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Recombinant Streptavidin His Protein

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SDS-Page: Recombinant Bacterial Streptavidin Protein [NBP2-22641]

Product Details

Summary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

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Recombinant Streptavidin His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 37-159 of Streptomyces Streptavidin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Streptomyces avidinii

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Streptavidin His Protein

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2
  • Streptavidin

Background

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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