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![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.")
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/fullsize/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/fullsize/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NB120-19481-img0001.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 and si-NC into cells for 48 h. Values represent mean +- SEM from four separate experiments. *P less than 0.05, **P less than 0.01, and ***P less than 0.001. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fphys.2018.01270/full) licensed under a CC-BY license.")
![Western Blot: PPAR gamma/NR1C3 Antibody [NB120-19481] - Dnmt3a1 decreases lipid droplet accumulation in the early stage of adipocyte differentiation. (A) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with pSDS-Dnmt3a1 & pSDS-NC for 48 h are showed; scale bar: 25 μm. (B) Lipid droplet content by oil red O staining & extraction method of cells transfected with pSDS-Dnmt3a1 & pSDS-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗∗P < 0.01. (C) Representative images of oil red O staining (red) & DAPI staining (blue) after transfection with si-Dnmt3a1 & si-NC for 48 h are showed; scale bar: 25 μm. (D) Lipid droplet content by oil red O staining & the extraction method of cells transfected with si-Dnmt3a1 & si-NC for 48 h. Values represent mean ± SEM from three independent experiments. ∗P < 0.05. (E) Relative mRNA level of some general genes implicated in adipogenesis or energy homeostasis induced by pSDS-Dnmt3a1 & pSDS-NC into cells. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (F) Protein expression of some general adipose genes induced by pSDS-Dnmt3a1 & pSDS-NC into cells. (G) Relative mRNA level of some general genes involved in adipogenesis or energy homeostasis after transfection with si-Dnmt3a1 & si-NC into cells for 48 h. Values represent mean ± SEM from four separate experiments. ∗P < 0.05, ∗∗P < 0.01, & ∗∗∗P < 0.001. (H) Protein expression of some general genes involved in adipogenesis at 48 h after transfection by si-Dnmt3a1 & si-NC into cells. The band intensity of Western blotting was obtained by averaging the data from three independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30333755), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb120-19481_rabbit-polyclonal-ppar-gamma-nr1c3-antibody-31020241620517.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Analysis in human cell lines RT-4 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-57833-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Analysis in human cell lines RT-4 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-57833-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-57833] - Staining of human cell line RT4 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57833-img0001.jpg)
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223] - The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-59223-img0002.jpg "Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-59223] - The antibody was diluted 1:2,000 in TBS-Tween containing 3% skimmed milk. Figure shows the result of 293T cells transfected with pNTAP-PPARG (lane 1) and of non-transfected cells (lane 2). The position of the protein of interest is indicated on the right the marker (kDa) is shown on the left.")
![ELISA: PPAR gamma/NR1C3 Antibody [NBP2-59223] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human PPARG. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:70,250](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-ELISA-NBP2-59223-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-56194] - Staining of human cell line PC-3 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PPAR-gamma-NR1C3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56194-img0001.jpg "Immunocytochemistry/Immunofluorescence: PPAR gamma/NR1C3 Antibody [NBP2-56194] - Staining of human cell line PC-3 shows localization to nucleus. Antibody staining is shown in green.")
![Western Blot: PPAR gamma Overexpression Lysate (Adult Normal) [NBL1-14633] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR gamma.](http://images.novusbio.com/fullsize/PPAR-gamma-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14633-img0002.jpg "Western Blot: PPAR gamma Overexpression Lysate (Adult Normal) [NBL1-14633] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR gamma.")
![Western Blot: PPAR gamma Overexpression Lysate (Adult Normal) [NBL1-14634] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR gamma.](http://images.novusbio.com/fullsize/PPAR-gamma-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14634-img0002.jpg "Western Blot: PPAR gamma Overexpression Lysate (Adult Normal) [NBL1-14634] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR gamma.")
![Western Blot: PPAR gamma Overexpression Lysate (Adult Normal) [NBP2-05643] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR gamma.](http://images.novusbio.com/fullsize/PPAR-gamma-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05643-img0001.jpg "Western Blot: PPAR gamma Overexpression Lysate (Adult Normal) [NBP2-05643] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAR gamma.")