Streptavidin [Cy3] Summary
| Description |
Features:
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin.
Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
Excitation: 552 nm
Emission: 565 nm
Color: Red
|
Applications/Dilutions
| Dilutions |
- Flow Cytometry
- Immunofluorescence
- In-situ Hybridization
|
| Application Notes |
Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. . |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
10 mM HEPES, 0.15 M NaCl, pH 7.5 |
| Preservative |
0.08% Sodium Azide |
| Concentration |
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Alternate Names for Streptavidin [Cy3]
Background
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin. These fluorescent conjugates can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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