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![Western Blot: Aly Antibody (JE36-45) [NBP3-32033] - Western blot analysis of Aly on different lysates with Rabbit anti-Aly antibody (NBP3-32033) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: SK-OV-3 cell lysate (20 ug/Lane) Lane 6: HL-60 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Neuro-2a cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: Mouse testis tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-278202416285215.jpg "Western Blot: Aly Antibody (JE36-45) [NBP3-32033] - Western blot analysis of Aly on different lysates with Rabbit anti-Aly antibody (NBP3-32033) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: A549 cell lysate (20 ug/Lane) Lane 5: SK-OV-3 cell lysate (20 ug/Lane) Lane 6: HL-60 cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Neuro-2a cell lysate (20 ug/Lane) Lane 9: C6 cell lysate (20 ug/Lane) Lane 10: Mouse testis tissue lysate (40 ug/Lane) Lane 11: Mouse spleen tissue lysate (40 ug/Lane) Lane 12: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32033) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Aly Antibody (JE36-45) [NBP3-32033] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Aly antibody (NBP3-32033) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32033) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-29820241358555.jpg)
![Immunocytochemistry/ Immunofluorescence: Aly Antibody (JE36-45) [NBP3-32033] - Immunocytochemistry analysis of HepG2 cells labeling Aly with Rabbit anti-Aly antibody (NBP3-32033) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aly antibody (NBP3-32033) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-29202410334059.jpg)
![Flow Cytometry: Aly Antibody (JE36-45) [NBP3-32033] - Flow cytometric analysis of HepG2 cells labeling Aly. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32033, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32033_rabbit-aly-mab-je36-45-29202416372142.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody [NBP1-33598] - Human esophageal cancer ALY antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Aly-Antibody-Immunohistochemistry-Paraffin-NBP1-33598-img0009.jpg "Immunohistochemistry-Paraffin: Aly Antibody [NBP1-33598] - Human esophageal cancer ALY antibody diluted at 1:500.")
![Western Blot: Aly Antibody [NBP1-33598] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Aly-Antibody-Western-Blot-NBP1-33598-img0006.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody [NBP1-33598] - Human cervical carcinoma: Paraffin-embedded human cervical carcinoma diluted at 1:500.](http://images.novusbio.com/fullsize/Aly-Antibody-Immunohistochemistry-Paraffin-NBP1-33598-img0008.jpg)
![Western Blot: Aly Antibody [NBP1-33598] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Aly antibody (NBP1-33598) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp1-33598_rabbit-polyclonal-aly-antibody-91020242075973.jpg)
![Western Blot: Aly Antibody (11G5) [NB100-670] - La cell extract was separated by SDS-PAGE and probed with Monoclonal Anti-ALY (0.4 ug/mL, lane 1) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, lane 1,2) and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/Aly-Antibody-11G5-Western-Blot-NB100-670-img0002.jpg "Western Blot: Aly Antibody (11G5) [NB100-670] - La cell extract was separated by SDS-PAGE and probed with Monoclonal Anti-ALY (0.4 ug/mL, lane 1) followed by Goat Anti-Mouse, Peroxidase conjugate (Cat. No. A2304, lane 1,2) and a chemiluminescent substrate.")
![Western Blot: Aly Antibody (6I4T10) [NBP3-16372] - Western blot anTHOC4/AlyREFsis of extracts of various cell lines, using THOC4/AlyREF Rabbit mAb (NBP3-16372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Western-Blot-NBP3-16372-img0004.jpg "Western Blot: Aly Antibody (6I4T10) [NBP3-16372] - Western blot anTHOC4/AlyREFsis of extracts of various cell lines, using THOC4/AlyREF Rabbit mAb (NBP3-16372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Aly Antibody (6I4T10) [NBP3-16372] - Immunohistochemistry of paraffin-embedded mouse brain using THOC4/AlyREF Rabbit mAb (NBP3-16372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Immunohistochemistry-Paraffin-NBP3-16372-img0003.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody (6I4T10) [NBP3-16372] - Immunohistochemistry of paraffin-embedded rat brain using THOC4/AlyREF Rabbit mAb (NBP3-16372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Immunohistochemistry-Paraffin-NBP3-16372-img0001.jpg)
![Immunohistochemistry-Paraffin: Aly Antibody (6I4T10) [NBP3-16372] - Immunohistochemistry of paraffin-embedded human esophageal using THOC4/AlyREF Rabbit mAb (NBP3-16372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aly-Antibody-6I4T10-Immunohistochemistry-Paraffin-NBP3-16372-img0002.jpg)
![Western Blot: Aly Antibody (SR1439) [NBP3-22102] - Western blot analysis using NBP3-22102 in HEK293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22102_rabbit-aly-mab-sr1439-57202316494211.jpg "Western Blot: Aly Antibody (SR1439) [NBP3-22102] - Western blot analysis using NBP3-22102 in HEK293 cell lysate.")
![Western Blot: Aly Antibody (2G8) [H00010189-M03] - Analysis of ALYREF expression in Jurkat.](http://images.novusbio.com/fullsize/Aly-Antibody-2G8-Western-Blot-H00010189-M03-img0003.jpg "Western Blot: Aly Antibody (2G8) [H00010189-M03] - Analysis of ALYREF expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: Aly Antibody (2G8) [H00010189-M03] - Analysis of monoclonal antibody to ALYREF on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Aly-Antibody-2G8-Immunocytochemistry-Immunofluorescence-H00010189-M03-img0001.jpg)
![Sandwich ELISA: Aly Antibody (2G8) [H00010189-M03] - Detection limit for recombinant GST tagged ALYREF is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aly-Antibody-2G8-Sandwich-ELISA-H00010189-M03-img0002.jpg)
![Western Blot: Aly Antibody [NBP2-56154] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Aly-Antibody-Western-Blot-NBP2-56154-img0002.jpg "Western Blot: Aly Antibody [NBP2-56154] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: Aly Antibody [NBP2-56154] - Staining of human cell line U-2 OS shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/Aly-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56154-img0001.jpg)
![Western Blot: Aly Antibody [NBP3-10855] - Western blot analysis of Aly in Mouse Pancreas lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/Aly-Antibody-Western-Blot-NBP3-10855-img0001.jpg "Western Blot: Aly Antibody [NBP3-10855] - Western blot analysis of Aly in Mouse Pancreas lysates. Antibody dilution at 1ug/ml")
![ELISA: Human Aly ELISA Kit (Colorimetric) [NBP3-38951] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38951_human-aly-elisa-kit-colorimetric-2110202415351249.png "ELISA: Human Aly ELISA Kit (Colorimetric) [NBP3-38951] - Standard Curve Reference")
![ELISA: Human Aly - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38950] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-38950_human-aly-ready-to-use-elisa-kit-colorimetric-2110202415292795.png "ELISA: Human Aly - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38950] - Standard Curve Reference")