![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0006.jpg "In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and counterstained with VECTASHIELD with DAPI.")
| Applications | Func, ICC/IF, IHC, MS |
| Description | VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes. Features: The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4C, protected from light. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 degrees C, protected from light. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Both the VECTASHIELD HardSet and the original VECTASHIELD Mounting Media are available with or without the counterstain DAPI (4,6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm. |
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| Application Notes | The refractive index for VECTASHIELD Mounting Medium is 1.45. Use in IHC-Fr reported in scientific publication (PMID: 32452384).. |
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| Storage | Store at 4C in the dark. |
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reviewed by:
Verified Customer |
11/06/2018 |
Summary
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![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0007.jpg "In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red Substrate. Mounted in VECTASHIELD Mounting Media with DAPI.")
![In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.](http://images.novusbio.com/fullsize/VECTASHIELD-R-Antifade-Mounting-Medium-with-DAPI-In-situ-Hybridization-H-1200-NB-img0008.jpg "In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.")
