![Western Blot: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody [GT1071] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Western-Blot-NBP2-43626-img0012.jpg "Western Blot: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 tag antibody [GT1071] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clone | 1071 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | The immunogen used to generate this antibody corresponds to V5 Epitope Tag |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for V5 Epitope Tag Antibody (NBP2-43626)Find related products by research area.
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V5 Tags are preferable for detection of Exogenous Proteins in Vivo Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of... Read full blog post. |
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Working with the V5 Tag Antibody The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Immunocytochemistry-Immunofluorescence-NBP2-43626-img0010.jpg "Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody (1071) [NBP2-43626] - V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: V5 tag stained by V5 tag antibody [1071] diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Analysis of 400 ug cell lysate/extract of V5-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of V5-fused protein by 2 ug of V5 tag antibody [1071] 12% SDS-PAGE The immunoprecipitated V5-fused protein was detected by V5 tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-1071-Immunoprecipitation-NBP2-43626-img0002.jpg "Immunoprecipitation: V5 Epitope Tag Antibody (1071) [NBP2-43626] - Analysis of 400 ug cell lysate/extract of V5-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 ug of preimmune mouse IgG C. Immunoprecipitation of V5-fused protein by 2 ug of V5 tag antibody [1071] 12% SDS-PAGE The immunoprecipitated V5-fused protein was detected by V5 tag antibody diluted at 1:1000. EasyBlot anti-mouse IgG was used as a secondary reagent.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")