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Total and Free Cholesterol Assay Kit (Fluorometric)

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Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Standard Curve Reference
Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Assay Example
Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma, animal tissue and cell samples
Standard Curve Range
0.12 - 30 umol/L
Sensitivity
0.12 umol/L

Order Details

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Total and Free Cholesterol Assay Kit (Fluorometric) Summary

Description
Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/590 nm)
Assay Time: 20 min
Standard Curve Range
0.12 - 30 umol/L
Sensitivity
0.12 umol/L
Inter-Assay
%CV < 7.3
Intra-Assay
%CV < 1.7
Spike Recovery
Average recovery rate: 96%
Kit Type
Assay Kit (Fluorometric)

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 5.17 mmol/L Cholesterol Standard Solution
  2. Bulfer Solution
  3. Enzyme Reagent 1
  4. Enzyme Reagent 2
  5. Extracting Solution
  6. Substrate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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