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V5 Epitope Tag Antibody [DyLight 650]

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Product Details

Summary
Reactivity ETSpecies Glossary
Applications ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Chicken
Conjugate
DyLight 650

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V5 Epitope Tag Antibody [DyLight 650] Summary

Immunogen
Chickens were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for V5 Epitope Tag Antibody [DyLight 650]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional V5 Epitope Tag Products

Array NB600-379C

Research Areas for V5 Epitope Tag Antibody (NB600-379C)

Find related products by research area.

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

mFluor Violet Conjugated Antibodies

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