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Glypican 3 as a biomarker for gastro-esophageal adenocarcinoma

Expression of CD81 was detected by immunofluorescence with a mouse monoclonal antibody followed by secondary antibody labeled with DyLight488 and Phalloidin 568 staining for actin visualization

By Jamshed Arslan, Pharm. D., PhD.

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Exosome Markers

Carcinoembryonic antigen

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Isolating Immune Cells From Peripheral Blood: How and When to Use Density Gradient Centrifugation

Adaptive immunity is mediated by lymphocytes (e.g., T cells and B cells) while innate immunity responses are mediated by several cellular mediators (e.g., Neutrophils, Natural Killer Cells and Eosinophils)

By Victoria Osinski

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Immune cell markers

Victoria Osinski

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Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation

Expression of RhoA, B and C detected in the cytoplasm of A431 cells, a model of human epidermoid carcinoma, compared to negative control analyzed in the absence of RhoA Antibody (1A11-4G10) [NBP2-22528]

By Jamshed Arslan, Pharm. D., PhD.

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Tips to Optimize Your Western Blot for Phosphorylated Protein Detection

Western blotting allows identifying and quantifying proteins from cell lysates for determination of protein expression, sub-cellular localization, post-translational modifications, protein processing, and protein-protein interactions.

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Secondary antibodies

Phosphorylated proteins

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The Ins and Outs of Survivin

Exosomes arise from the late endosomal compartment through inward budding of multivesicular bodies and may contain multiple proteins, lipids, DNA, RNAs and biomolecules of viruses/prions.

By Rachel M.A. Linger, Ph.D.

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Cancer Research

ednrb-endothelin-r-type-b

Rachel M.A. Linger

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Explore IHC basics with this new guide: Immunohistochemistry Essential Elements and Beyond

3-color fluorescence IHC, nuclei-blue, cytoplasm-red and cytoskeleton-green, Immunohistochemistry Essential Elements and Beyond, Springer book

By Rosa Moreno, PhD.

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Immunohistochemistry

Primary antibodies

Secondary antibodies

Monoclonal Antibodies

Polyclonal antibodies

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Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation

Expression of DNMT2 in human colon tissue shows cytoplasmic and nuclear staining in glandular cells, IHC

By Jamshed Arslan, Pharm. D., PhD.

Tags:

Cancer Research

Immunohistochemistry

prostasin-prss8

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients

GFAP-green and Neurofilament-red expression in astrocytes of mouse brain cerebellum, IHC

By Michalina Hanzel, PhD

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Alexander disease

Rosenthal fibers

alphab-crystallin-cryab

Michalina Hanzel

Considerations for Quantitative Western blotting

GAPDH expression in human nucleus pulposus cell lysate, WB

By Jamshed Arslan, Pharm. D., PhD.

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Antibody treatment can generate microglia-like cells from bone marrow

By Jennifer Sokolowski, MD, PhD.

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