| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1537-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF15371-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1665-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52513-0.025mg | |||
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0010.jpg "Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.")
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-NB600-607-img0011.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![TOR/mTOR [p Ser2448] Antibody [NB600-607]](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![ELISA: TOR/mTOR [p Ser2448] Antibody [NB600-607] - ELISA Results of TOR/TOR/mTOR [p Ser2448] antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-ELISA-NB600-607-img0012.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mTOR phosphorylation during microglial activation(A) Whole-cell lysates were prepared from microglial cells incubated for 2 h as indicated. Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel. The same blots were subsequently probed for beta -actin, lower gel. (B) Quantitation of densitometry where p-mTOR values are reflected relative to those for beta -actin. Data are expressed as means±S.E.M. of n=1 replicate for each group, each assayed in triplicates. Representative of two different experiments. Data were analyzed by one-way ANOVA followed by the Bonferroni's post hoc test. ***P<0.001 versus Control, °P<0.05 versus C-CM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24689533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-607_rabbit-polyclonal-tor-mtor-p-ser2448-antibody-31020241535647.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mammalian target of rapamycin (mTOR) phosphorylation during microglial activation. Whole-cell lysates were prepared from microglial cells activated by control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFN gamma (LI-CM) (B). Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel & were subsequently probed for beta -actin, lower gel. Quantitation of densitometry where p-mTOR values are reflected relative to those for beta -actin. Data are expressed as means ± standard error of the mean of n = 1 replicate for each group, each assayed in triplicate: representative of two different experiments. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test. ***P <0.001 versus control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25051975), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-607_rabbit-polyclonal-tor-mtor-p-ser2448-antibody-310202416171492.jpg)
![Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - TOR/mTOR (phospho Ser2448) antibody detects TOR/mTOR (phospho Ser2448) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right). Sample: Paraffin-embedded human lung cancer. TOR/mTOR (phospho Ser2448) stained by TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NBP3-13144-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TOR/mTOR (phospho Ser2448) antibody (NBP3-13144) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NBP3-13144-img0002.jpg)
![ICC/IF: TOR/mTOR [p Ser2448] Antibody [NBP3-13144] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: mTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp3-13144_rabbit-tor-mtor-p-ser-2448-pab-immunocytochemistry-immunofluorescence-126202482926..jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0003.jpg "Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against human mTOR (AA: 2311-2529) recombinant protein. (Expected MW is 50.8 kDa)")
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of endometrial cancer tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0002.jpg)
![Western Blot: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis using mTOR mAb against HEK293 (1) and mTOR (AA: 2311-2529)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Western-Blot-NBP2-52513-img0004.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Analysis of brain tissues using mTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-Immunohistochemistry-NBP2-52513-img0001.jpg)
![ELISA: TOR/mTOR Antibody (8H5A5) [NBP2-52513] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-8H5A5-ELISA-NBP2-52513-img0005.jpg)
![Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [6611].](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-6611-Western-Blot-NBP2-43658-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [6611].")
![<b>Genetic Strategies Validation. </b>Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A Jurkat whole cell lysate/extract B Raji C NCI-H929 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:1000.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-6611-Western-Blot-NBP2-43658-img0001.jpg)
![Western Blot: TOR/mTOR Antibody (6611) [NBP2-43658] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE mTOR antibody [6611] dilution: 1:500](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-6611-Western-Blot-NBP2-43658-img0002.jpg)
![TOR/mTOR Antibody (6611) [NBP2-43658] -HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mTOR stained by mTOR antibody [6611] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/nbp2-43658_mouse-monoclonal-tor-mtor-antibody-6611-immunocytochemistry-immunofluorescence-15202313627..jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-2622024152248.jpg "Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Image of TOR/mTOR [p Ser2448] Antibody (1G6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Positive Western Blot detected in Hela whole cell lysate, A549 whole cell lysate. All lanes: TOR/mTOR [p Ser2448] Antibody at 0.825ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241140938.jpg)
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (1G6) [NBP3-26406] - Staining of Hela cells with TOR/mTOR [p Ser2448] Antibody (1G6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26406_rabbit-tor-mtor-p-ser-2448-mab-1g6-28220241312233.jpg)
![Western Blot: mTOR Antibody (F11) [NBP2-00217] - Reduced cell lysates prepared from Jurkat cell line with 5 ug/mL of Anti-Human/Mouse mTOR Purified. Bands were visualized using Anti-Mouse IgG HRP.](http://images.novusbio.com/fullsize/mTOR-Antibody-F11-Western-Blot-NBP2-00217-img0001.jpg "Western Blot: mTOR Antibody (F11) [NBP2-00217] - Reduced cell lysates prepared from Jurkat cell line with 5 ug/mL of Anti-Human/Mouse mTOR Purified. Bands were visualized using Anti-Mouse IgG HRP.")
![Western Blot: TOR/mTOR Antibody (RM274) [NBP2-61590] - Western Blot of HeLa cells lysates using NBP2-61590.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-RM274-Western-Blot-NBP2-61590-img0005.jpg "Western Blot: TOR/mTOR Antibody (RM274) [NBP2-61590] - Western Blot of HeLa cells lysates using NBP2-61590.")
![Immunohistochemistry-Paraffin: TOR/mTOR Antibody (RM274) [NBP2-61590] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61590.](http://images.novusbio.com/fullsize/TOR-mTOR-Antibody-RM274-Immunohistochemistry-Paraffin-NBP2-61590-img0002.jpg)
![Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Positive Western Blot detected in A549 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF). All lanes: TOR/mTOR [p Ser2481] Antibody at 1.33ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa](http://images.novusbio.com/fullsize/nbp3-26405_rabbit-tor-mtor-p-ser-2481-mab-3h11-28220241141360.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Positive Western Blot detected in A549 whole cell lysate, 293 whole cell lysate (treated with Calyculin A or EGF). All lanes: TOR/mTOR [p Ser2481] Antibody at 1.33ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 289 KDa Observed band size: 289 KDa")
![Immunocytochemistry/Immunofluorescence: TOR/mTOR [p Ser2481] Antibody (3H11) [NBP3-26405] - Staining of Hela cells with TOR/mTOR [p Ser2481] Antibody (3H11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26405_rabbit-tor-mtor-p-ser-2481-mab-3h11-282202412475139.jpg)