61716] - Analysis using BRD2 mAb against human BRD2 (AA: 227-364) recombinant protein. (Expected MW is 40.6 kDa)](http://images.novusbio.com/fullsize/BRD2-Antibody-7C1B10-Western-Blot-NBP2-61716-img0003.jpg)
![Western Blot: BRD2 Antibody (7C1B10) [NBP2-61716] - Analysis using BRD2 mouse mAb against C6 (1) and Hela (2) cell lysate.](http://images.novusbio.com/fullsize/BRD2-Antibody-7C1B10-Western-Blot-NBP2-61716-img0005.jpg)
![ELISA: BRD2 Antibody (7C1B10) [NBP2-61716] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/BRD2-Antibody-7C1B10-ELISA-NBP2-61716-img0001.jpg)
![Western Blot: BRD2 Antibody (BL-167-2A2) [NBP2-76410] - Whole cell lysate (50 ug) from RenCa, NIH 3T3, and C2C12cells prepared using NETN lysis buffer. Antibody: Rabbitanti-BRD2 recombinant monoclonal antibody [BL-167-2A2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BRD2-Antibody-BL-167-2A2-Western-Blot-NBP2-76410-img0004.jpg "Western Blot: BRD2 Antibody (BL-167-2A2) [NBP2-76410] - Whole cell lysate (50 ug) from RenCa, NIH 3T3, and C2C12cells prepared using NETN lysis buffer. Antibody: Rabbitanti-BRD2 recombinant monoclonal antibody [BL-167-2A2] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: BRD2 Antibody (BL-167-2A2) [NBP2-76410] - Whole cell lysate (50 ug) from HEK293T, MCF-7, Hep-G2,A-549, SW620, SK-MEL-28, and Jurkat cells preparedusing NETN lysis buffer. Antibody: Rabbit anti-BRD2recombinant monoclonal antibody [BL-167-2A2] used at 1:1000. Secondary: HRPconjugatedgoat anti-rabbit IgG Chemiluminescence with an exposure time of 30 seconds.Lower Panel: Rabbit anti-Actin.](http://images.novusbio.com/fullsize/BRD2-Antibody-BL-167-2A2-Western-Blot-NBP2-76410-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody (BL-167-2A2) [NBP2-76410] - Detection of human BRD2 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-BRD2 recombinant monoclonal [BL-167-2A2] (NBP2-76410) and another rabbit anti-BRD2 antibody.](http://images.novusbio.com/fullsize/BRD2-Antibody-BL-167-2A2-Immunoprecipitation-NBP2-76410-img0002.jpg)
![Western Blot: BRD2 Antibody (3D10) [H00006046-M01] - BRD2 monoclonal antibody (M01), clone 3D10. Analysis of BRD2 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/BRD2-Antibody-3D10-Western-Blot-H00006046-M01-img0004.jpg "Western Blot: BRD2 Antibody (3D10) [H00006046-M01] - BRD2 monoclonal antibody (M01), clone 3D10. Analysis of BRD2 expression in Hela S3 NE.")
![ELISA: BRD2 Antibody (3D10) [H00006046-M01] - Detection limit for recombinant GST tagged BRD2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BRD2-Antibody-3D10-ELISA-H00006046-M01-img0003.jpg)
![Immunoprecipitation: BRD2 Antibody (12C2E4) [NBP2-14861] - Detection of human BRD2 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Mouse monoclonal anti-BRD2 [12C2E4] (NBP2-14861) and rabbit anti-BRD2 antibody (NBP1-30474). Secondary: HRP-conjugated goat anti-mouse IgG.](http://images.novusbio.com/fullsize/BRD2-Antibody-12C2E4-Immunoprecipitation-NBP2-14861-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: BRD2 Antibody (12C2E4) [NBP2-14861] - Detection of human BRD2 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Mouse monoclonal anti-BRD2 [12C2E4] (NBP2-14861) and rabbit anti-BRD2 antibody (NBP1-30474). Secondary: HRP-conjugated goat anti-mouse IgG.")
![<b>Independent Antibodies Validation. </b>Western Blot: BRD2 Antibody (12C2E4) [NBP2-14861] - Whole cell lysate (50 µg) from HEK293T, HeLa, MCF-7,Hep-G2, and Jurkat cells prepared using NETN lysis buffer.Antibody: Mouse anti-BRD2 monoclonal antibody [12C2E4] used at 1:1000. Secondary: HRP conjugated goat anti-mouse IgG. Chemiluminescence with an exposure time of 30 seconds.Lower Panel: Rabbit anti-COPB2 antibody .](http://images.novusbio.com/fullsize/antibody/nbp2-14861_mouse-monoclonal-brd2-antibody-12c2e4-western-blot-2211202491436..png)
![ELISA: BRD2 Antibody [NBP3-18662] - Figure 2. Western blot analysis using the antibody directed against Brd2 To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Brd2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.](http://images.novusbio.com/fullsize/BRD2 Antibody-ELISA-NBP3-18662-img0002.jpg "ELISA: BRD2 Antibody [NBP3-18662] - Figure 2. Western blot analysis using the antibody directed against Brd2 To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Brd2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.")
![ELISA: BRD2 Antibody [NBP3-18662] - Figure 1. Determination of the antibody titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse Brd2 . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,000.](http://images.novusbio.com/fullsize/BRD2-Antibody-ELISA-NBP3-18662-img0001.jpg)
![Western Blot: BRD2 Antibody (5K5Y6) [NBP3-33404] - Western blot analysis of various lysates, using BRD2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33404_rabbit-brd2-mab-5k5y6-912202415171139.jpg "Western Blot: BRD2 Antibody (5K5Y6) [NBP3-33404] - Western blot analysis of various lysates, using BRD2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.")
![SDS-Page: Recombinant Human BRD2 Protein [NBP2-51885] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/BRD2-Recombinant-Protein-SDS-Page-NBP2-51885-img0001.jpg "SDS-Page: Recombinant Human BRD2 Protein [NBP2-51885] - 15% SDS Page (3 ug)")