![Immunohistochemistry: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of paraffin-embedded cerebellum tissues using TRBC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-1042024915834.jpg%3Fv=1666706090 "Immunohistochemistry: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of paraffin-embedded cerebellum tissues using TRBC1 mouse mAb with DAB staining.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 5D9F1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human TRBC1 (AA: 1-149) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TRBC1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 19.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for TRBC1 Antibody (NBP3-27180)Find related products by research area.
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| Gene Symbol | TRBC1 |
![Immunocytochemistry/Immunofluorescence: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of Hela cells using TRBC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-104202492022.jpg%3Fv=1666706090 "Immunocytochemistry/Immunofluorescence: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of Hela cells using TRBC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Western Blot: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis using TRBC1 mouse mAb against HUVEC (1), Jurkat (2), Hela (3), HUVE-12 (4), A549 (5), C6 (6), Raji (7), and T47D (8) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-10420249141934.jpg%3Fv=1666706089 "Western Blot: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis using TRBC1 mouse mAb against HUVEC (1), Jurkat (2), Hela (3), HUVE-12 (4), A549 (5), C6 (6), Raji (7), and T47D (8) cell lysate.")
![Flow Cytometry: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of Jurkat cells using TRBC1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-10420249141914.jpg%3Fv=1666706089 "Flow Cytometry: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of Jurkat cells using TRBC1 mouse mAb (green) and negative control (red).")
![Western Blot: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-1042024921156.jpg%3Fv=1666706089 "Western Blot: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)")
![Western Blot: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-1042024915826.jpg%3Fv=1666706089 "Western Blot: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.")
![Immunohistochemistry: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of paraffin-embedded testis tissues using TRBC1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-1042024915846.jpg%3Fv=1666706090 "Immunohistochemistry: TRBC1 Antibody (5D9F1) [NBP3-27180] - Analysis of paraffin-embedded testis tissues using TRBC1 mouse mAb with DAB staining.")
![ELISA: TRBC1 Antibody (5D9F1) [NBP3-27180] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27180_mouse-trbc1-mab-5d9f1-bsa-free-1042024916226.jpg%3Fv=1666706089 "ELISA: TRBC1 Antibody (5D9F1) [NBP3-27180] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")