Hu,
Mu,
Rt,
Po,
Am,
Ca,
Ch,
Fe,
Ha,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, Sh
WB, Flow, ICC/IF, IHC, IP
Applications: WB, Flow, ICC/IF, IHC, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunocytochemistry-Immunofluorescence-NB300-569-img0005.jpg "Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.")
![Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunohistochemistry-Paraffin-NB300-569-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image. Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunocytochemistry-Immunofluorescence-NB300-569-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.](http://images.novusbio.com/fullsize/PMCA4-Antibody-JA9-Immunocytochemistry-Immunofluorescence-NB300-569-img0004.jpg)
![Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Western-Blot-NB300-578-img0015.jpg "Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).")
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0014.jpg)
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunocytochemistry-Immunofluorescence-NB300-578-img0005.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0012.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Immunohistochemistry-Paraffin-NB300-578-img0013.jpg)
![Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).](http://images.novusbio.com/fullsize/PMCA1-Antibody-5F10-Flow-Cytometry-NB300-578-img0010.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Endogenous expression of PMCA4b in WT and renalase KO mice, western immunoblot using an anti-renalase monoclonal antibody; 10 ug protein loaded in each lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0005.jpg "Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Endogenous expression of PMCA4b in WT and renalase KO mice, western immunoblot using an anti-renalase monoclonal antibody; 10 ug protein loaded in each lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein. Co-immunolocalization of PMCA4b and renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 um; arrow = plasma membrane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Immunocytochemistry-Immunofluorescence-H00000493-M07-img0006.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein. Co-Immunoprecipitation of PMCA4b and renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0002.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. siRNA mediated inhibition of PMCA4b expression downregulates RP-220 mediated MAPK signaling; left panel: RP-220 mediated ERK and p38 activation in HK-2 cells transfected with non-targeting siRNA, p = phosphorylated, representative immunoblot; middle panel: Inhibition of RP-220 mediated ERK and p38 activation in HK-2 cells transfected with PMCA4b siRNA, representative blot; right panel: quantification of phosphorylated ERK (p-ERK), signals normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) loading control; n = 3, * = P<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0003.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - PMCA4b inhibition abrogates renalase peptide mediated MAPK signaling and cytoprotection. Lack of effect of siRNA mediated inhibition of PMCA4b expression on epidermal growth factor (EGF)- mediated MAPK signaling; left panel: EGF (100 ng/ml) mediated ERK, p38 activation and c-Jun N-Terminal Kinase (JNK) in HK-2 cells transfected with non-targeting siRNA, p = phosphorylated, representative blot; right panel: EGF-mediated ERK, p38 and JNK activation in HK-2 cells unaffected by transfection with PMCA4b siRNA and downregulation of PMCA4b expression; representative blot (n = 3). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0122932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Western-Blot-H00000493-M07-img0004.jpg)
![Sandwich ELISA: PMCA4 Antibody (2G8) [H00000493-M07] - Detection limit for recombinant GST tagged ATP2B4 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PMCA4-Antibody-2G8-Sandwich-ELISA-H00000493-M07-img0001.jpg)
![Western Blot: PMCA4 Antibody (2G8) [H00000493-M07] - Identification of plasma membrane calcium ATPase isoform PMCA4b as a renalase binding protein.A, HK-2 cells incubated with either labeled RP-Scr220 or RP-220, biotin-labeled proteins purified using streptavidin column, separated by SDS-PAGE & visualized by western blot using streptavidin-HRP; * = regions evaluated by mass spectrometry in samples labeled with either RP-Scr220 or RP-220; # = RP-220 band containing the plasma membrane calcium ATPase isoform PMCA4b. B, Endogenous expression of PMCA4b in HK-2 cells, western immunoblot using isoform specific monoclonal; CCL-119: human leukemic cell line; thyroid tumor = human thyroid tumor cell line (ATCC, CRL-1803) 10 μg protein loaded in each lane. C, co-immunolocalization of PMCA4b & renalase in HK-2 cells, images acquired using a Zeiss laser scanning confocal microscope, scale bar = 9 μm; arrow = plasma membrane. D, Co-Immunoprecipitation of PMCA4b & renalase from HK-2 cell lysates; renalase-Ab-beads = renalase antibody coated beads; PMCA4b-Ab-beads = PMCA4b antibody coated beads. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25906147), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000493-m07_mouse-monoclonal-pmca4-antibody-2g8-310202415482242.jpg)
![Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP1-87259 antibody. Corresponding PMCA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunohistochemistry-Paraffin-NBP1-87259-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Analysis in human cerebral cortex and skeletal muscle tissues using NBP1-87259 antibody. Corresponding PMCA3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PMCA3 Antibody [NBP1-87259] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87259-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human cerebellum shows strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunohistochemistry-Paraffin-NBP1-87259-img0012.jpg)
![Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunohistochemistry-Paraffin-NBP1-87259-img0013.jpg)
![Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunohistochemistry-Paraffin-NBP1-87259-img0015.jpg)
![Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunohistochemistry-Paraffin-NBP1-87259-img0014.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87261-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PMCA1 Antibody [NBP1-87261] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 10. Lane 2: Human Cerebral Cortex tissue.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP1-87261-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody [NBP1-87261] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus & plasma membrane.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87261-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87261-img0008.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87261] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87261-img0009.jpg)
![Western Blot: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP3-12964-img0001.jpg "Western Blot: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein by western blot analysis. HeLa whole cell extracts and membrane extracts(the extracts were unboiled )(30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PMCA1 antibody (NBP3-12964) at a dilution of 1:1000.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: PMCA1 protein stained by PMCA1 antibody (NBP3-12964) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP3-12964-img0004.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP3-12964-img0002.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP3-12964] - PMCA1 antibody detects PMCA1 protein at cell membrane and cytoplasm in rat prostate by immunohistochemical analysis. Sample: Paraffin-embedded rat prostate. PMCA1 antibody (NBP3-12964) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP3-12964-img0003.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87260-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining in human cerebral cortex and kidney tissues using anti-ATP2B1 antibody. Corresponding ATP2B1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PMCA1 Antibody [NBP1-87260] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Western-Blot-NBP1-87260-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87260-img0004.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NBP1-87260] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NBP1-87260-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA4 Antibody [NBP2-31984] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31984-img0005.jpg "Immunocytochemistry/Immunofluorescence: PMCA4 Antibody [NBP2-31984] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMCA4 Antibody [NBP2-31984] - Staining in human fallopian tube and pancreas tissues using anti-ATP2B4 antibody. Corresponding ATP2B4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunohistochemistry-Paraffin-NBP2-31984-img0008.jpg)
![Immunohistochemistry-Paraffin: PMCA4 Antibody [NBP2-31984] - Staining of human fallopian tube shows high expression.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunohistochemistry-Paraffin-NBP2-31984-img0006.jpg)
![Immunohistochemistry-Paraffin: PMCA4 Antibody [NBP2-31984] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PMCA4-Antibody-Immunohistochemistry-Paraffin-NBP2-31984-img0007.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NB120-3528] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NB120-3528-img0004.jpg "Immunohistochemistry-Paraffin: PMCA1 Antibody [NB120-3528] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.")
![Immunohistochemistry-Paraffin: PMCA1 Antibody [NB120-3528] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA1 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/PMCA1-Antibody-Immunohistochemistry-Paraffin-NB120-3528-img0003.jpg)
![Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.](http://images.novusbio.com/fullsize/PMCA2-Antibody-Immunohistochemistry-Paraffin-NB120-3529-img0004.jpg "Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.")
![Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Cancer biopsies of deparaffinized Human cervical carcinoma tissue.](http://images.novusbio.com/fullsize/PMCA2-Antibody-Immunohistochemistry-Paraffin-NB120-3529-img0005.jpg)
![Western Blot: PMCA1 Antibody (JE34-05) [NBP3-32830] - Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/1,000 dilution. Lane 1: A549 cell lysate (no heat) Lane 2: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 135 kDa Observed band size: 135-250 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32830_rabbit-pmca1-mab-je34-05-288202412442991.jpg "Western Blot: PMCA1 Antibody (JE34-05) [NBP3-32830] - Western blot analysis of PMCA1 on different lysates with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/1,000 dilution. Lane 1: A549 cell lysate (no heat) Lane 2: A549 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 135 kDa Observed band size: 135-250 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32830) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMCA1 Antibody (JE34-05) [NBP3-32830] - Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PMCA1 antibody (NBP3-32830) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32830) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32830_rabbit-pmca1-mab-je34-05-29820241435250.jpg)
![Western Blot: PMCA3 Antibody [NBP2-19871] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Western-Blot-NBP2-19871-img0001.jpg "Western Blot: PMCA3 Antibody [NBP2-19871] - Sample (30 ug of whole cell lysate) A: U87-MG 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP2-19871] - U87 xenograft, using ATP2B3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PMCA3-Antibody-Immunohistochemistry-Paraffin-NBP2-19871-img0002.jpg)