2 μg/lane of Recombinant SARS-CoV-2 Membrane Fc Chimera Protein (Catalog # 10690-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions. Visualized by Coomassie® Blue staining, showing ...read more
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
33-43 kDa, under reducing conditions
Publications
Read Publication using 10690-CV in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant SARS-CoV-2 Membrane Fc Chimera Protein, CF
M protein
Membrane
Background
SARS-CoV-2, which causes the global pandemic
coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as
coronaviruses that are commonly comprised of four structural proteins: Spike
protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid
protein (N) (1). M protein is the most abundant structural protein in the
coronavirus membrane, and it is predicted to span the membrane three times,
with a short N-terminal domain outside the viral envelope and a long C-terminal
domain inside the virion (2). SARS-CoV-2 M protein is a 222 amino acid (aa)
glycoprotein that is composed of an 18 aa N-terminal domain on the viral
surface, 3 transmembrane domains, and a 122 aa C-terminal domain
inside the viral envelope. SARS-CoV-2 M
protein shares 89.14%, 98.6%, 98.2%, and 38.36% aa similarity with SARS-CoV-1,
bat SARS-CoV, pangolin SARS-CoV, and MERS-CoV M proteins, respectively (3). The
M protein of coronavirus plays an important role in assembly of viral particles
by interacting with other structural proteins, especially with the E protein (4,
5). In SARS-CoV-2, M protein, combined with E protein, regulates intracellular
trafficking of the S Protein and its unique furin-mediated processing (6).
Wu, F. et al. (2020) Nature 579:265.
Mousavizadeh, L. and S. Ghasemi (2020) J. Microbiol. Immunol. Infect. doi:10.1016/j.jmii.2020.03.022.
Thomas, S. (2020) Pathog. Immun. 5:342.
Masters, P.S. (2006) Adv. Virus. Res. 66:193.
Corse, E. and C.E. Machamer (2003) Virology 312:25.
Boson, B. et al. (2020) bioRxiv doi:10.1101/2020.08.24.260901.
Publications for Membrane (10690-CV)(1)
We have publications tested in 1 confirmed species: N/A.
We have publications tested in 1 application: Bioassay.
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