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![Knockout Validated: ARID2 Antibody (7311) [NBP2-43567] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody. (WT: wild type; KO: knockout)](http://images.novusbio.com/fullsize/ARID2-Antibody-7311-Western-Blot-NBP2-43567-img0002.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: ARID2 Antibody (7311) [NBP2-43567] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody. (WT: wild type; KO: knockout)")
![<b>Genetic Strategies Validation. </b>Western Blot: ARID2 Antibody (7311) [NBP2-43567] - Non-transfected (-) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (7311) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ARID2-Antibody-7311-Western-Blot-NBP2-43567-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ARID2 Antibody (7311) [NBP2-43567] - detects ARID2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ARID2 stained by diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500. Scale bar= 10um.](http://images.novusbio.com/fullsize/ARID2-Antibody-7311-Immunocytochemistry-Immunofluorescence-NBP2-43567-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ARID2 Antibody (7311) [NBP2-43567] - ARID2 antibody validation by shRNA knockdown. Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody.](http://images.novusbio.com/fullsize/ARID2-Antibody-7311-Western-Blot-NBP2-43567-img0003.jpg)
![Western Blot: ARID2 Antibody (7311) [NBP2-43567] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody [GT7311] (NBP2-43567) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43567_mouse-monoclonal-arid2-antibody-7311-91020242075949.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ARID2 Antibody (7311) [NBP2-43567] - Non-transfected (–) and transfected (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody [GT7311] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43567_mouse-monoclonal-arid2-antibody-7311-1610202419414563.jpg)
![Western Blot: ARID2 Antibody [NBP3-12715] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:197 kDaObserved band size:250 kDa](http://images.novusbio.com/fullsize/ARID2-Antibody-Western-Blot-NBP3-12715-img0002.jpg "Western Blot: ARID2 Antibody [NBP3-12715] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:197 kDaObserved band size:250 kDa")
![Immunocytochemistry/Immunofluorescence: ARID2 Antibody [NBP3-12715] - Staining of ARID2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ARID2 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12715-img0003.jpg)
![Immunoprecipitation: ARID2 Antibody [NBP3-12715] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-ARID2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ARID2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 197 kDaObserved band size :250 kDa](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunoprecipitation-NBP3-12715-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ARID2 Antibody [NBP2-57220] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57220-img0001.jpg "Immunocytochemistry/Immunofluorescence: ARID2 Antibody [NBP2-57220] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Western Blot: ARID2 Antibody [NBP2-92943] - Analysis of extracts of LO2 cells, using ARID2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s](http://images.novusbio.com/fullsize/ARID2-Antibody-Western-Blot-NBP2-92943-img0002.jpg "Western Blot: ARID2 Antibody [NBP2-92943] - Analysis of extracts of LO2 cells, using ARID2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s")
![Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/ARID2-Antibody-Western-Blot-NBP1-26615-img0006.jpg "Western Blot: ARID2 Antibody [NBP1-26615] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunohistochemistry-Paraffin-NBP1-26615-img0008.jpg)
![Immunohistochemistry-Paraffin: ARID2 Antibody [NBP1-26615] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ARID2 used at a dilution of 1:5,000 ( 0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunohistochemistry-Paraffin-NBP1-26615-img0007.jpg)
![Immunoprecipitation: ARID2 Antibody [NBP1-26615] - Mouse embryonic fibroblasts. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunoprecipitation-NBP1-26615-img0003.jpg)
![Immunoprecipitation: ARID2 Antibody [NBP1-26615] - Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID2 antibody NBP1-26615 (lot NBP1-26615-2) used for IP at 6 ug per reaction. ARID2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26615-1). For blotting immunoprecipitated ARID2, NBP1-26615 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunoprecipitation-NBP1-26615-img0009.jpg)
![Western Blot: ARID2 Antibody [NBP1-26614] - Detection of human ARID2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody NBP1-26614 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ARID2-Antibody-Western-Blot-NBP1-26614-img0003.jpg "Western Blot: ARID2 Antibody [NBP1-26614] - Detection of human ARID2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID2 antibody NBP1-26614 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: ARID2 Antibody [NBP1-26614] - Detection of human ARID2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID2 antibody NBP1-26614 (lot NBP1-26614-2) used for IP at 6 ug per reaction. ARID2 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-26614-1) and rabbit anti-ARID2 antibody NBP1-26615. For blotting immunoprecipitated ARID2, NBP1-26614 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ARID2-Antibody-Immunoprecipitation-NBP1-26614-img0004.jpg)