Recombinant Mouse IGFBP-4 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

IGFBP‑4 (insulin‑like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins that maintain IGF‑I and IGF‑II in the circulation and direct them to their target tissues (1, 2). Mouse IGFBP‑4 cDNA encodes 254 amino acids (aa) that include a 21 aa signal peptide and a 233 aa cysteine-rich mature protein that contains an N‑terminal IGFBP domain, a linker sequence, and a C‑terminal thyroglobulin domain. Mature mouse IGFBP‑4 shares 99% aa sequence identity with rat IGFBP‑4, and 90‑91% with human, canine, bovine, ovine and porcine IGFBP‑4. Both domains interact with IGFs, although the N‑terminal domain is primary (2). The linker contains a cleavage site that is accessible by PAPP‑A (pregnancy-associated plasma protein A) when IGFs are bound (2, 3). IGFBP‑4 fragments detected in the plasma of pregnant women have lowered affinity for IGFs, thus up‑regulating IGF bioavailability for receptor binding (2). Unlike other family members, IGFBP‑4 does not interact with the extracellular matrix or translocate to the nucleus (2). Like other family members, it is expressed early in development and has both IGF‑dependent and ‑independent activities (2, 4). IGFBP‑4 expression in mesenchymal stem cells is up‑regulated by the cardiac transcription factor GATA‑4 (5). It then enhances cardiomyocyte differentiation by interacting with Frizzled and LRP molecules, inhibiting canonical Wnt signaling (6, 7). IGF can sequester IGFBP‑4 when present, inhibiting its ability to support cardiomyocyte differentiation (3, 4). IGFBP‑4 is also up‑regulated in osteoblasts in response to estrogen (8). Intact or proteolytically cleaved IGFBP‑4 may either promote or inhibit cancer cell growth, motility and invasion depending on cell type, tissue and stage of the cancer (9‑11). It is shown to inhibit IGF‑1‑ or FGF‑2‑induced angiogenesis, but not that induced by VEGF (10).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.

Showing Publication 1 - 1 of 1.

Publication using 8066-GB/CF	Applications	Species
MW Laschke, E Kontaxi, C Scheuer, A He beta , P Karschnia, MD Menger Insulin-like growth factor 1 stimulates the angiogenic activity of adipose tissue-derived microvascular fragments J Tissue Eng, 2019-09-27;10(0):2041731419879. 2019-09-27 [PMID: 31632630] (Bioassay, Mouse)	Bioassay	Mouse

Array 8066-GB/CF

• IGFBP-4 Antibodies
• IGFBP-4 ELISA Kits
• IGFBP-4 Lysate
• IGFBP-4 Proteins
• IGFBP-4 ELISA
• IGFBP-4 Proteins and Enzymes