| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3635-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23602SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3526-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB35261-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | MAB35262-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05613-20ul | |||
![Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).](http://images.novusbio.com/fullsize/GLI-2-Antibody-Western-Blot-NB600-874-img0012.jpg "Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NB600-874] - Mouse adenocarcinoma section shows high and diffuse nuclear Gli2 expression. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NB600-874-img0013.jpg)
![Flow (Intracellular): GLI-2 Antibody [NB600-874] - intracellular staining for GLI-2 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-2 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Flow-Intracellular-NB600-874-img0006.jpg)
![Immunohistochemistry: GLI-2 Antibody [NB600-874] - Staining of tumor cells in human breast tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NB600-874-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0007.jpg "Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with GLI-2 Antibody (NBP2-23602) at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: GLI-2 Antibody [NBP2-23602] - GLI-2 (red) and DAPI (blue) in mouse BT-474 primary tumors (breast cancer tissue). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-Paraffin-NBP2-23602-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - GLI-2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-23602-img0004.jpg)
![Immunohistochemistry: GLI-2 Antibody [NBP2-23602] - Analysis of GLI-2 in mouse heart.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunohistochemistry-NBP2-23602-img0005.jpg)
![Immunohistochemistry-Paraffin: GLI-2 Antibody (524409) [Unconjugated] [MAB35262] - GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35262) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/GLI-2-Antibody-524409-Unconjugated-Immunohistochemistry-Paraffin-MAB35262-img0001.jpg "Immunohistochemistry-Paraffin: GLI-2 Antibody (524409) [Unconjugated] [MAB35262] - GLI-2 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human GLI-2 Monoclonal Antibody (Catalog # MAB35262) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Western Blot: GLI-2 Antibody [NBP3-05613] - Western blot analysis of extracts of various cell lines, using GLI-2 antibody (NBP3-05613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Western-Blot-NBP3-05613-img0001.jpg "Western Blot: GLI-2 Antibody [NBP3-05613] - Western blot analysis of extracts of various cell lines, using GLI-2 antibody (NBP3-05613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-57535] - Staining of human cell line A549 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GLI-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57535-img0001.jpg "Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-57535] - Staining of human cell line A549 shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Western Blot: Gli2 Overexpression Lysate (Adult Normal) [NBP2-07768] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Gli2.](http://images.novusbio.com/fullsize/Gli2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07768-img0001.jpg "Western Blot: Gli2 Overexpression Lysate (Adult Normal) [NBP2-07768] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Gli2.")
![Immunoprecipitation:GLI-2 AntibodyNBP3-29457] -Detection of human GLI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLI2 antibody NBP3-29457 (lot NBP3-29457-1) used for IP at 6 µg per reaction. GLI2 was also immunoprecipitated by rabbit anti-GLI2 antibodies BL20733 and BL20735. For blotting immunoprecipitated GLI2, NBP3-29457 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29457_rabbit-gli-2-pab-301220241652440.jpg "Immunoprecipitation:GLI-2 AntibodyNBP3-29457] -Detection of human GLI2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GLI2 antibody NBP3-29457 (lot NBP3-29457-1) used for IP at 6 µg per reaction. GLI2 was also immunoprecipitated by rabbit anti-GLI2 antibodies BL20733 and BL20735. For blotting immunoprecipitated GLI2, NBP3-29457 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")