CRISPR-Cas9

By Victoria Osinski

The CRISPR-Cas9 genome-engineering tool is a powerful opportunity for researchers to study individual gene function. CRISPR-Cas9, abbreviated for Clustered Regularly Interspaced Short Palindromic Repeats, is a bacterial defense system that can be reprogrammed to target specific areas of DNA followed by precise editing.  Essentially, CRISPR sequences are transcribed into short RNA sequences that will match the desired DNA sequence of interest.

Why- If the CRISPR-Cas9 transfection is not successful, it would not be relevant to relate the observations from transfected cells to the expected outcome of gene editing experiment.

"CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism.  Specifically, the CRISPR sequence has been identified as a single DNA sequence that is repeated with unique sequences (found to be that of viruses) in-between.  Thus, bacteria have created an environment that allows them to recognize and attack viruses in case of a re-invasion.  "Cas", or CRISPR-associate protein, is the second part of this defense mechanism, and is responsible for cutting t

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Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funders, publishers, academics, pharma and antibody manufacturers were in attendance, to share and discuss best practices in research antibody validation and to drive improved antibody validation standards for the global life science community.

Telomeres are a region of repeat nucleotide sequences located at the end of chromosomes to protect our DNA from becoming damaged via end-to-end fusion.  TERF2, or telomeric-repeat binding factor 2, is important for telomere integrity and aids in the formation of the telosome, the telomeric loop, and control of the amount of DNA needed for telomere replication.