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![Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Western-Blot-NB600-412-img0007.jpg "Western Blot: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K golgi protein expression in rat liver tissue using NB600-412.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - HeLa cells, cultured on cover slips, were fixed with 10% formalin for 10 minutes and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were then incubated with 1:200 dilution of anti-58K Golgi Protein antibody (clone 58K-9) for overnight at 4C and detected with an anti-mouse Dylight 488 (Green) secondary at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at 1:500 dilution. Nuclei were counterstained with DAPI solution (Blue) [NBP2-31156]. Cells were imaged using a 40X objective. Antibody clone 58K-9 generated a specific signal in the Golgi apparatus of the cells.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunocytochemistry-Immunofluorescence-NB600-412-img0010.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - DMD muscle co-stained for Neu5Gc (green) with 58K Golgi, a Golgi marker, in red, and DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc and 58K Golgi. Bar is 50 um. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0088226) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0011.jpg)
![Immunohistochemistry: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Analysis of 58K Golgi Protein in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-58K-9-Immunohistochemistry-NB600-412-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Assessing Golgi morphology observed apoptosis in mBECs using Mouse 58K Golgi Protein at a dilution of 1:100 overnight at 4 °C. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-immunocytochemistry-immunofluorescence-204202310243.jpg)
![Immunocytochemistry/ Immunofluorescence: 58K Golgi Protein Antibody (58K-9) [NB600-412] - Co-localization of Neu5Gc staining with markers for endosomes & Golgi in BMD & DMD muscle.(A) BMD muscle co-stained for Neu5Gc (green) & clathrin (red), a marker of endosomes. Merged image on right shows overlap of Neu5Gc & clathrin expression in yellow. Arrow marks several examples of co-staining. (B) DMD muscle co-stained for Neu5Gc (green) with LAMP1, a lysosomal marker, 58K Golgi, a Golgi marker, or calnexin, & endoplasmic reticulum marker, all in red, & DAPI (blue). Arrow marks region of coincident staining (yellow) for Neu5Gc & 58K Golgi. Bar is 50 µm for all panels in A & B. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24505439), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-412_mouse-monoclonal-58k-golgi-protein-antibody-58k-9-310202415304219.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human cerebral cortex, colon, kidney and liver using Anti-FTCD antibody NBP2-48601 (A) shows similar protein distribution across tissues to independent antibody NBP2-48600 (B).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: 58K Golgi Protein Antibody [NBP2-48601] - Analysis using Anti-FTCD antibody NBP2-48601 (A) shows similar pattern to independent antibody NBP2-48600 (B).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NBP2-48601-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48601-img0008.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human colon.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0013.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0005.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0006.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0011.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human kidney.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0012.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48601] - Staining of human liver using Anti-FTCD antibody NBP2-48601.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48601-img0014.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human cerebral cortex, colon, kidney and liver using Anti-FTCD antibody NBP2-48651 (A) shows similar protein distribution across tissues to independent antibody NBP2-48600 (B).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: 58K Golgi Protein Antibody [NBP2-48651] - Analysis using Anti-FTCD antibody NBP2-48651 (A) shows similar pattern to independent antibody NBP2-48600 (B).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NBP2-48651-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48651-img0008.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human kidney.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0013.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0006.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0011.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human colon.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0012.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48651] - Staining of human liver using Anti-FTCD antibody NBP2-48651.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48651-img0014.jpg)
![Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NB100-1448-img0007.jpg "Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1448-img0009.jpg)
![Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Flow-Cytometry-NB100-1448-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1448-img0008.jpg)
![Western Blot: 58K Golgi Protein Antibody [NBP2-48600] - Analysis using Anti-FTCD antibody NBP2-48600 (A) shows similar pattern to independent antibody NBP2-48601 (B).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NBP2-48600-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: 58K Golgi Protein Antibody [NBP2-48600] - Analysis using Anti-FTCD antibody NBP2-48600 (A) shows similar pattern to independent antibody NBP2-48601 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0009.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining of human cerebral cortex, colon, kidney and liver using Anti-FTCD antibody NBP2-48600 (A) shows similar protein distribution across tissues to independent antibody NBP2-48601 (B).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0005.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining of human kidney.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0007.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining of human colon.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0008.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP2-48600] - Staining of human liver using Anti-FTCD antibody NBP2-48600.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP2-48600-img0010.jpg)
![Western Blot: 58K Golgi Protein Antibody [NBP1-32793] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NBP1-32793-img0004.jpg "Western Blot: 58K Golgi Protein Antibody [NBP1-32793] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP1-32793] - Human hepatocellular carcinoma. FTCD antibody [N1N3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-32793-img0005.jpg)
![Western Blot: 58K Golgi Protein Antibody [NBP1-32793] - Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NBP1-32793-img0001.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP1-86031] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86031-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP1-86031] - Staining in human liver and pancreas tissues using anti-FTCD antibody. Corresponding FTCD RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP1-86031] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86031-img0007.jpg)
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP1-86031] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-86031-img0006.jpg)
![Western Blot: 58K Golgi Protein Antibody [NBP1-54997] - Titration: 1.25ug/ml Positive Control: Fetal liver cell lysate.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NBP1-54997-img0004.jpg "Western Blot: 58K Golgi Protein Antibody [NBP1-54997] - Titration: 1.25ug/ml Positive Control: Fetal liver cell lysate.")
![Immunohistochemistry-Paraffin: 58K Golgi Protein Antibody [NBP1-54997] - Human Skin Tissue Squamous epithelial cells (Indicated with Arrows), 4-8ug/ml.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunohistochemistry-Paraffin-NBP1-54997-img0003.jpg)
![Western Blot: 58K Golgi Protein Overexpression Lysate (Adult Normal) [NBP2-07596] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 58K Golgi Protein.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07596-img0001.jpg "Western Blot: 58K Golgi Protein Overexpression Lysate (Adult Normal) [NBP2-07596] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 58K Golgi Protein.")
![Western Blot: 58K Golgi Protein Overexpression Lysate (Adult Normal) [NBP2-10129] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 58K Golgi Protein.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10129-img0001.jpg "Western Blot: 58K Golgi Protein Overexpression Lysate (Adult Normal) [NBP2-10129] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for 58K Golgi Protein.")