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![Western Blot: preproANP Antibody [NBP2-14873] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/preproANP-Antibody-Western-Blot-NBP2-14873-img0025.jpg "Western Blot: preproANP Antibody [NBP2-14873] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [N1C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse muscle. ANP stained by ANP antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/preproANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14873-img0027.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: preproANP Antibody [NBP2-14873] - Knockdown of ATE1 attenuates cardiac hypertrophy. Western blot analysis of preproANP protein levels in heart samples from sham and renal ligated rats (B2) bar graph was generated by quantifying the blots and normalizing the intensities of bands to the untreated lane (C) BNP and (D) beta-MHC in Ligated ATE1 siRNA when compared with Ligated NS siRNA samples. Statistical analysis was carried out by one-way ANOVA. Data are represented as mean +/- SE. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57379-7) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Western-Blot-NBP2-14873-img0016.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: preproANP Antibody [NBP2-14873] - Knockdown of ATE1 attenuates cardiac hypertrophy. Western blot analysis of preproANP protein levels in heart samples from sham and renal ligated rats (B2) bar graph was generated by quantifying the blots and normalizing the intensities of bands to the untreated lane (C) BNP and (D) beta-MHC in Ligated ATE1 siRNA when compared with Ligated NS siRNA samples. Statistical analysis was carried out by one-way ANOVA. Data are represented as mean +/- SE. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-57379-7) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Western-Blot-NBP2-14873-img0017.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Human hepatoma, using ANP antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14873-img0005.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse muscle. ANP antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14873-img0009.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Mouse heart. ANP stained by preproANP antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14873-img0014.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - Rat heart. ANP stained by ANP antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/preproANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14873-img0026.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14873] - preproANP antibody [N1C3] detects secreted preproANP protein by immunohistochemical analysis.Sample: Paraffin-embedded rat heart.preproANP stained by preproANP antibody [N1C3] (NBP2-14873) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-14873_rabbit-polyclonal-preproanp-antibody-91020242041299.jpg)
![N/A Atrial Natriuretic Peptide/ANP [HRP]](http://images.novusbio.com/fullsize2/Atrial_Natriuretic_Peptide_DANP00_ELISA_1155.jpg "N/A Atrial Natriuretic Peptide/ANP [HRP]")
![N/A Atrial Natriuretic Peptide/ANP [HRP]](http://images.novusbio.com/fullsize2/Atrial_Natriuretic_Peptide_DANP00_ELISA_1154.jpg)
![Western Blot: preproANP Antibody [NBP2-14872] - Expression of cardiac hypertrophic biomarkers in cultured neonatal cardiomyocytes. Secretory preproANP was detected via immunoblot analyses of culture media collected at indicated time points and normalized to total protein measured by Coomassie staining (n = 4 mice). Pictured are representative scans of the preproANP immunoblot and corresponding Coomassie staining. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32377307/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/preproANP-Antibody-Western-Blot-NBP2-14872-img0010.jpg "Western Blot: preproANP Antibody [NBP2-14872] - Expression of cardiac hypertrophic biomarkers in cultured neonatal cardiomyocytes. Secretory preproANP was detected via immunoblot analyses of culture media collected at indicated time points and normalized to total protein measured by Coomassie staining (n = 4 mice). Pictured are representative scans of the preproANP immunoblot and corresponding Coomassie staining. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32377307/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14872] - Rat heart. ANP stained by preproANP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/preproANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14872-img0009.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14872] - Human ovarian carcinoma. preproANP antibody dilution: 1:250. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/preproANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14872-img0003.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-14872] - Mouse heart. ANP stained by preproANP antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/preproANP-Antibody-Immunohistochemistry-Paraffin-NBP2-14872-img0008.jpg)
![Western Blot: preproANP Antibody [NBP2-14872] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-14872_rabbit-polyclonal-preproanp-antibody-western-blot-2732024103018..jpg)
![N/A Atrial Natriuretic Peptide/ANP [Biotin]](http://images.novusbio.com/fullsize2/DATA_Atrial_Natriuretic_Peptide_DY824705_ELISA_2538.jpg "N/A Atrial Natriuretic Peptide/ANP [Biotin]")
![Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Analysis of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/proANP-Antibody-Western-Blot-NBP1-97752-img0001.jpg "Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Analysis of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/proANP-Antibody-Immunohistochemistry-Paraffin-NBP1-97752-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [1068] iluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-GT1068-Immunocytochemistry-Immunofluorescence-NBP3-13605-img0002.jpg "Immunocytochemistry/Immunofluorescence: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ANP stained by ANP antibody [1068] iluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000. Blue: Fluoroshield with DAPI. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse heart. ANP stained by ANP antibody [1068] diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-GT1068-Immunohistochemistry-Paraffin-NBP3-13605-img0003.jpg)
![Western Blot: Atrial Natriuretic Peptide/ANP Antibody (GT1068) [NBP3-13605] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Atrial Natriuretic Peptide/ANP antibody [GT1068] (NBP3-13605) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13605_mouse-atrial-natriuretic-peptide-anp-mab-gt1068-910202420131210.jpg)
![Immunohistochemistry: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35467] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Atrial Natriuretic Peptide/ANP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35467_rabbit-atrial-natriuretic-peptide-anp-pab-91220241515288.jpg "Immunohistochemistry: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35467] - Immunohistochemistry analysis of paraffin-embedded Rat heart using Atrial Natriuretic Peptide/ANP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35467] - Immunohistochemistry analysis of paraffin-embedded Mouse heart using Atrial Natriuretic Peptide/ANP Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35467_rabbit-atrial-natriuretic-peptide-anp-pab-912202415143665.jpg)
![Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP3-35467] - Western blot analysis of lysates from Mouse heart, using Atrial Natriuretic Peptide/ANP Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35467_rabbit-atrial-natriuretic-peptide-anp-pab-9122024159534.jpg)
![Western Blot: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NPPA using NPPA Rabbit mAb at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33325_rabbit-atrial-natriuretic-peptide-anp-mab-2c5s5-10122024140453.jpg "Western Blot: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NPPA using NPPA Rabbit mAb at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.")
![Immunohistochemistry: Atrial Natriuretic Peptide/ANP Antibody (2C5S5) [NBP3-33325] - Confocal imaging of paraffin-embedded mouse heart using NPPA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L)(Red). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-33325_rabbit-atrial-natriuretic-peptide-anp-mab-2c5s5-10122024140419.jpg)
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-34105] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP2-34105-img0003.jpg "Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-34105] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-34105] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP2-34105-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: preproANP Antibody [NBP2-34105] - Staining in human heart muscle and skeletal muscle tissues using anti-preproANP antibody. Corresponding preproANP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP2-34105-img0004.jpg)
![Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP3-17073] - Analysis in human heart muscle and duodenum tissues using Anti-NPPA antibody. Corresponding NPPA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP3-17073-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP3-17073] - Analysis in human heart muscle and duodenum tissues using Anti-NPPA antibody. Corresponding NPPA RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP3-17073] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP3-17073-img0002.jpg)
![Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP3-17073] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Atrial-Natriuretic-Peptide-ANP-Antibody-Immunohistochemistry-Paraffin-NBP3-17073-img0003.jpg)
![ELISA: Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) [NBP2-66733] - Samples were spiked with high concentrations of Mouse Atrial Natriuretic Peptide/ANP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66733_mouse-atrial-natriuretic-peptide-anp-elisa-kit-color-132202416225297.jpg "ELISA: Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) [NBP2-66733] - Samples were spiked with high concentrations of Mouse Atrial Natriuretic Peptide/ANP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Atrial Natriuretic Peptide/ANP ELISA Kit (Colorimetric) [NBP2-66733] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66733_mouse-atrial-natriuretic-peptide-anp-elisa-kit-color-432024856775.jpg)