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![Western Blot: RGL2 Antibody (4D10) [H00005863-M02] - Impact of RGL2 depletion on RalB activation at autophagosomes upon starvation. Validation of RGL2 depletions. Representative western blots for RGL2 and GAPDH from cell lysates of HEK-HT cells prepared 72 hrs after transfection with the indicated siRNAs. Quantifications of RGL2 protein depletion, normalized for siControl condition (=100), are shown below the WB. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-45443-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RGL2-Antibody-4D10-Western-Blot-H00005863-M02-img0009.jpg "Western Blot: RGL2 Antibody (4D10) [H00005863-M02] - Impact of RGL2 depletion on RalB activation at autophagosomes upon starvation. Validation of RGL2 depletions. Representative western blots for RGL2 and GAPDH from cell lysates of HEK-HT cells prepared 72 hrs after transfection with the indicated siRNAs. Quantifications of RGL2 protein depletion, normalized for siControl condition (=100), are shown below the WB. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-45443-1), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: RGL2 Antibody (4D10) [H00005863-M02] - Analysis of monoclonal antibody to RGL2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/RGL2-Antibody-4D10-Immunocytochemistry-Immunofluorescence-H00005863-M02-img0005.jpg)
![Western Blot: RGL2 Antibody (4D10) [H00005863-M02] - RGL2 monoclonal antibody (M02), clone 4D10 Analysis of RGL2 expression in HeLa.](http://images.novusbio.com/fullsize/RGL2-Antibody-4D10-Western-Blot-H00005863-M02-img0007.jpg)
![ELISA: RGL2 Antibody (4D10) [H00005863-M02] - Detection limit for recombinant GST tagged RGL2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RGL2-Antibody-4D10-ELISA-H00005863-M02-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: RGL2 Antibody (4D10) [H00005863-M02] - Localization of RGL2 at different endomembrane compartments. HEK-HT (Normal cells) & HEK-HT-H-RasV12 (Transformed cells) cells were fixed & imaged for endogenous RGL2 (IF anti-RGL2, represented in red), together with EEA1 (early endosome marker, IF anti-EEA1, represented in green), GFP-Rab11 (recycling endosome marker, represented in green), GFP-Rab6 (Trans-Golgi marker, represented in green), or iRFP-LC3 (autophagosome marker, represented in green). Representative confocal cross sections of normal cells are shown (left). Quantifications are reported for both normal & transformed cells (right). Localization was calculated as percentage (%) of the indicated endomembrane compartments positive for RGL2, as compared to the control pseudo-compartments. Each dot corresponds to one cell. Graph represents mean ± SEM of 21 to 40 cells from 3–4 independent experiments. For statistics Mann Whitney test was used. **p value < 0.01, ***p value < 0.001. Scale bars are 10 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31222145), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005863-m02_mouse-monoclonal-rgl2-antibody-4d10-310202415482283.jpg)
![Western Blot: RGL2 Antibody (4D10) [H00005863-M02] - Impact of RGL2 depletion on RalB activation at autophagosomes upon starvation. (A) Modulation by autophagy of RalB activity at autophagosomes upon RGL2 depletion. Normal HEK-HT cells were transfected with indicated siRNAs (day 0), then transfected with FRET RalB biosensor & Cherry-LC3 plasmids (day 2), & incubated in either basal (DMEM) or starvation (EBSS) medium (4 hours) before imaging (day 3). Graph represents mean ± SEM of n = 17 to 57 cells per condition, from 3 independent experiments. To compare DMEM versus EBSS conditions Mann Whitney test was used, **p < 0.01, ***, & p < 0.001. (B) Validation of RGL2 depletions. Representative western blots for RGL2 & GAPDH from cell lysates of HEK-HT cells prepared 72 hrs after transfection with the indicated siRNAs. Quantifications of RGL2 protein depletion, normalized for siControl condition (=100), are shown below the WB. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31222145), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00005863-m02_mouse-monoclonal-rgl2-antibody-4d10-310202415485145.jpg)
![Western Blot: RGL2 Antibody (OTI10F2) [NBP2-45572] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGL2.](http://images.novusbio.com/fullsize/RGL2-Antibody-OTI10F2-Western-Blot-NBP2-45572-img0001.jpg "Western Blot: RGL2 Antibody (OTI10F2) [NBP2-45572] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGL2.")
![Immunohistochemistry-Paraffin: RGL2 Antibody (OTI10F2) [NBP2-45572] - Staining of paraffin-embedded Human lymphoma tissue using anti-RGL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGL2-Antibody-OTI10F2-Immunohistochemistry-Paraffin-NBP2-45572-img0006.jpg)
![Immunohistochemistry-Paraffin: RGL2 Antibody (OTI10F2) [NBP2-45572] - Staining of paraffin-embedded Human colon tissue using anti-RGL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGL2-Antibody-OTI10F2-Immunohistochemistry-Paraffin-NBP2-45572-img0002.jpg)
![Immunohistochemistry-Paraffin: RGL2 Antibody (OTI10F2) [NBP2-45572] - Staining of paraffin-embedded Human Kidney tissue using anti-RGL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGL2-Antibody-OTI10F2-Immunohistochemistry-Paraffin-NBP2-45572-img0003.jpg)
![Immunohistochemistry-Paraffin: RGL2 Antibody (OTI10F2) [NBP2-45572] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RGL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGL2-Antibody-OTI10F2-Immunohistochemistry-Paraffin-NBP2-45572-img0004.jpg)
![Immunohistochemistry-Paraffin: RGL2 Antibody (OTI10F2) [NBP2-45572] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RGL2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/RGL2-Antibody-OTI10F2-Immunohistochemistry-Paraffin-NBP2-45572-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RGL2 Antibody [NBP2-68676] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/RGL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68676-img0001.jpg "Immunocytochemistry/Immunofluorescence: RGL2 Antibody [NBP2-68676] - Staining of human cell line SK-MEL-30 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry: RGL2 Antibody [NBP2-30581] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/RGL2-Antibody-Immunohistochemistry-NBP2-30581-img0001.jpg "Immunohistochemistry: RGL2 Antibody [NBP2-30581] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Western Blot: RGL2 Overexpression Lysate (Adult Normal) [NBL1-15313] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RGL2.](http://images.novusbio.com/fullsize/RGL2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15313-img0002.jpg "Western Blot: RGL2 Overexpression Lysate (Adult Normal) [NBL1-15313] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RGL2.")
![Western Blot: RGL2 Antibody (4F1) [H00005863-M01] - Detection against Immunogen (36.63 KDa) .](http://images.novusbio.com/fullsize/RGL2-Antibody-4F1-Western-Blot-H00005863-M01-img0001.jpg "Western Blot: RGL2 Antibody (4F1) [H00005863-M01] - Detection against Immunogen (36.63 KDa) .")
