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![Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SUCNR1/GPR91 Antibody NBP1-00861 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00861-img0019.jpg "Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SUCNR1/GPR91 Antibody NBP1-00861 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - D) SUCNR1 protein expression relative to B-tubulin in vitro. Data are shown as mean (+/- SEM) of n >= 3 independent replicates per condition. (H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S and expressed as mean fold change (+/- SEM) versus NSCs from n >= 3 independent replicates per condition. (I) Representative blot of SUNCR1 basal protein expression in human cells. hBJFs, human BJ fibroblasts. *p <= 0.05 versus 0'. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29478844) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0020.jpg)
![Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NBP1-00861] - Staining of GPR91 in mouse Vas Deferens where strong membrane staining is observed.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunohistochemistry-Paraffin-NBP1-00861-img0015.jpg)
![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of human kidney tissue (A), mouse kidney tissue (B), rat kidney tissue (C), and hek293 cells (D) using GPR91 antibody at 2ug/ml.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0016.jpg)
![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat kidney tissue lysate.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0017.jpg)
![Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of GPR91 (A135) antibody in extracts from HUVEC/MCF-7 cells.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP1-00861-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GPR91 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00861-img0014.jpg)
![Western Blot: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861] - Succinate Signals via SUCNR1 in Mouse & Human NSCs(A–C) Representative confocal microscopy images of meningeal perivascular areas with transplanted fGFP+ iNSCs (A) & NSCs (B) expressing SUCNR1 in the brain of a mouse with EAE. The image in (C) shows transplanted SUCNR1+ iNSCs in close vicinity to SUCNR1+/F4/80+ MPs. Nuclei are stained with DAPI.(D) SUCNR1 protein expression relative to beta -tubulin in vitro. Data are shown as mean (±SEM) of n ≥ 3 independent replicates per condition.(E) Experimental setup for succinate treatment of iNSCs/NSCs in vitro.(F) Intracellular Ca2+ response after treatment with 500 μM succinate (live staining with Fluo-4AM). Representative images (baseline & during stimulation) are pseudocolored with red/blue according to high/low fluorescence intensity. Data are mean changes in fluorescence intensity as delta F/F0 (±SEM) from n ≥ 3 experiments.(G) Phospho-p38 MAPK (P-p38) & total p38 MAPK (p38) protein expression after succinate treatment. Data are P-p38/p38 expression relative to beta -tubulin & expressed as mean fold change (±SEM) versus untreated cells over n ≥ 3 independent experiments per condition.(H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S & expressed as mean fold change (±SEM) versus NSCs from n ≥ 3 independent replicates per condition.(I) Representative blot of SUNCR1 basal protein expression in human cells.(J) P-p38 & p38 protein expression after stimulation with succinate ± pre-treatment with the irreversible inhibitor of the human SUCNR1 4c.The scale bars represent 25 μm. ∗p ≤ 0.05 versus 0’. hBJFs, human BJ fibroblasts; ND, not detected. See also Figure S4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29478844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-00861_rabbit-polyclonal-sucnr1-gpr91-antibody-31020241617520.jpg)
![Western Blot: SUCNR1/GPR91 Antibody - BSA Free [NBP1-00861] - Succinate Signals via SUCNR1 in Mouse & Human NSCs(A–C) Representative confocal microscopy images of meningeal perivascular areas with transplanted fGFP+ iNSCs (A) & NSCs (B) expressing SUCNR1 in the brain of a mouse with EAE. The image in (C) shows transplanted SUCNR1+ iNSCs in close vicinity to SUCNR1+/F4/80+ MPs. Nuclei are stained with DAPI.(D) SUCNR1 protein expression relative to beta -tubulin in vitro. Data are shown as mean (±SEM) of n ≥ 3 independent replicates per condition.(E) Experimental setup for succinate treatment of iNSCs/NSCs in vitro.(F) Intracellular Ca2+ response after treatment with 500 μM succinate (live staining with Fluo-4AM). Representative images (baseline & during stimulation) are pseudocolored with red/blue according to high/low fluorescence intensity. Data are mean changes in fluorescence intensity as delta F/F0 (±SEM) from n ≥ 3 experiments.(G) Phospho-p38 MAPK (P-p38) & total p38 MAPK (p38) protein expression after succinate treatment. Data are P-p38/p38 expression relative to beta -tubulin & expressed as mean fold change (±SEM) versus untreated cells over n ≥ 3 independent experiments per condition.(H) qRT-PCR of SUCNR1 basal expression in human cells. Data are normalized on 18S & expressed as mean fold change (±SEM) versus NSCs from n ≥ 3 independent replicates per condition.(I) Representative blot of SUNCR1 basal protein expression in human cells.(J) P-p38 & p38 protein expression after stimulation with succinate ± pre-treatment with the irreversible inhibitor of the human SUCNR1 4c.The scale bars represent 25 μm. ∗p ≤ 0.05 versus 0’. hBJFs, human BJ fibroblasts; ND, not detected. See also Figure S4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29478844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-00861_rabbit-polyclonal-sucnr1-gpr91-antibody-310202416163741.jpg)
![Western Blot: SUCNR1/GPR91 Antibody [NBP3-27860] - Western blot analysis of lysates from HUVEC and MCF-7 cells, using NBP3-27860. The lane on the right is blocked with the synthesized peptide.](http://images.novusbio.com/fullsize/antibody/nbp3-27860_rabbit-sucnr1-gpr91-pab-western-blot-7520241214..jpg "Western Blot: SUCNR1/GPR91 Antibody [NBP3-27860] - Western blot analysis of lysates from HUVEC and MCF-7 cells, using NBP3-27860. The lane on the right is blocked with the synthesized peptide.")
![Western Blot: SUCNR1/GPR91 Antibody [NBP2-82350] - Host: Rabbit. Target Name: SUCNR1. Sample Type: Fetal Heart lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP2-82350-img0001.jpg "Western Blot: SUCNR1/GPR91 Antibody [NBP2-82350] - Host: Rabbit. Target Name: SUCNR1. Sample Type: Fetal Heart lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: SUCNR1/GPR91 Antibody [NBP2-82350] - Host: Rabbit. Target Name: SUCNR1. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 0.5ug/ml](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Western-Blot-NBP2-82350-img0002.jpg)
![Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NLS3315] - Analysis of anti-GPR91 antibody with human kidney, medulla.](http://images.novusbio.com/fullsize/SUCNR1-GPR91-Antibody-Immunohistochemistry-Paraffin-NLS3315-img0002.jpg "Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NLS3315] - Analysis of anti-GPR91 antibody with human kidney, medulla.")
![Western Blot: GPR91 Overexpression Lysate (Adult Normal) [NBL1-16595] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR91.](http://images.novusbio.com/fullsize/GPR91-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16595-img0002.jpg "Western Blot: GPR91 Overexpression Lysate (Adult Normal) [NBL1-16595] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GPR91.")