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![Western Blot: PHF1 Antibody [NBP1-82613] - Analysis in human testis tissue.](http://images.novusbio.com/fullsize/PHF1-Antibody-Western-Blot-NBP1-82613-img0009.jpg "Western Blot: PHF1 Antibody [NBP1-82613] - Analysis in human testis tissue.")
![Immunocytochemistry/Immunofluorescence: PHF1 Antibody [NBP1-82613] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82613-img0006.jpg)
![Immunohistochemistry: PHF1 Antibody [NBP1-82613] - Imaging PHF-1 expression in prostate cancer. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PHF1-Antibody-Immunohistochemistry-NBP1-82613-img0008.jpg)
![Immunohistochemistry-Paraffin: PHF1 Antibody [NBP1-82613] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PHF1-Antibody-Immunohistochemistry-Paraffin-NBP1-82613-img0007.jpg)
![Western Blot: PHF1 Antibody (2D3) [H00005252-M01] - PHF1 monoclonal antibody (M01), clone 2D3 Analysis of PHF1 expression in A-431.](http://images.novusbio.com/fullsize/PHF1-Antibody-2D3-Western-Blot-H00005252-M01-img0003.jpg "Western Blot: PHF1 Antibody (2D3) [H00005252-M01] - PHF1 monoclonal antibody (M01), clone 2D3 Analysis of PHF1 expression in A-431.")
![Western Blot: PHF1 Antibody (2G7) [H00005252-M04] - PHF1 monoclonal antibody (M04), clone 2G7. Western Blot analysis of PHF1 expression in Jurkat.](http://images.novusbio.com/fullsize/PHF1-Antibody-2G7-Western-Blot-H00005252-M04-img0007.jpg "Western Blot: PHF1 Antibody (2G7) [H00005252-M04] - PHF1 monoclonal antibody (M04), clone 2G7. Western Blot analysis of PHF1 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: PHF1 Antibody (2G7) [H00005252-M04] - Analysis of monoclonal antibody to PHF1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PHF1-Antibody-2G7-Immunocytochemistry-Immunofluorescence-H00005252-M04-img0005.jpg)
![Sandwich ELISA: PHF1 Antibody (2G7) [H00005252-M04] - Detection limit for recombinant GST tagged PHF1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHF1-Antibody-2G7-Sandwich-ELISA-H00005252-M04-img0006.jpg)
![Western Blot: PHF1 Antibody [NBP2-98955] - Anti-PHF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 67 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PHF1-Antibody-Western-Blot-NBP2-98955-img0003.jpg "Western Blot: PHF1 Antibody [NBP2-98955] - Anti-PHF1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: NCI-H1299 Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 67 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: PHF1 Antibody [NBP2-98955] - Immunofluorescence staining of PHF1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PHF1 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/PHF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98955-img0001.jpg)
![Western Blot: PHF1 Antibody [NBP3-38547] - Western blot analysis of various lysates using PHF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38547_rabbit-phf1-pab-101220241214432.jpg "Western Blot: PHF1 Antibody [NBP3-38547] - Western blot analysis of various lysates using PHF1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: PHF1 Antibody (4D8) [H00005252-M02] - PHF1 monoclonal antibody (M02), clone 4D8 Analysis of PHF1 expression in K-562.](http://images.novusbio.com/fullsize/PHF1-Antibody-4D8-Western-Blot-H00005252-M02-img0004.jpg "Western Blot: PHF1 Antibody (4D8) [H00005252-M02] - PHF1 monoclonal antibody (M02), clone 4D8 Analysis of PHF1 expression in K-562.")
![Sandwich ELISA: PHF1 Antibody (4D8) [H00005252-M02] - Detection limit for recombinant GST tagged PHF1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHF1-Antibody-4D8-Sandwich-ELISA-H00005252-M02-img0003.jpg)
![Western Blot: PHF1 Antibody (2A12) [H00005252-M03] - Analysis of PHF1 expression in K-562 (Cat # L009V1).](http://images.novusbio.com/fullsize/PHF1-Antibody-2A12-Western-Blot-H00005252-M03-img0003.jpg "Western Blot: PHF1 Antibody (2A12) [H00005252-M03] - Analysis of PHF1 expression in K-562 (Cat # L009V1).")
![ELISA: PHF1 Antibody (2A12) [H00005252-M03] - Detection limit for recombinant GST tagged PHF1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHF1-Antibody-2A12-ELISA-H00005252-M03-img0002.jpg)
![Western Blot: PHF1 Antibody [NBP1-32120] - Sample (30 ug of whole cell lysate)A: A431 B: H129910% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PHF1-Antibody-Western-Blot-NBP1-32120-img0002.jpg "Western Blot: PHF1 Antibody [NBP1-32120] - Sample (30 ug of whole cell lysate)A: A431 B: H129910% SDS PAGE, antibody diluted at 1:1000.")
![Western Blot: PHF1 Overexpression Lysate (Adult Normal) [NBP2-06342] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF1.](http://images.novusbio.com/fullsize/PHF1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06342-img0001.jpg "Western Blot: PHF1 Overexpression Lysate (Adult Normal) [NBP2-06342] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF1.")