| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80962 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80963 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-237 | |||
| BSA Free | NB300-237SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04320SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-37235SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13177SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1598SS | |||
![Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0008.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At passage 5, cells were harvested and analyzed for pluripotent markers by flow cytometry. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A). Quadrant lines were drawn based on matched isotype controls.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Flow-Cytometry-NBA1-19349-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0007.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0009.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0010.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBP1-96751-img0003.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Brightfield Image of Dicer knock out Mouse embryonic stem cell line colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunohistochemistry-NBP1-96751-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Nanog antibody (NB100-58842) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBP1-96751-img0002.jpg)
![Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).](http://images.novusbio.com/fullsize/v6.5-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-Western-Blot-NBP1-97433-img0006.jpg "Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).")
![SDS-Page: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Ponceau Stain: Staining of v6.5 Mouse embryonic stem cells Lysate using NBP1-97433.](http://images.novusbio.com/fullsize/v6.5-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-SDS-Page-NBP1-97433-img0004.jpg)
![SDS-Page: Dicer knockout Mouse embryonic stem cells Whole Cell Lysate [NBP1-97435] - Staining of Dicer knockout Mouse embryonic stem cells Lysate using NBP1-97435.](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-SDS-Page-NBP1-97435-img0002.jpg "SDS-Page: Dicer knockout Mouse embryonic stem cells Whole Cell Lysate [NBP1-97435] - Staining of Dicer knockout Mouse embryonic stem cells Lysate using NBP1-97435.")
![Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - PSC characterisation. Human PSC-1 characterized for typical undifferentiated colony morphology in expression of pluripotency markers Nanog (Catalog # AF1997), OCT-3/4 (Catalog # AF1759), SSEA-3 (NB100-1832), SSEA-4 (Catalog # MAB1435) after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33899-6), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Immunocytochemistry-Immunofluorescence-NB100-1832-img0016.jpg "Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - PSC characterisation. Human PSC-1 characterized for typical undifferentiated colony morphology in expression of pluripotency markers Nanog (Catalog # AF1997), OCT-3/4 (Catalog # AF1759), SSEA-3 (NB100-1832), SSEA-4 (Catalog # MAB1435) after immunofluorescence staining. Corresponding nuclei stains with DAPI shown. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-33899-6), licensed under a CC-BY license.")
![Flow Cytometry: SSEA-3 Antibody (MC-631) [NB100-1832] - A surface stain was performed on Jurkat cells with SSEA-3 Antibody (MC-631) NB100-1832AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Flow-Cytometry-NB100-1832-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [NB100-1832] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody.](http://images.novusbio.com/fullsize/SSEA-3-Antibody-MC-631-Immunocytochemistry-Immunofluorescence-NB100-1832-img0012.jpg)
![Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Analysis using the FITC conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26645 (green histogram represents isotype control; red represents SSEA-4 antibody).](http://images.novusbio.com/fullsize/SSEA-4-Antibody-MC-813-70-Flow-Cell-Surface-NBP2-26644-img0007.jpg "Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Analysis using the FITC conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26645 (green histogram represents isotype control; red represents SSEA-4 antibody).")
![Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Cell surface analysis on 2102Ep cells using 0.1 ug/10^6 cells of NBP2-26644 (green histogram represents isotype control; red represents SSEA-4 antibody).](http://images.novusbio.com/fullsize/SSEA-4-Antibody-MC-813-70-Flow-Cytometry-NBP2-26644-img0002.jpg)
![Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Cell surface flow cytometric analysis of SSEA-4 PE on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26648 (green histogram represents isotype control; red represents SSEA-4 antibody).](http://images.novusbio.com/fullsize/SSEA-4-Antibody-MC-813-70-Flow-Cytometry-NBP2-26644-img0005.jpg)
![Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26647 (green histogram represents isotype control; red represents SSEA-4 antibody).](http://images.novusbio.com/fullsize/SSEA-4-Antibody-MC-813-70-Flow-Cell-Surface-NBP2-26644-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB100-58842-img0015.jpg "Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - The stem cell marker (Nanog) staining of iPS-BP cells. Nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Immunohistochemistry (IHC) for NANOG of paraffin-embedded sections of tail skin from CTR and TG mice treated as indicated in (B). Two magnifications are shown for each tissue (bars correspond to 50 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NB100-58842-img0011.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - Whole cell lysate (5, 15, and 50 ug) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB100-58842-img0009.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody [NB100-58842] - Nanog-inducible mouse model. Representative hematoxylin and eosin (H&E) staining and immunohistochemistry (IHC) for NANOG, Ki67 and LORICRIN of TPA-treated CTR and TG mice (bars correspond to 50 um). Statistical significance was determined by the two-tailed Student's t test: (*) p < 0.05; (**) p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep10205), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NB100-58842-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog staining in ES cells treatment with leukemia inhibitory factor (LIF). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0044024) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB100-58842-img0013.jpg)
![Immunohistochemistry: Nanog Antibody [NB100-58842] - Differentiation of wt and Suv4-20dn teratomas. Representative images of Nanog staining in wt and Suv4-20dn teratomas. Scale bar, 200 um. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0025680) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-NB100-58842-img0016.jpg)
![Immunoprecipitation: Nanog Antibody [NB100-58842] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from F9 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Nanog antibody used for IP at 6 ug per reaction. Nanog was also immunoprecipitated by rabbit anti-Nanog antibody. For blotting immunoprecipitated Nanog, this was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunoprecipitation-NB100-58842-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB100-58842] - Nanog antibody was tested in DGCR8 knockout Mouse embryonic stem cells (NBA1-19349) with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB100-58842-img0004.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - The forced expression of Tlk1 results in the aberrant downregulation of core pluripotency factors & attenuates self-renewal. (A) Immunoblot analysis of Oct4, Sox2, & Nanog levels in control mESCs (empty vector or doxycycline depletion) & Tlk1-overexpressing mESCs. The mESCs expressing an empty vector or the Tet-On-Tlk1 or Tet-On-Tlk1-D607A expression vector were cultured in the absence or presence of doxycycline (Dox; 100 ng/ml) for 24 hrs under undifferentiated self-renewal conditions. (B) Quantification of results from (A). The protein levels of the target genes were normalized to alpha -tubulin levels. The protein expression levels of each mESC line not treated with doxycycline were normalized to 1. The biological data are presented as mean (n = 6) ± SEM. *Р < 0.05, & **P < 0.01. (C) The morphology & AP staining of Tet-On-inducible Tlk1-expressing cell lines cultured in mock (Dox−) or doxycycline (Dox+) for 48 hrs. Scale bar, 500 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-31020241554197.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-310202415525256.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-310202415525252.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - Tlk1 is not required for mESC self-renewal or pluripotency. (A) The efficiency of Tlk1 knockdown (KD) in control (shLuc) & Tlk1-KD mESCs (shTlk1 #1 & #2) was confirmed by RT-qPCR analysis. Data are mean (n = 3) ± SEM. **Р < 0.01 & ***Р < 0.001. (B) The morphology of control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs was evaluated using phase-contrast microscopic images & AP staining. Scale bars represent 500 µm. (C & D) The mRNA expression of pluripotency-associated & development-associated genes were analyzed by RT-qPCR in control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs cultured under undifferentiated self-renewal conditions. All data were normalized to Gapdh & plotted relative to the expression level in control cells. Data are means (n = 3) ± SEM. *Р < 0.05, **Р < 0.01, & ***Р < 0.001. (E) The protein levels of pluripotency factors in control (shLuc) & Tlk1-KD (shTlk1 #1 & #2) mESCs was analyzed by immunoblotting using antibodies specific to Oct4, Sox2, & Nanog. (F) Quantification based on densitometry of Western blotting data from (E). All data were normalized to alpha -tubulin. Data are means (n = 3) ± SEM. *Р < 0.05, **Р < 0.01, & ***Р < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-310202415532014.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-310202415532036.jpg)
![Western Blot: Nanog Antibody [NB100-58842] - Tlk1-deficiency in mESCs causes a delay in the downregulation of core pluripotency factors upon differentiation. (A,C & E) Representative immunoblotting images showing Tlk1, Oct4, Sox2, & Nanog levels in Tlk1-KD cells upon differentiation. Differentiation was induced three different ways as previously described in Fig. 3. Alpha-tubulin was used as the loading control. (B,D & F) Quantification of the relative expression of the target proteins in panels (A,C, & E). The target proteins levels were normalized to that of alpha -tubulin. The protein expression levels of shLuc KD cells were normalized to 1. The biological data are presented as mean (n = 4) ± SEM for LIF- & EB & for RA (n = 3). *Р < 0.05, **P < 0.01, & ***P < 0.001. (G) Immunofluorescence analysis of Oct4, Nanog & Tlk1 in control (shLuc) & Tlk1-deficient mESCs. Scale bars represent 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29321513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-58842_rabbit-polyclonal-nanog-antibody-31020241683922.jpg)
![Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Western-Blot-NBP1-04320-img0001.jpg "Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunohistochemistry: Nanog Antibody (5A10) [NBP1-04320] - Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests and the stroma. Image collected and cropped by CiteAb from the following publication (journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0006.jpg)
![Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Flow-Cytometry-NBP1-04320-img0003.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodiumcitrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-Paraffin-NBP1-04320-img0004.jpg)
![Immunohistochemistry: Nanog Antibody (5A10) [NBP1-04320] - NANOG was seen in cells within the tumor nests [(F), brown, thick arrows] and the stroma [(F), brown, arrowheads], and the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. Image collected and cropped by CiteAb from the following publication (journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody (5A10) - BSA Free [NBP1-04320] - Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] & EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] & CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; & another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests & the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests & the stroma, & the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] & SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests & the stroma. Expression of both SOX2 [(F), red] & OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests & the stroma, & the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] & membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04320_mouse-nanog-mab-5a10-bsa-free-310202416165642.jpg)
![Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NB110-37235-img0008.jpg "Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.")
![Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunohistochemistry-Paraffin-NB110-37235-img0012.jpg)
![Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.](http://images.novusbio.com/fullsize/SOX2-Antibody-Flow-Cytometry-NB110-37235-img0015.jpg)
![Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NB110-37235-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SOX2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-37235-img0019.jpg)
![Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/SOX2-Antibody---BSA-Free-Flow-Cytometry-NB110-37235-img0020.jpg)
![Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NB110-37235-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37235-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37235-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SOX2-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-37235-img0018.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunohistochemistry-Paraffin-NBP2-13177-img0002.jpg "Immunohistochemistry-Paraffin: Nanog Antibody [NBP2-13177] - Staining of Nanog in mouse testis using DAB with hematoxylin counterstain.")
![Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Western-Blot-NB100-1598-img0013.jpg "Western Blot: CRIPTO Antibody [NB100-1598] - Total protein from human HeLa, NTERA-2 and spleen and embryonic mouse V6.5 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Cripto in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CRIPTO Antibody [NB100-1598] - CRIPTO antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1598-img0010.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on Jurkat with NB100-1598 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0012.jpg)
![Flow Cytometry: CRIPTO Antibody [NB100-1598] - An intracellular stain was performed on HeLa cells with NB100-1598PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Flow-Cytometry-NB100-1598-img0011.jpg)