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![Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0008.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - SSEA1 antibody (NB100-1831) was tested in with DyLight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![<b>Genetic Strategies Validation. </b>Flow Cytometry: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - DCGR8 mouse embryonic stem cells were cultured for 5 passages in StemXVivo Mouse Pluripotent Stem Cell Media (R&D Systems, Catalog # CCM025). At passage 5, cells were harvested and analyzed for pluripotent markers by flow cytometry. Expression of SSEA-1 and SSEA-4 were detected using PE-conjugated Mouse Anti-SSEA-1 Monoclonal Antibody (R&D Systems, Catalog # FAB2155P) and APC-conjugated Mouse Anti-SSEA-4 Monoclonal Antibody (R&D Systems, Catalog # FAB1435A). Quadrant lines were drawn based on matched isotype controls.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Flow-Cytometry-NBA1-19349-img0012.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Nanog antibody (NB100-58842) was tested in with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBA1-19349-img0007.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0009.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 knockout Mouse embryonic stem cell [NBA1-19349] colonies growing on a Mitomycin C MEF cell feeder layer.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0010.jpg)
![In vitro assay: DGCR8 knockout Mouse embryonic stem cells [NBA1-19349] - Brightfield Image of DGCR8 -/- ES cell colonies growing on a gamma-irradiated MEF cell feeder layer.](http://images.novusbio.com/fullsize/DGCR8-knockout-Mouse-embryonic-stem-cells-In-vitro-assay-NBA1-19349-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBP1-96751-img0003.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Brightfield Image of Dicer knock out Mouse embryonic stem cell line colonies growing directly on a gelatinized tissue culture flask.](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunohistochemistry-NBP1-96751-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Nanog antibody (NB100-58842) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Dicer-knockout-Mouse-embryonic-stem-cells-Immunocytochemistry-Immunofluorescence-NBP1-96751-img0002.jpg)