Hu,
Mu,
Rt,
Po,
Bv,
Dr,
I,
Ma-Op,
PmSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Dr, I, Ma-Op, Pm
WB, Simple Western, ICC/IF, IHC, ChIP, KD
Applications: WB, Simple Western, ICC/IF, IHC, ChIP, KD
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Increased mitochondrial localization of CREB under hypoxiaA. Mitochondria were sub fractionated into outer membrane (OM), inter membrane space (IMS), inner membrane (IM) and mitochondrial matrix (MM). The purity of the fractions was analysed with marker proteins: TOM20 for OM, AIF for IMS, COX4 for IM, PDK4 for MM. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/10474/text/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Western-Blot-NB110-39115-img0023.jpg "Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Increased mitochondrial localization of CREB under hypoxiaA. Mitochondria were sub fractionated into outer membrane (OM), inter membrane space (IMS), inner membrane (IM) and mitochondrial matrix (MM). The purity of the fractions was analysed with marker proteins: TOM20 for OM, AIF for IMS, COX4 for IM, PDK4 for MM. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/article/10474/text/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-COX4 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-39115-img0021.jpg)
![Immunohistochemistry: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Immunohistochemistry-NB110-39115-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: COX4 Antibody - BSA Free [NB110-39115] - The mRNA and protein expression of cytochrome c oxidase 4 (COX4), toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), and nuclear factor (NF)-kB in lung tissues from spontaneous breathing group (CON group), normal tidal volume (NTV) group, high tidal volume (HTV) group, chloroquine (CQ) pretreatment upon HTV stimulation group (HTV + CQ). Levels of COX4, TLR9, MyD88, and NF-kB protein by Western blot. Fold expression for target genes was normalized to that measured for the beta-actin gene. Both of these experiments were in triplicate. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30018615/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/COX4 Antibody - BSA Free-Western Blot-NB110-39115-img0024.jpg)
![Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in the following cell lysates: 1. HeLa, 2. Ntera, 3. A431, 4. HepG2, 5. MCF7 and 6. 3T3.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Western-Blot-NB110-39115-img0012.jpg)
![Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Analysis of COX4 in porcine whole skeletal muscle lysate using anti-COX4 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Western-Blot-NB110-39115-img0016.jpg)
![Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Detection of COX4-1 isoform using COX4 antibody NB110-39115. Lane 1: HEK 293 with empty vector, Lane 2: HEK 293 with flag-tagged human COX4-1, Lane 3: HEK 293 with flag-tagged human COX4-2. Photo courtesy of Ryo Fukuda, G. Semenza lab. Johns Hopkins, SOM](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Western-Blot-NB110-39115-img0017.jpg)
![Western Blot: COX4 Antibody - BSA Free [NB110-39115] - Total protein from HeLa, 3T3 and PC12 was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-COX4 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Western-Blot-NB110-39115-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - COX4 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-39115-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: COX4 Antibody - BSA Free [NB110-39115] - Analysis of HeLa cells using COX4 antibody (NB110-39115, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-39115-img0014.jpg)
![Immunocytochemistry: COX4 Antibody - BSA Free [NB110-39115] - Analysis of methanol fixed drosophila embryo using 1:200 dilution of COX4 antibody. The signal was developed using AF488 conjugated Donkey anti-Rabbit IgG (H+L) secondary antibody and the sections were further counterstained for tubulin and DAPI. The antibody generated a specific staining of COX4 in mitochondria near mitotic spindles at early stage of embryo development. Image from verified customer review.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Immunocytochemistry-NB110-39115-img0019.jpg)
![Simple Western: COX4 Antibody - BSA Free [NB110-39115] - Image shows a specific band for COX4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/COX4-Antibody---BSA-Free-Simple-Western-NB110-39115-img0009.jpg)
![Western Blot: COX4 Antibody - BSA Free [NB110-39115] - The expression levels of microtubule protein light chain 3 (LC3B), PTEN inducing putative kinase 1 (PINK1), Parkin, mitofusin 1 (Mfn1), toll-like receptor (TLR) 9, cytochrome c oxidase 4 (COX4), myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON) or mechanical ventilation at high tidal volume (HTV) with saline, antimycin A (AmA) or cyclosporine A (CsA). (A) Levels of PINK1, Parkin, & Mfn1 mRNA. (B) Levels of LC3B, PINK1, Parkin, & Mfn1 protein by Western blot. (C) Relative expression of LC3B-II/LC3B-I & PINK1 protein. (D) Relative expression of Parkin & Mfn1 protein. (E) Levels of TLR9 & COX4 mRNA. (F) Levels of MyD88 & nuclear factor-kappa B (NF-kappa B) mRNA. (G) Levels of TLR9, COX4, MyD88, & NF-kappa B protein by Western blot. (H) Relative expression of TLR9 & COX4 protein. (I) Relative expression of MyD88 & NF-kappa B protein. Fold expression for target genes was normalized to that measured for the beta -actin gene. Both of these experiments were in triplicate. aP < 0.05 vs. CON group;bP < 0.05 vs. HTV group; & cP < 0.05 vs. AmA group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-39115_rabbit-polyclonal-cox4-antibody-3102024162494.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0012.jpg "Western Blot: COX4 Antibody (854) [NBP2-43540] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854)diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human colon cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min](http://images.novusbio.com/fullsize/COX4-Antibody-854-Immunohistochemistry-Paraffin-NBP2-43540-img0010.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug mouse heart lysate/extract 12 % SDS-PAGE COX4 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0003.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Analysis of A. 50 ug rat muscle lysate/extract 15 % SDS-PAGE COX4 antibody [854] dilution: 1:1000.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (854) [NBP2-43540] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [854]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0007.jpg)
![Western Blot: COX4 Antibody (854) [NBP2-43540] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (854) diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Western-Blot-NBP2-43540-img0011.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (854) [NBP2-43540] - Human ovarian cancer. COX4 stained by COX4 antibody [854] diluted at 1:200.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min.](http://images.novusbio.com/fullsize/COX4-Antibody-854-Immunohistochemistry-Paraffin-NBP2-43540-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody (854) [NBP2-43540] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-43540) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-43540_mouse-monoclonal-cox4-antibody-854-910202420134064.jpg)
![Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0002.jpg "Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (CL3497) [NBP2-78718] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Western-Blot-NBP2-78718-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0004.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0005.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3497) [NBP2-78718] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules and glomerulus cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3497-Immunohistochemistry-Paraffin-NBP2-78718-img0006.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0015.jpg "Western Blot: COX4 Antibody [NBP2-15975] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: COX4 stained by COX4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15975-img0014.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COX4 stained by COX4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunohistochemistry-Paraffin-NBP2-15975-img0016.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: rat muscle, 12% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/COX-IV-isoform-1-Antibody-Western-Blot-NBP2-15975-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody [NBP2-15975] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0006.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/COX4-Antibody-Western-Blot-NBP2-15975-img0013.jpg)
![Western Blot: COX4 Antibody [NBP2-15975] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (NBP2-15975) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-9102024204120.jpg)
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody [NBP2-15975] - COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: COX4 stained by COX4 antibody (NBP2-15975) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-91020242063363.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody [NBP2-15975] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15975_rabbit-polyclonal-cox4-antibody-1610202419414578.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human liver shows cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0005.jpg)
![Western Blot: COX4 Antibody (1327) [NBP2-59777] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2 and mouse cell line NIH-3T3.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Western-Blot-NBP2-59777-img0006.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0002.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0003.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59777] - Staining of human prostate shows cytoplasmic positivity in the epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-1327-Immunohistochemistry-Paraffin-NBP2-59777-img0004.jpg)
![Western Blot: COX4 Antibody (6310) [NBP2-43629] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [6310].](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Western-Blot-NBP2-43629-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: COX4 Antibody (6310) [NBP2-43629] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [6310].")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: COX4 Antibody (6310) [NBP2-43629] - Analysis of HeLa cells were fixed in -20? 100% methanol for 5 min. Green: COX4I1 protein stained by COX4I1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Immunocytochemistry-Immunofluorescence-NBP2-43629-img0001.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (6310) [NBP2-43629] - Analysis of U87 xenograft. COX4 antibody [6310] dilution: 1:200.](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Immunohistochemistry-Paraffin-NBP2-43629-img0005.jpg)
![Western Blot: COX4 Antibody (6310) [NBP2-43629] - Analysis of A. 50 ug rat muscle lysate/extract 12 % SDS-PAGE COX4 antibody [6310] dilution: 1:1000](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Western-Blot-NBP2-43629-img0004.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (6310) [NBP2-43629] - Analysis of hepatoma. COX4 antibody [6310] dilution: 1:200.](http://images.novusbio.com/fullsize/COX4-Antibody-6310-Immunohistochemistry-Paraffin-NBP2-43629-img0002.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (6310) [NBP2-43629] - COX4 antibody [GT6310] detects COX4 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.COX4 stained by COX4 antibody [GT6310] (NBP2-43629) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43629_mouse-monoclonal-cox4-antibody-6310-9102024201576.jpg)
![Western Blot: COX4 Antibody (6310) [NBP2-43629] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (NBP2-43629) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43629_mouse-monoclonal-cox4-antibody-6310-91020242011468.jpg)
![Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Western-Blot-NBP2-61620-img0006.jpg "Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLaLane 3: Human cell line HEK 293Lane 4: Human cell line A-431Lane 5: Human cell line HepG2")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620] - Staining of MCF7 cells using the Anti-COX4I1 monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunocytochemistry-Immunofluorescence-NBP2-61620-img0007.jpg)
![Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human skin shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-Paraffin-NBP2-61620-img0005.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0001.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human placenta shows strong cytoplasmic immunoreactivity in trophoblasts.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0002.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0003.jpg)
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0004.jpg)
![Western Blot: COX4 Antibody (6B3) [NBP1-51650] - Analysis using COX4I1 mouse mAb against HEK293 (1), A549 (2) and PC12 (3) cell lysate.](http://images.novusbio.com/fullsize/COX4-Antibody-6B3-Western-Blot-NBP1-51650-img0011.jpg "Western Blot: COX4 Antibody (6B3) [NBP1-51650] - Analysis using COX4I1 mouse mAb against HEK293 (1), A549 (2) and PC12 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: COX4 Antibody (6B3) [NBP1-51650] - Analysis of PANC-1 cells using COX4I1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/COX4-Antibody-6B3-Immunocytochemistry-Immunofluorescence-NBP1-51650-img0010.jpg)
![Flow Cytometry: COX4 Antibody (6B3) [NBP1-51650] - Analysis of K562 cells using COX4I1 mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/COX4-Antibody-6B3-Flow-Cytometry-NBP1-51650-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: COX4 Antibody [NBP1-85498] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & mitochondria.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85498-img0006.jpg "Immunocytochemistry/Immunofluorescence: COX4 Antibody [NBP1-85498] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & mitochondria.")
![Immunohistochemistry-Paraffin: COX4 Antibody [NBP1-85498] - Staining of human kidney shows moderate granular positivity in renal tubules.](http://images.novusbio.com/fullsize/COX4-Antibody-Immunohistochemistry-Paraffin-NBP1-85498-img0005.jpg)
![Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Western-Blot-NBP3-15416-img0002.jpg "Western Blot: COX4 Antibody (9X9I0) [NBP3-15416] - Western blot analysis of extracts of various cell lines, using COX4 Rabbit mAb (NBP3-15416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: COX4 Antibody (9X9I0) [NBP3-15416] - Immunohistochemistry of paraffin-embedded human colon carcinoma using COX4 Rabbit mAb (NBP3-15416) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/COX4-Antibody-9X9I0-Immunohistochemistry-Paraffin-NBP3-15416-img0001.jpg)
![Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/COX-IV-[p-Ser58]-Antibody-Western-Blot-NBP2-29532-img0001.jpg "Western Blot: COX IV [p Ser58] Antibody [NBP2-29532] - Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17k COXIV phosphorylated at Ser58 (Control). Immunolabeling is blocked by the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")