Red Blood Cell (RBC) Lysis Buffer [NBP2-29442] - data shows before (left) and after (right) human whole blood cells were lysed using the Red Blood Cell (RBC) lysis buffer. Image from verified customer review.
The Red Blood Cell (RBC) Lysis Buffer is for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic issues such as spleen and human peripheral blood. This buffer contains ammonium chloride, which lyses red cells with minimal effect on lymphocytes. Nucleated red blood cells are not effectively lysed with ammonium chloride. RBC lysis is not necessary when working with mouse thymus and lymph node.
Applications/Dilutions
Dilutions
Functional reported in scientific literature (PMID 24371146)
Reviewed Applications
Read 1 Review rated 5 using NBP2-29442 in the following application:
Mouse reactivity reported in scientific literature (PMID: 24371146). Human reactivity reported in scientific literature (PMID: 24080766)
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
Dilute 10X RBC Lysis Buffer to 1X in deionized water before use (1 ml of 10X RBC lysis buffer with 9 ml of deionized water). Bring to room temperature prior to use.
Alternate Names for Red Blood Cell (RBC) Lysis Buffer
RBC
Red Blood Cell (RBC) Lysis Buffer (10X)
Red Blood Cell (RBC) Lysis Buffer
Red Blood Cell (RBC) Lysis
Red Blood Cell (RBC)
Red Blood Cell
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for Red Blood Cell (RBC) Lysis Buffer (NBP2-29442)(5)
We have publications tested in 3 confirmed species: Human, Mouse, Rat.
We have publications tested in 3 applications: FLOW, Func, Red Blood Cell Lysis.
![Red Blood Cell (RBC) Lysis Buffer [NBP2-29442] - data shows before (left) and after (right) human whole blood cells were lysed using the Red Blood Cell (RBC) lysis buffer. Image from verified customer review.](http://images.novusbio.com/fullsize/Red-Blood-Cell-RBC-Lysis-Buffer-NBP2-29442-img0002.jpg "Red Blood Cell (RBC) Lysis Buffer [NBP2-29442] - data shows before (left) and after (right) human whole blood cells were lysed using the Red Blood Cell (RBC) lysis buffer. Image from verified customer review.")
