Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Ha,
Pm,
Rb,
Sh,
YeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Ha, Pm, Rb, Sh, Ye
WB, ICC/IF, IHC, WB
Applications: WB, ICC/IF, IHC, WB
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment and validation of tagged exocyst subunit cell lines. Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, and SEC8), or in one allele (SEC3 and SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-07467-5) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SEC6-Antibody-9H5-Western-Blot-NBP1-97500-img0005.jpg "Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment and validation of tagged exocyst subunit cell lines. Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, and SEC8), or in one allele (SEC3 and SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-07467-5) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of human tonsil tissue stained with rSec6, mAb (9H5) at 10ug/ml.](http://images.novusbio.com/fullsize/SEC6-Antibody-9H5-Immunohistochemistry-Paraffin-NBP1-97500-img0003.jpg)
![Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Analysis of pig ESK-4 cell lysate.](http://images.novusbio.com/fullsize/SEC6-Antibody-9H5-Western-Blot-NBP1-97500-img0004.jpg)
![Western Blot: SEC6 Antibody (9H5) [NBP1-97500] - Establishment & validation of tagged exocyst subunit cell lines. a Schematic showing a region of subunit gene targeted by sgRNA & tags to be inserted C-terminally, in-frame with the coding region. b Strategy to isolate CRISPR/Cas9-mediated sfGFP-tagged clones of exocyst subunits in NMuMG cells. c Western blots with subunit specific antibodies show successful incorporation of sfGFP in both alleles (EXO70, SEC5, & SEC8), or in one allele (SEC3 & SEC6). A shRNA specific to SEC3 was used to confirm the identity of multiple bands in the blot. d Confocal images of live wild-type NMuMG cells & endogenously tagged SEC3-GFP, SEC5-GFP, SEC6-GFP, SEC8-GFP, & EXO70-GFP cell lines. Scale bar = 10 μm. e Protein–protein interaction heat map for endogenous SEC3-GFP, SEC5-GFP, & EXO70-GFP pulldown using GFP-Trap nanobodies & MS. Yellow indicates baits, blue the protein identified with high confidence, & gray denotes preys that were not identified. The experiments were repeated three times for EXO70-GFP & twice for SEC3-GFP & SEC5-GFP. All experiments were also partially confirmed by IP–WB in at least three independent experiments, with similar results. f Western blot analysis to assess the relative abundance of exocyst subunits fused to sfGFP, using anti-GFP antibodies (SEC3 & SEC6 quantifications corrected for heterozygosity). GAPDH was the loading control. Y axis shows the ratio of GFP/GAPDH. Quantification shows pooled data from three experiments Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30510181), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97500_mouse-monoclonal-sec6-antibody-9h5-310202416114734.jpg)
![Western Blot: SEC6 Antibody (4A7) [H00011336-M01] - Analysis of EXOC3 expression in transfected 293T cell line by EXOC3 monoclonal antibody (M01), clone 4A7.Lane 1: EXOC3 transfected lysate(86.845 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SEC6-Antibody-4A7-Western-Blot-H00011336-M01-img0005.jpg "Western Blot: SEC6 Antibody (4A7) [H00011336-M01] - Analysis of EXOC3 expression in transfected 293T cell line by EXOC3 monoclonal antibody (M01), clone 4A7.Lane 1: EXOC3 transfected lysate(86.845 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: SEC6 Antibody (4A7) [H00011336-M01] - Detection limit for recombinant GST tagged EXOC3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SEC6-Antibody-4A7-Sandwich-ELISA-H00011336-M01-img0004.jpg)
![Western Blot: SEC6 Antibody [NBP2-20282] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/SEC6-Antibody-Western-Blot-NBP2-20282-img0001.jpg "Western Blot: SEC6 Antibody [NBP2-20282] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: SEC6 Antibody [NBP2-20282] - Paraffin-embedded mouse middle brain. SEC6 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SEC6-Antibody-Immunohistochemistry-Paraffin-NBP2-20282-img0003.jpg)
![Immunohistochemistry-Paraffin: SEC6 Antibody [NBP2-20282] - Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SEC6-Antibody-Immunohistochemistry-Paraffin-NBP2-20282-img0002.jpg)
![Western Blot: SEC6 Antibody (SR2230) [NBP3-21986] - Western blot analysis using NBP3-21986 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21986_rabbit-sec6-mab-sr2230-6720239533723.jpg "Western Blot: SEC6 Antibody (SR2230) [NBP3-21986] - Western blot analysis using NBP3-21986 in MCF7 cell lysate.")
![Western Blot: SEC6 Antibody [NBP2-55179] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/SEC6-Antibody-Western-Blot-NBP2-55179-img0002.jpg "Western Blot: SEC6 Antibody [NBP2-55179] - Western blot analysis in human cell line RT-4.")
![Immunocytochemistry/Immunofluorescence: SEC6 Antibody [NBP2-55179] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize/SEC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55179-img0001.jpg)
![Western Blot: SEC6 Antibody [NBP1-55109] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.](http://images.novusbio.com/fullsize/SEC6-Antibody-Western-Blot-NBP1-55109-img0003.jpg "Western Blot: SEC6 Antibody [NBP1-55109] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.")
![Immunohistochemistry-Paraffin: SEC6 Antibody [NBP1-55109] - Formalin Fixed Paraffin; Embedded Tissue: Human Lung Tissue; Observed Staining: Cytoplasmic and membrane in alveolar type I cells; Primary Antibody Concentration: 1:100](http://images.novusbio.com/fullsize/SEC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-55109-img0004.jpg)
![Western Blot: SEC6 Antibody (1Z3J5) [NBP3-33237] - Western blot analysis of various lysates using SEC6 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33237_rabbit-sec6-mab-1z3j5-912202415161836.jpg "Western Blot: SEC6 Antibody (1Z3J5) [NBP3-33237] - Western blot analysis of various lysates using SEC6 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: SEC6 Antibody [NBP3-35769] - Western blot analysis of various lysates using SEC6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.](http://images.novusbio.com/fullsize/nbp3-35769_rabbit-sec6-pab-912202415133443.jpg "Western Blot: SEC6 Antibody [NBP3-35769] - Western blot analysis of various lysates using SEC6 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.")
![Western Blot: SEC6 Overexpression Lysate (Adult Normal) [NBL1-10378] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEC6.](http://images.novusbio.com/fullsize/SEC6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10378-img0002.jpg "Western Blot: SEC6 Overexpression Lysate (Adult Normal) [NBL1-10378] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEC6.")