| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31147SS | |||
| Azide and BSA Free | NBP2-80887 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31146SS | |||
| Azide and BSA Free | NBP2-80886 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31147SS | |||
| BSA Free | NBP2-31147 | |||
| Azide and BSA Free | NBP2-80887 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31147SS | |||
| BSA Free | NBP2-31147 | |||
| Azide and BSA Free | NBP2-80887 | |||
![Western Blot: NUT Antibody (46B4G9.2B6) [NBP2-31147] - WB analysis of partial recombinant NUT protein (expected molecular weight ~17 kDa) with NUT antibody (clone 46B4G9.2B6) at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/NUT-Antibody-46B4G9-2B6-Western-Blot-NBP2-31147-img0001.jpg "Western Blot: NUT Antibody (46B4G9.2B6) [NBP2-31147] - WB analysis of partial recombinant NUT protein (expected molecular weight ~17 kDa) with NUT antibody (clone 46B4G9.2B6) at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: NUT Antibody (46B4G9.2B6) [NBP2-31147] - NUT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.01 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/NUT-Antibody-46B4G9-2B6-Immunocytochemistry-Immunofluorescence-NBP2-31147-img0009.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody (46B4G9.2B6) [NBP2-31147] - IHC-P analysis of NUT protein in normal human skin tissue section using NUT antibody (clone 46B4G9.2B6) at a concentration of 5 ug/ml. This antibody stained the nuclei and cytoplasm of different epidermal cell types such as granulosa cells, keratinocytes, Langerhans' cell and pigmented cells, and dermal blood vessels.](http://images.novusbio.com/fullsize/NUT-Antibody-46B4G9-2B6-Immunohistochemistry-Paraffin-NBP2-31147-img0002.jpg)
![Western Blot: NUT Antibody (46B3D2.1G6) [NBP2-31146] - WB analysis of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein (expected molecular weight ~17kda) with NUT antibody (clone 46B3D2.1G6) at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 at ~120 kDa and ~46kDa respectively.](http://images.novusbio.com/fullsize/NUT-Antibody-46B3D2-1G6-Western-Blot-NBP2-31146-img0002.jpg "Western Blot: NUT Antibody (46B3D2.1G6) [NBP2-31146] - WB analysis of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein (expected molecular weight ~17kda) with NUT antibody (clone 46B3D2.1G6) at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 at ~120 kDa and ~46kDa respectively.")
![Immunocytochemistry/Immunofluorescence: NUT Antibody (46B3D2.1G6) [NBP2-31146] - NUT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/NUT-Antibody-46B3D2-1G6-Immunocytochemistry-Immunofluorescence-NBP2-31146-img0006.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody (46B3D2.1G6) [NBP2-31146] - IHC-P analysis of NUT protein in normal human skin tissue section using NUT antibody (clone 46B3D2.1G6) at a concentration of 5 ug/ml. This antibody stained the nuclei and cytoplasm of different epidermal cell types such as granulosa cells, keratinocytes, Langerhans' cell and pigmented cells, and dermal blood vessels.](http://images.novusbio.com/fullsize/NUT-Antibody-46B3D2-1G6-Immunohistochemistry-Paraffin-NBP2-31146-img0003.jpg)
![Western Blot: NUT Antibody [NBP2-31370] - WB detection of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein with NUT antibody at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 of NUT protein at ~120 kDa and ~46kDa respectively.](http://images.novusbio.com/fullsize/NUT-Antibody-Western-Blot-NBP2-31370-img0001.jpg "Western Blot: NUT Antibody [NBP2-31370] - WB detection of NUT/ NUTM1 protein in (A) human testis lysate and on (B) partial recombinant NUT protein with NUT antibody at a concentration of 2 ug/ml. In human testis, this antibody detected Isoform 1 and Isoform 2 of NUT protein at ~120 kDa and ~46kDa respectively.")
![Immunocytochemistry/Immunofluorescence: NUT Antibody [NBP2-31370] - NUT antibody was tested at 0.01 ug/ml in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31370-img0005.jpg)
![Western Blot: NUT Antibody (PSH02-78) [NBP3-32673] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32673) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32673) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-278202415242684.jpg "Western Blot: NUT Antibody (PSH02-78) [NBP3-32673] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32673) at 1/2,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 4 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32673) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32673) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32673) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-298202413585581.jpg)
![Immunohistochemistry: NUT Antibody (PSH02-78) [NBP3-32673] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32673) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32673, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-298202413585547.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody (PSH02-78) [NBP3-32673] - Immunocytochemistry analysis of 293T transfacted with or without mouse NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32673) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32673) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32673_rabbit-nut-mab-psh02-78-2920241192316.jpg)
![Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-278202416331244.jpg "Western Blot: NUT Antibody [NBP3-32672] - Western blot analysis of NUT on mouse testis tissue lysates with Rabbit anti-NUT antibody (NBP3-32672) at 1/2,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 120 kDa Observed band size: 120 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32672) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32672) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32672) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-29820241411542.jpg)
![Immunohistochemistry: NUT Antibody [NBP3-32672] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling NUT with Rabbit anti-NUT antibody (NBP3-32672) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32672, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-298202414115413.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody [NBP3-32672] - Immunocytochemistry analysis of 293T transfacted with or without mouse NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32672) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32672) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32672_rabbit-nut-mab-29202410484895.jpg)
![Western Blot: NUT Antibody (PSH03-37) [NBP3-32675] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32675) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-hNutm1 cell lysate Lysates/proteins at 20 ug/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32675) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32675_rabbit-nut-mab-psh03-37-2882024142266.jpg "Western Blot: NUT Antibody (PSH03-37) [NBP3-32675] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32675) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-hNutm1 cell lysate Lysates/proteins at 20 ug/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32675) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody (PSH03-37) [NBP3-32675] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32675) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32675) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32675_rabbit-nut-mab-psh03-37-29820241358552.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody (PSH03-37) [NBP3-32675] - Immunocytochemistry analysis of HeLa transfacted with or without human NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32675) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32675) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32675_rabbit-nut-mab-psh03-37-2920241192399.jpg)
![Western Blot: NUT Antibody (PSH03-36) [NBP3-32674] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32674) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-hNutm1 cell lysate Lysates/proteins at 20 ug/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32674) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32674_rabbit-nut-mab-psh03-36-27820241631613.jpg "Western Blot: NUT Antibody (PSH03-36) [NBP3-32674] - Western blot analysis of NUT on different lysates with Rabbit anti-NUT antibody (NBP3-32674) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-hNutm1 cell lysate Lysates/proteins at 20 ug/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32674) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NUT Antibody (PSH03-36) [NBP3-32674] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NUT antibody (NBP3-32674) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32674) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32674_rabbit-nut-mab-psh03-36-29820241420983.jpg)
![Immunocytochemistry/ Immunofluorescence: NUT Antibody (PSH03-36) [NBP3-32674] - Immunocytochemistry analysis of HeLa transfacted with or without human NUT cells labeling Nutm1 with Rabbit anti-NUT antibody (NBP3-32674) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NUT antibody (NBP3-32674) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32674_rabbit-nut-mab-psh03-36-2920241192336.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody [NBP1-84176] - Analysis in human testis and endometrium tissues. Corresponding NUTM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunohistochemistry-Paraffin-NBP1-84176-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NUT Antibody [NBP1-84176] - Analysis in human testis and endometrium tissues. Corresponding NUTM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NUT Antibody [NBP1-84176] - Staining of human testis shows weak to moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunohistochemistry-Paraffin-NBP1-84176-img0014.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody [NBP1-84176] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunohistochemistry-Paraffin-NBP1-84176-img0012.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody [NBP1-84176] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunohistochemistry-Paraffin-NBP1-84176-img0013.jpg)
![Immunohistochemistry-Paraffin: NUT Antibody [NBP1-84176] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NUT-Antibody-Immunohistochemistry-Paraffin-NBP1-84176-img0015.jpg)