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13 Results for "lap tgf beta 1"
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AF-246-NA

LAP (TGF-beta 1) Antibody [Unconjugated]

(3 Reviews)
(34 Publications)
100 μg
AF-246-NA
Western blot shows lysates of Saos-2 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LAP (TGF-beta 1) at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, B/N, ICC/IF
Applications: WB, Simple Western, B/N, ICC/IF
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Biotin BAF246
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF-246-SP
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NBP2-22114

LAP (TGF-beta 1) Antibody (7F6) - BSA Free

(3 Reviews)
(17 Publications)
0.1 ml
NBP2-22114
Western Blot: LAP (TGF-beta 1) Antibody (7F6) [NBP2-22114] - Western blot analysis using TGF beta 1 mAb against human LAP (TGF-beta 1) recombinant protein. (Expected MW is 41 kDa)
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ELISA, Flow, IHC
Applications: WB, ELISA, Flow, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-22114AF350
Alexa Fluor 405 NBP2-22114AF405
Alexa Fluor 488 NBP2-22114AF488
Alexa Fluor 532 NBP2-22114AF532
Alexa Fluor 594 NBP2-22114AF594
Alexa Fluor 647 NBP2-22114AF647
Alexa Fluor 700 NBP2-22114AF700
Alexa Fluor 750 NBP2-22114AF750
Biotin NBP2-22114B
CoraFluor 1 NBP2-22114CL1
DyLight 350 NBP2-22114UV
DyLight 405 NBP2-22114V
DyLight 488 NBP2-22114G
DyLight 550 NBP2-22114R
DyLight 594 NBP2-22114DL594
DyLight 650 NBP2-22114C
DyLight 680 NBP2-22114FR
DyLight 755 NBP2-22114IR
FITC NBP2-22114F
HRP NBP2-22114H
Janelia Fluor 549 NBP2-22114JF549
Janelia Fluor 646 NBP2-22114JF646
mFluor Violet 450 SE NBP2-22114MFV450
mFluor Violet 500 SE NBP2-22114MFV500
mFluor Violet 610 SE NBP2-22114MFV610
Available Formulations
Formulation Catalog # Availability Price  
BSA Free NBP2-22114SS
Azide and BSA Free NBP2-80827
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AB-246-NA

LAP (TGF-beta 1) Antibody [Unconjugated]

(1 Review)
(11 Publications)
1 mg
AB-246-NA
TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, B/N, ICC/IF
Applications: WB, IHC, B/N, ICC/IF
Goat Polyclonal
Host: Goat Polyclonal
Unconjugated  
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MAB246

LAP (TGF-beta 1) Antibody (27235) [Unconjugated]

(13 Publications)
100 μg
MAB246
LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Hu
Species Glossary
Reactivity: Hu
B/N, ICC/IF
Applications: B/N, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB246-SP
Carrier Free MAB246-500
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MAB2463

LAP (TGF-beta 1) Antibody (27232) [Unconjugated]

(10 Publications)
500 μg
Hu
Species Glossary
Reactivity: Hu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2463U-100UG
Alexa Fluor 405 FAB2463V-100UG
Alexa Fluor 488 FAB2463G
Alexa Fluor 532 FAB2463X
Alexa Fluor 594 FAB2463T-100UG
Alexa Fluor 647 FAB2463R-100UG
Alexa Fluor 750 FAB2463S-100UG
Allophycocyanin FAB2463A
Allophycocyanin/Cy7 FAB2463APCCY7
Biotin FAB2463B
DyLight 350 FAB2463D
DyLight 405 FAB2463E
DyLight 488 FAB2463K
DyLight 550 FAB2463L
DyLight 594 FAB2463M
DyLight 650 FAB2463W
DyLight 680 FAB2463Y
DyLight 755 FAB2463Z
FITC FAB2463F
HRP FAB2463H
Janelia Fluor 525 FAB2463JF525
Janelia Fluor 549 FAB2463I
Janelia Fluor 646 FAB2463J
Janelia Fluor 669 FAB2463JF669
PE/Atto594 FAB2463PEATT594
PE/Cy5.5 FAB2463PECY55
PE/Cy7 FAB2463PECY7
Phycoerythrin FAB2463P
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free FAB2463A
Carrier Free FAB2463G
Carrier Free FAB2463P
Carrier Free MAB2463-SP
Carrier Free FAB2463V-100UG
Carrier Free FAB2463U-100UG
Carrier Free FAB2463T-100UG
Carrier Free FAB2463S-100UG
Carrier Free FAB2463R-100UG
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MAB77541

LAP (TGF-beta 1) Antibody (1015022) [Unconjugated]

100 μg
MAB77541
PC-3 human prostate cancer cell line was stained with Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Hu
Species Glossary
Reactivity: Hu
ELISA, CyTOF-ready, Flow, ICC/IF
Applications: ELISA, CyTOF-ready, Flow, ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC77541U-100UG
Alexa Fluor 405 IC77541V-100UG
Alexa Fluor 488 IC77541G-100UG
Alexa Fluor 532 FAB77541X
Alexa Fluor 594 IC77541T-100UG
Alexa Fluor 647 IC77541R-100UG
Alexa Fluor 700 IC77541N-100UG
Alexa Fluor 750 IC77541S-100UG
Allophycocyanin FAB77541A
Biotin FAB77541B
DyLight 350 FAB77541D
DyLight 405 FAB77541E
DyLight 488 FAB77541K
DyLight 550 FAB77541L
DyLight 594 FAB77541M
DyLight 650 FAB77541W
DyLight 680 FAB77541Y
DyLight 755 FAB77541Z
FITC FAB77541F
HRP FAB77541H
Janelia Fluor 549 FAB77541I
Janelia Fluor 646 FAB77541J
PE FAB77541P
PerCP FAB77541C
mFluor Violet 450 SE FAB77541MFV450
mFluor Violet 500 SE FAB77541MFV500
mFluor Violet 610 SE FAB77541MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB77541-SP
Carrier Free IC77541V-100UG
Carrier Free IC77541U-100UG
Carrier Free IC77541T-100UG
Carrier Free IC77541S-100UG
Carrier Free IC77541R-100UG
Carrier Free IC77541N-100UG
Carrier Free IC77541G-100UG
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MAB2461

LAP (TGF-beta 1) Antibody (9005)

(2 Publications)
100 μg
Hu
Species Glossary
Reactivity: Hu
ELISA(Cap)
Applications: ELISA(Cap)
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2461-SP
Carrier Free MAB2461-500
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MAB7754

LAP (TGF-beta 1) Antibody (1015016) [Unconjugated]

100 μg
MAB7754
Recombinant Human LAP (TGF‑ beta 1) protein was serially diluted 2-fold and captured by Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB77541) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human LAP (TGF‑ beta 1) Monoclonal Antibody (Catalog # MAB7754) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB7754-SP
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NBP3-21360

LAP (TGF-beta 1) Antibody

100 ul
NBP3-21360
Staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.
Hu
Species Glossary
Reactivity: Hu
ICC/IF
Applications: ICC/IF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
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BAF246

LAP (TGF-beta 1) Antibody [Biotin]

(17 Publications)
50 μg
BAF246
Disulfide-linked GARP/TGF-beta 1 complexes are released in the supernatant of T cells, but not 293 cells.A. Cells described in Figure 2 were lysed and immunoprecipitated (IP) with anti-GARP or anti-LAP antibodies. IP products were submitted to SDS-PAGE under non-reducing or reducing conditions, followed by WB with anti-LAP antibodies (top and middle panels), or anti-GARP antibodies (bottom panels). Pro-TGF-beta 1 and LAP homodimers in the top panels are not clearly resolved, but can be distinguished better with longer migrations or higher concentrations of polyacrylamide. The +/- 85-90 kDa bands that appear in the middle panel correspond to non-specific bands, or to incompletely reduced pro-TGF-beta 1. B. Cells (2x106/ml for murine and human T cells, 2.5x105/ml for transfected 293 cells) were incubated in serum free medium during 24 hours. Different cell concentrations were used to adjust for the different amounts of secreted TGF-beta 1 (see Figure 2). Human Th A2 and Jurkat cells were stimulated with anti-CD3/CD28 antibodies to increase secretion. Supernatants (0.5-10 µl) were analyzed by WB under non-reducing conditions with anti-GARP and anti-LAP antibodies. * Band that also appears when the secondary anti-IgG2b-HRP antibody is used alone (without anti-GARP antibody), due to cross reactivity against the anti-CD3/CD28 antibodies used for T cell stimulation. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0076186), licensed under a CC-BY license. Not internally tested by R&D Systems.
Hu
Species Glossary
Reactivity: Hu
WB
Applications: WB
Goat Polyclonal
Host: Goat Polyclonal
Biotin  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF-246-NA
Carrier Free AF-246-SP
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MAB7666

LAP (TGF-beta 1) Antibody (860206) [Unconjugated]

(12 Publications)
100 μg
MAB7666
Mouse splenocytes treated with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 μg/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), and 20 μg/mL Recombinant Mouse IL‑2 (Catalog # 402-IL) for 24 hours to induce T regulatory cells (iTregs) were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse LAP (TGF‑ beta 1) Monoclonal Antibody (Catalog # MAB7666) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Mu
Species Glossary
Reactivity: Mu
Flow, CyTOF-ready
Applications: Flow, CyTOF-ready
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB7666U-100UG
Alexa Fluor 405 FAB7666V-100UG
Alexa Fluor 488 FAB7666G-100UG
Alexa Fluor 532 FAB7666X
Alexa Fluor 594 FAB7666T-100UG
Alexa Fluor 647 FAB7666R-100UG
Alexa Fluor 700 FAB7666N-100UG
Alexa Fluor 750 FAB7666S-100UG
Allophycocyanin FAB7666A
Allophycocyanin/Cy7 FAB7666APCCY7
Biotin FAB7666B
CoraFluor 1 FAB7666CL1
DyLight 350 FAB7666D
DyLight 405 FAB7666E
DyLight 488 FAB7666K
DyLight 550 FAB7666L
DyLight 594 FAB7666M
DyLight 650 FAB7666W
DyLight 680 FAB7666Y
DyLight 755 FAB7666Z
FITC FAB7666F
HRP FAB7666H
Janelia Fluor 525 FAB7666JF525
Janelia Fluor 549 FAB7666I
Janelia Fluor 646 FAB7666J
Janelia Fluor 669 FAB7666JF669
PE FAB7666P
PE/Atto594 FAB7666PEATT594
PE/Cy5.5 FAB7666PECY55
PE/Cy7 FAB7666PECY7
PerCP FAB7666C
mFluor Violet 450 SE FAB7666MFV450
mFluor Violet 500 SE FAB7666MFV500
mFluor Violet 610 SE FAB7666MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB7666-SP
Carrier Free FAB7666V-100UG
Carrier Free FAB7666U-100UG
Carrier Free FAB7666T-100UG
Carrier Free FAB7666S-100UG
Carrier Free FAB7666R-100UG
Carrier Free FAB7666N-100UG
Carrier Free FAB7666G-100UG
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BAM2462

LAP (TGF-beta 1) Antibody (27240) [Biotin]

(1 Review)
(5 Publications)
50 μg
Hu
Species Glossary
Reactivity: Hu
ELISA(Det)
Applications: ELISA(Det)
Mouse Monoclonal
Host: Mouse Monoclonal
Biotin  
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