Autophagy is an essential process that maintains cellular homeostasis and carries out lysosome-mediated degradation of unwanted proteins in the cytoplasm. Because of this regulatory function, autophagy is often examined when looking at disease pathways. While our immune system initiates the removal of viruses and pathogens through the autophagic pathway, viruses, such as HIV, have developed a way to evade this process through inhibition. Therefore, developing a reliable way to examine the molecular process of this inhibition and interaction is very desired. The central autophagy