DMC1 Antibody (1D12/4):
Western Blot
Procedure Guide for NB 100-181 Anti-hDMC-1
Western Blot
1. Proteins are separated on an SDS-PAGE gel.
2. Following the protein transfer, the membrane is blocked with 5% NFDM in TBS-T.
3. The membrane is then washed.
4. NB 100-181 [anti-hDMC] is diluted 1:1,000 in dilution buffer [5% NFDM in TBS-T] and incubated for 3 hours at room temperature, gently shaking.
5. The membrane is then washed.
6. Secondary antibody [HRP conjugated goat anti-mouse] is incubated for 45 minutes at room temperature, gently shaking.
7. Develop membrane.
NOTE: Mouse testis tissue extracts were used as a positive control for this antibody. MEF 161 and HeLa whole cell extracts were not shown to work in Western analysis.
