Human Spleen Membrane Tissue Lysate (Adult Membrane Normal) Summary
| Description |
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity |
Membrane Protein - Human Adult Normal Tissue: Spleen |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration |
2.0 mg/ml |
Lysate Details for Array
| Type |
Tissue |
| Life Stage |
Adult |
| Tissue |
Blood Lymph |
| Protein State |
Native |
| Tissue Condition |
Normal |
| Subcellular Fraction |
Membrane |
Background
All the protein lysates were prepared from liquid nitrogen fresh frozen tissues.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Spleen Lysate (NB820-60617). (Showing 1 - 1 of 1 FAQ).
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What is the source of the Human Spleen Membrane Tissue Lysate (Adult Membrane Normal) NB820-60617? Specifically, where are patients generally located/tissues acquired, how are lots divided, and how is the tissue harvested and fractionated?
- The source of this tissue is an autopsy. The current lot is derived from a single extraction of the tissue (additional extractions can be procured but there would be additional fees). The protein lysate was prepared from liquid nitrogen fresh frozen tissues. Tissue membrane protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. The protein is stored in a buffer with protease inhibitor cocktail.
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