| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41308-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92681-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03374-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41308 | |||
| BSA Free | NBP2-41308-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nbp1-36939_goat-polyclonal-atg4d-antibody-immunocytochemistry-immunofluorescence-283202416418..jpg "Immunocytochemistry/Immunofluorescence: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-36939] - Staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-36939-img0001.jpg)
![Flow Cytometry: Goat Polyclonal ATG4D Antibody [NBP1-36939] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-36939_goat-polyclonal-atg4d-antibody-flow-cytometry-283202416538..jpg)
![Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Western-Blot-NBP2-41308-img0001.jpg "Western Blot: ATG4D Antibody [NBP2-41308] - Analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ATG4D Antibody - BSA Free [NBP2-41308] - Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202420115547.jpg)
![Immunohistochemistry: ATG4D Antibody - BSA Free [NBP2-41308] - Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41308_rabbit-polyclonal-atg4d-antibody-2711202419592031.jpg)
![Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33488-img0006.jpg "Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP1-33488] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-33488-img0005.jpg)
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded SAS xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-33488-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG4D Antibody [NBP1-33488] - Paraffin-embedded mouse ovary. ATG4D antibody [N3C3] diluted at 1:500.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-Paraffin-NBP1-33488-img0004.jpg)
![Western Blot: ATG4D Antibody [NBP3-37970] - Western blot analysis of various lysates using ATG4D Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-37970_rabbit-atg4d-pab-91220241519581.jpg "Western Blot: ATG4D Antibody [NBP3-37970] - Western blot analysis of various lysates using ATG4D Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: ATG4D Antibody [NBP3-37970] - Immunofluorescence analysis of U2OS cells using ATG4D Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37970_rabbit-atg4d-pab-912202414201379.jpg)
![Western Blot: ATG4D Antibody [NBP2-92681] - Analysis of extracts of various cell lines, using ATG4D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Western-Blot-NBP2-92681-img0002.jpg "Western Blot: ATG4D Antibody [NBP2-92681] - Analysis of extracts of various cell lines, using ATG4D at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: ATG4D Antibody [NBP2-92681] - Analysis of U2OS cells using ATG4D at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92681-img0001.jpg)
![Western Blot: ATG4D Antibody [NBP3-03374] - Analysis of extracts from normal (control) and ATG4D knockout (KO) HeLa cells, using ATG4D antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Western-Blot-NBP3-03374-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG4D Antibody [NBP3-03374] - Analysis of extracts from normal (control) and ATG4D knockout (KO) HeLa cells, using ATG4D antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![SDS-Page: Recombinant Human ATG4D Protein [H00084971-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00084971-P01-1.jpg "SDS-Page: Recombinant Human ATG4D Protein [H00084971-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry: ATG4D Antibody [NBP2-49525] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG4D-Antibody-Immunohistochemistry-NBP2-49525-img0001.jpg "Immunohistochemistry: ATG4D Antibody [NBP2-49525] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.")