Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ce,
Dr,
Gp,
Pl,
Pm,
Rb,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ce, Dr, Gp, Pl, Pm, Rb, Ye
WB, Flow, ICC/IF, IHC, IP
Applications: WB, Flow, ICC/IF, IHC, IP
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - TCP1 monoclonal antibody (M01), clone 2B2-D6 Analysis of TCP1 expression in Hela.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-2B2-D6-Western-Blot-H00006950-M01-img0010.jpg "Western Blot: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - TCP1 monoclonal antibody (M01), clone 2B2-D6 Analysis of TCP1 expression in Hela.")
![Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - Analysis of monoclonal antibody to TCP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-2B2-D6-Immunocytochemistry-Immunofluorescence-H00006950-M01-img0006.jpg)
![Immunohistochemistry-Paraffin: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - Analysis of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-2B2-D6-Immunohistochemistry-Paraffin-H00006950-M01-img0008.jpg)
![ELISA: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - Detection limit for recombinant GST tagged TCP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-2B2-D6-Sandwich-ELISA-H00006950-M01-img0009.jpg)
![Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NB100-1400-img0004.jpg "Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1400-img0005.jpg)
![Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NB100-1400] - Staining of paraffin embedded Human Testis. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunohistochemistry-Paraffin-NB100-1400-img0002.jpg)
![Flow Cytometry: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Flow-Cytometry-NB100-1400-img0006.jpg)
![Western Blot: TCP1 alpha Antibody (91a) [NBP1-97505] - Analysis of CTA-191: Lane 1: MW marker, Lane 2: HeLa Cell Lysate, Heat shocked, Lane 3: PC-12 Cell Lysate Heat Shocked, Lane 4: 3T3 Cell Lysate, Heat Shocked, Lane 5, RK-13 Cell Lysate, Heat Shocked.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-91a-Western-Blot-NBP1-97505-img0001.jpg "Western Blot: TCP1 alpha Antibody (91a) [NBP1-97505] - Analysis of CTA-191: Lane 1: MW marker, Lane 2: HeLa Cell Lysate, Heat shocked, Lane 3: PC-12 Cell Lysate Heat Shocked, Lane 4: 3T3 Cell Lysate, Heat Shocked, Lane 5, RK-13 Cell Lysate, Heat Shocked.")
![Flow Cytometry: TCP1 alpha Antibody (91a) [NBP1-97505] - Analysis human colon cancer Coca-2 cells were analyzed by FLOW cytometry using isotype control antibody (left) or TCP-1alpha antibody, clone #91a (right).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-91a-Flow-Cytometry-NBP1-97505-img0002.jpg)
![Western Blot: TCP1 alpha Antibody (23c) [NB120-13501] Lane 1: MW marker, Lane 2: Hsp60 Recombinant Human Protein, Lane 3: 3T3 Heat Shocked, Lane 4: PC-12 Heat Shocked.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-23c-Western-Blot-NB120-13501-img0001.jpg "Western Blot: TCP1 alpha Antibody (23c) [NB120-13501] Lane 1: MW marker, Lane 2: Hsp60 Recombinant Human Protein, Lane 3: 3T3 Heat Shocked, Lane 4: PC-12 Heat Shocked.")
![Western Blot: TCP1 alpha Antibody [NBP1-88148] - Analysis using Anti-TCP1 antibody NBP1-88148 (A) shows similar pattern to independent antibody NBP1-88149 (B).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NBP1-88148-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TCP1 alpha Antibody [NBP1-88148] - Analysis using Anti-TCP1 antibody NBP1-88148 (A) shows similar pattern to independent antibody NBP1-88149 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NBP1-88148] - Staining of human testis shows distinct cytoplasmic positivity in seminiferus duct cells.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-88148-img0004.jpg)
![Western Blot: TCP1 alpha Antibody [NBP1-88148] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NBP1-88148-img0008.jpg)
![Immunohistochemistry: TCP1 alpha Antibody [NBP3-35337] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TCP1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35337_rabbit-tcp1-alpha-pab-912202414201347.jpg "Immunohistochemistry: TCP1 alpha Antibody [NBP3-35337] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TCP1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: TCP1 alpha Antibody [NBP3-35337] - Western blot analysis of various lysates using TCP1 alpha Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35337_rabbit-tcp1-alpha-pab-91220241519560.jpg)
![Immunohistochemistry: TCP1 alpha Antibody [NBP3-35337] - Immunohistochemistry analysis of paraffin-embedded Rat testis using TCP1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35337_rabbit-tcp1-alpha-pab-912202414213799.jpg)
![Western Blot: TCP1 alpha Antibody [NBP1-88149] - Analysis using Anti-TCP1 antibody NBP1-88149 (A) shows similar pattern to independent antibody NBP1-88148 (B).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NBP1-88149-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TCP1 alpha Antibody [NBP1-88149] - Analysis using Anti-TCP1 antibody NBP1-88149 (A) shows similar pattern to independent antibody NBP1-88148 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NBP1-88149] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-88149-img0001.jpg)
![Western Blot: TCP1 alpha Antibody [NBP1-88149] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-Western-Blot-NBP1-88149-img0004.jpg)
![Western Blot: TCP1 alpha Antibody (3G9G8) [NBP3-16290] - Western blot analysis of extracts of Jurkat cells, using TCP1 alpha antibody (NBP3-16290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-3G9G8-Western-Blot-NBP3-16290-img0002.jpg "Western Blot: TCP1 alpha Antibody (3G9G8) [NBP3-16290] - Western blot analysis of extracts of Jurkat cells, using TCP1 alpha antibody (NBP3-16290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: TCP1 alpha Antibody (3G9G8) [NBP3-16290] - Immunohistochemistry of paraffin-embedded human colon using TCP1 alpha Rabbit mAb (NBP3-16290) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TCP1-alpha-Antibody-3G9G8-Immunohistochemistry-Paraffin-NBP3-16290-img0001.jpg)
![Western Blot: TCP1 alpha Antibody (SR2043) [NBP3-22063] - Western blot analysis using NBP3-22063 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22063_rabbit-tcp1-alpha-mab-sr2043-5720231754827.jpg "Western Blot: TCP1 alpha Antibody (SR2043) [NBP3-22063] - Western blot analysis using NBP3-22063 in HeLa cell lysate.")
![Western Blot: TCP1 alpha Overexpression Lysate (Adult Normal) [NBP2-06194] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TCP1 alpha.](http://images.novusbio.com/fullsize/TCP1-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06194-img0001.jpg "Western Blot: TCP1 alpha Overexpression Lysate (Adult Normal) [NBP2-06194] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TCP1 alpha.")
![SDS-Page: Recombinant Human TCP1 alpha His Protein [NBP1-50854] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Human TCP1 alpha His Protein-SDS-Page-NBP1-50854-img0003.jpg "SDS-Page: Recombinant Human TCP1 alpha His Protein [NBP1-50854] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")