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Tenascin C Antibody (T2H5) - Azide and BSA Free

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Immunohistochemistry-Paraffin: Tenascin C Antibody (T2H5) - Azide and BSA Free [NBP2-47919] - Human lung carcinoma stained with Tenascin C Monoclonal Antibody (T2H5) at 4 ug/ml. Antigen retrieval in 10mM Tris with 1mM ...read more

Product Details

Summary
Reactivity Hu, Rt(-)Species Glossary
Applications IHC
Clone
T2H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

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Tenascin C Antibody (T2H5) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44582).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Human breast carcinoma (Uniprot: P24821)
Localization
Connective tissue matrix
Marker
Stromal Marker For Epithelial Malignancy
Specificity
In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this monoclonal antibody is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Formalin-fixed): 2-4ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reactivity Notes

Does not react with Rat.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Tenascin C Antibody (T2H5) - Azide and BSA Free

  • 150-225
  • Cytotactin
  • Glioma-associated-extracellular matrix antigen
  • GMEM
  • GP 150-225
  • hexabrachion (tenascin C, cytotactin)
  • hexabrachion (tenascin)
  • Hexabrachion
  • HXB
  • HXBcytotactin
  • JI
  • MGC167029
  • Myotendinous antigen
  • neuronectin
  • Tenascin C
  • Tenascin J1
  • tenascin
  • tenascin-C isoform 14/AD1/16
  • Tenascin-C
  • TNC
  • TN-C
  • TNGP

Background

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Tenascin C Antibody (NBP2-47919). (Showing 1 - 1 of 1 FAQ).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

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Bioinformatics

Gene Symbol TNC