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![Immunohistochemistry: USP18 Antibody [NBP3-35697] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using USP18 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35697_rabbit-usp18-pab-1012202411572674.jpg "Immunohistochemistry: USP18 Antibody [NBP3-35697] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using USP18 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: USP18 Antibody [NBP3-35697] - Western blot analysis of various lysates using USP18 Rabbit pAb at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35697_rabbit-usp18-pab-101220241224144.jpg)
![Immunocytochemistry/ Immunofluorescence: USP18 Antibody [NBP3-35697] - Immunofluorescence analysis of MCF7 cells using USP18 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35697_rabbit-usp18-pab-101220241243960.jpg)
![Immunohistochemistry: USP18 Antibody [NBP3-35697] - Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using USP18 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35697_rabbit-usp18-pab-101220241224159.jpg)
![Immunohistochemistry: USP18 Antibody [NBP3-35697] - Immunohistochemistry analysis of paraffin-embedded Human colon using USP18 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35697_rabbit-usp18-pab-1012202411572649.jpg)
![Immunohistochemistry: USP18 Antibody [NBP3-35697] - Immunohistochemistry analysis of paraffin-embedded Rat brain using USP18 Rabbit pAb at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35697_rabbit-usp18-pab-1012202411545541.jpg)
![Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.](http://images.novusbio.com/fullsize/USP18-Antibody-Western-Blot-NB600-893-img0004.jpg "Western Blot: USP18 Antibody [NB600-893] - Analysis of a band 43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3, Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is expected to be UBP43. The other bands are probably non-specific.")
![Western Blot: USP18 Antibody [H00011274-B02P] - Analysis of USP18 expression in transfected 293T cell line by USP18 polyclonal antibody. Lane 1: USP18 transfected lysate(40.92 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP18-Antibody-Western-Blot-H00011274-B02P-img0003.jpg "Western Blot: USP18 Antibody [H00011274-B02P] - Analysis of USP18 expression in transfected 293T cell line by USP18 polyclonal antibody. Lane 1: USP18 transfected lysate(40.92 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: USP18 Antibody [H00011274-B02P] - Analysis of purified antibody to USP18 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/USP18-Antibody-Immunocytochemistry-Immunofluorescence-H00011274-B02P-img0002.jpg)
![Western Blot: USP18 Overexpression Lysate (Adult Normal) [NBL1-17648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP18.](http://images.novusbio.com/fullsize/USP18-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17648-img0002.jpg "Western Blot: USP18 Overexpression Lysate (Adult Normal) [NBL1-17648] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for USP18.")
![Western Blot: USP18 Antibody [H00011274-B03P] - Analysis of USP18 expression in transfected 293T cell line. Lane 1: USP18 transfected lysate (40.92 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP18-Antibody-Western-Blot-H00011274-B03P-img0002.jpg "Western Blot: USP18 Antibody [H00011274-B03P] - Analysis of USP18 expression in transfected 293T cell line. Lane 1: USP18 transfected lysate (40.92 KDa). Lane 2: Non-transfected lysate.")