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Rat Laminin S/Laminin beta 2 ELISA Kit

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Summary
Product Discontinued
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    • Catalog Number
      BEK-2055-2P
    • Availability
      Product Discontinued

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Rat Laminin S/Laminin beta 2 ELISA Kit Summary

Immunogen
Laminin is a large (900 kDa) complex extracellular glycoprotein and a major component of the basement membrane. Laminin is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin is composed of three different polypeptide chains; alpha, beta and gamma which are bound to each other by disulphide bonds into a cross-shaped molecule. Isoforms have been detected for all three chains. The different combinations of the alpha, beta and gamma chains give rise to at least 15 laminin heterotrimers.
Marker
Schwann Cell Marker
Specificity
Rat Laminin
Kit Type
ELISA Kit
Gene
LAMB2

Applications/Dilutions

Dilutions
  • ELISA 156pg/ml-10000pg/ml
Application Notes
This product is useful for Sandwich ELISA detection. Strip format Biosensis ELISA kits offer the highest level of sensitivity available Strip 96-well format, use only what you need: High sensitivity and low blank Suitability for serum & tissues Reproducible results This kit contains: Pre-coated plates, Prepared standards Buffers, Detection reagents, Substrate Precise methods/instructions.

Reactivity Notes

Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Notes

This kit contains 2.0 x 96.0 Well Strip.

Alternate Names for Rat Laminin S/Laminin beta 2 ELISA Kit

  • LAMB2
  • Laminin B1s chain
  • Laminin beta 2
  • Laminin S
  • laminin subunit beta-2
  • laminin, beta 2 (laminin S)
  • Laminin-11 subunit beta
  • Laminin-14 subunit beta
  • Laminin-15 subunit beta
  • Laminin-3 subunit beta
  • Laminin-4 subunit beta
  • Laminin-7 subunit beta
  • LAMSLaminin-9 subunit beta
  • S-LAM beta
  • S-laminin subunit beta

Background

The rat Laminin Kit is a sandwich ELISA. The capture antibody is a polyclonal rat Laminin antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known Laminin concentration are added to these wells and allowed to complex with the bound Laminin antibody. A biotinylated rat Laminin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Laminin present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat Laminin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol LAMB2