| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2938-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7047-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71387 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03840-20ul | |||
![Western Blot: FADD Antibody (3X9R6) [NBP3-15951] - Western blot analysis of extracts of various cell lines, using FADD Rabbit mAb (NBP3-15951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FADD-Antibody-3X9R6-Western-Blot-NBP3-15951-img0003.jpg "Western Blot: FADD Antibody (3X9R6) [NBP3-15951] - Western blot analysis of extracts of various cell lines, using FADD Rabbit mAb (NBP3-15951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: FADD Antibody (3X9R6) [NBP3-15951] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FADD Rabbit mAb (NBP3-15951) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FADD-Antibody-3X9R6-Immunohistochemistry-Paraffin-NBP3-15951-img0002.jpg)
![Immunohistochemistry-Paraffin: FADD Antibody (3X9R6) [NBP3-15951] - Immunohistochemistry of paraffin-embedded rat spleen using FADD Rabbit mAb (NBP3-15951) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FADD-Antibody-3X9R6-Immunohistochemistry-Paraffin-NBP3-15951-img0001.jpg)
![Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded human lung using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15951_rabbit-fadd-mab-10l6m0-52202517273575.jpg)
![Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded rat lung using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15951_rabbit-fadd-mab-10l6m0-52202517162379.jpg)
![Western Blot: FADD Antibody (10L6M0) [FADD] - Western blot analysis of various lysates using FADD Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-15951_rabbit-fadd-mab-10l6m0-52202517251920.jpg)
![Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded human colon using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15951_rabbit-fadd-mab-10l6m0-52202517273537.jpg)
![Western Blot: FADD Antibody (10L6M0) [FADD] - Western blot analysis of various lysates using FADD Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15951_rabbit-fadd-mab-10l6m0-52202517202990.jpg)
![Immunohistochemistry: FADD Antibody (10L6M0) [FADD] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using FADD Rabbit mAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-15951_rabbit-fadd-mab-10l6m0-5220251719290.jpg)
![Western Blot: FADD Antibody [NBP1-81831] - Analysis in human cell lines A-431 and HeLa using Anti-FADD antibody. Corresponding FADD RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/FADD-Antibody-Western-Blot-NBP1-81831-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: FADD Antibody [NBP1-81831] - Analysis in human cell lines A-431 and HeLa using Anti-FADD antibody. Corresponding FADD RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FADD Antibody [NBP1-81831] - Staining of human cell line A-431 shows localization to nucleoplasm, cytosol & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FADD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81831-img0010.jpg)
![Immunohistochemistry-Paraffin: FADD Antibody [NBP1-81831] - Staining of human gallbladder shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FADD-Antibody-Immunohistochemistry-Paraffin-NBP1-81831-img0011.jpg)
![Western Blot: FADD Antibody (JE45-25) [NBP3-32328] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32328) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 ug/Lane Lane 2: K-562 cell lysate, 10 ug/Lane Lane 3: Jurkat cell lysate, 10 ug/Lane Lane 4: MCF7 cell lysate, 10 ug/Lane Lane 5: HeLa cell lysate, 10 ug/Lane Lane 6: SK-Br-3 cell lysate, 10 ug/Lane Lane 7: SW480 cell lysate, 10 ug/Lane Lane 8: THP-1 cell lysate, 10 ug/Lane Lane 9: A549 cell lysate, 10 ug/Lane Lane 10: HepG2 cell lysate, 10 ug/Lane Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32328) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32328_rabbit-fadd-mab-je45-25-278202416331258.jpg "Western Blot: FADD Antibody (JE45-25) [NBP3-32328] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32328) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 ug/Lane Lane 2: K-562 cell lysate, 10 ug/Lane Lane 3: Jurkat cell lysate, 10 ug/Lane Lane 4: MCF7 cell lysate, 10 ug/Lane Lane 5: HeLa cell lysate, 10 ug/Lane Lane 6: SK-Br-3 cell lysate, 10 ug/Lane Lane 7: SW480 cell lysate, 10 ug/Lane Lane 8: THP-1 cell lysate, 10 ug/Lane Lane 9: A549 cell lysate, 10 ug/Lane Lane 10: HepG2 cell lysate, 10 ug/Lane Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 90 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32328) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FADD Antibody (JE45-25) [NBP3-32328] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody (NBP3-32328) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32328) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32328_rabbit-fadd-mab-je45-25-298202413585545.jpg)
![Immunocytochemistry/ Immunofluorescence: FADD Antibody (JE45-25) [NBP3-32328] - Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody (NBP3-32328) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (NBP3-32328) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32328_rabbit-fadd-mab-je45-25-29202410484813.jpg)
![Western Blot: FADD Antibody (J1D2) [NBP1-04288] - Cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/FADD-Antibody-J1D2-Western-Blot-NBP1-04288-img0001.jpg "Western Blot: FADD Antibody (J1D2) [NBP1-04288] - Cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunohistochemistry-Paraffin: FADD Antibody (J1D2) [NBP1-04288] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).](http://images.novusbio.com/fullsize/FADD-Antibody-J1D2-Immunohistochemistry-Paraffin-NBP1-04288-img0002.jpg)
![Western Blot: FADD Antibody (SR1465) [NBP3-21838] - Western blot analysis using NBP3-21838 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21838_rabbit-fadd-mab-sr1465-57202316494266.jpg "Western Blot: FADD Antibody (SR1465) [NBP3-21838] - Western blot analysis using NBP3-21838 in A431 cell lysate.")
![Western Blot: FADD Antibody (PSH01-13) [NBP3-32329] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32329) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: HepG2 cell lysate Lane 6: Jurkat cell lysate Lane 7: SK-Br-3 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 28 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32329) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32329_rabbit-fadd-mab-psh01-13-278202416331250.jpg "Western Blot: FADD Antibody (PSH01-13) [NBP3-32329] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32329) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: MCF7 cell lysate Lane 4: A549 cell lysate Lane 5: HepG2 cell lysate Lane 6: Jurkat cell lysate Lane 7: SK-Br-3 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 23 kDa Observed band size: 28 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32329) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: FADD Antibody (PSH01-13) [NBP3-32329] - Immunocytochemistry analysis of A431 cells labeling FADD with Rabbit anti-FADD antibody (NBP3-32329) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (NBP3-32329) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32329_rabbit-fadd-mab-psh01-13-29202410484814.jpg)
![Flow Cytometry: FADD Antibody (PSH01-13) [NBP3-32329] - Flow cytometric analysis of A431 cells labeling FADD. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32329, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32329_rabbit-fadd-mab-psh01-13-29202416453276.jpg)
![Western Blot: FADD Antibody (1C11) [NBP2-02784] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FADD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FADD.](http://images.novusbio.com/fullsize/FADD-Antibody-1C11-Western-Blot-NBP2-02784-img0002.jpg "Western Blot: FADD Antibody (1C11) [NBP2-02784] HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FADD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FADD.")
![Flow Cytometry: FADD Antibody (1C11) [NBP2-02784] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FADD antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/FADD-Antibody-1C11-Flow-Cytometry-NBP2-02784-img0001.jpg)
![Western Blot: FADD Antibody [NBP3-03840] - Analysis of extracts from normal (control) and FADD knockout (KO) HeLa cells, using FADD antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/FADD-Antibody-Knockout-Validated-NBP3-03840-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FADD Antibody [NBP3-03840] - Analysis of extracts from normal (control) and FADD knockout (KO) HeLa cells, using FADD antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: FADD Antibody (JE32-76) [NBP3-32327] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32327) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (10 ug/Lane) Lane 2: C6 cell lysate (10 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32327) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32327_rabbit-fadd-mab-je32-76-278202415295569.jpg "Western Blot: FADD Antibody (JE32-76) [NBP3-32327] - Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (NBP3-32327) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate (10 ug/Lane) Lane 2: C6 cell lysate (10 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Predicted band size: 23 kDa Observed band size: 27 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32327) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: FADD Antibody (1A11) [NBP1-28589] - Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.](http://images.novusbio.com/fullsize/FADD-Antibody-1A11-Western-Blot-NBP1-28589-img0001.jpg "Western Blot: FADD Antibody (1A11) [NBP1-28589] - Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human FADD-UNLB Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.")