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Streptavidin Native Protein

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Flow Cytometry: Streptavidin Native Protein [NBP2-29360] - Streptavidin Full length Protein [NBP2-29360] - Staining of C57BL/6 splenocytes with Anti-Mouse CD3e Biotin (cat. NBP2-29360), followed by 0.06 ug of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Conjugate
PE/Cy7
Concentration
0.2 mg/ml

Order Details

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Streptavidin Native Protein Summary

Description
A full-length protein to Streptavidin.

Source: Streptomyces avidinii

This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Source
Streptomyces avidinii
Protein/Peptide Type
Native Protein
Purity
>90% pure
Endotoxin Note
Not Tested

Applications/Dilutions

Dilutions
  • Flow Cytometry
Application Notes
Streptavidin PE-Cyanine7 has been tested by flow cytometric analysis to detect biotinylated primary antibodies. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically, but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
>90% pure

Alternate Names for Streptavidin Native Protein

  • SA V1; SA V2; Streptavidin V1; Streptavidin V2
  • Streptavidin

Background

The streptavidin fluorochrome conjugates are commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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