| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, IP, ICC/IF |
| Clone | E10/V4RR |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | CoraFluor 1 |
| Description | CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays. |
| Immunogen | GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH |
| Specificity | This product recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. It has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
| Storage | Store at 4C in the dark. Do not freeze. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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V5 Tags are preferable for detection of Exogenous Proteins in Vivo Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of... Read full blog post. |
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Working with the V5 Tag Antibody The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit... Read full blog post. |
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