Recombinant Monkeypox Virus A35R His-tag Protein, CF Summary
| Details of Functionality |
Bioassay data are not available. |
| Source |
Human embryonic kidney cell, HEK293-derived monkeypox virus zaire-96-i-16 A35R protein
Arg58-Thr181, with a C-terminal 6-His tag |
| Accession # |
|
| N-terminal Sequence |
Arg58 |
| Protein/Peptide Type |
Recombinant Proteins |
| Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE |
13-21 kDa, under reducing conditions. |
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Monkeypox Virus A35R His-tag Protein, CF
Background
Monkeypox Virus (MPXV), the virus that causes monkeypox infection
in both humans and animals, is a double-stranded DNA virus that has had a
recent global outbreak in 2022 (1). MPXV belongs to the Poxviridae family of
viruses (2). It consists of several key subunits including a surface membrane
fusion protein (A29L, ~14 kDa), two separate envelope proteins (A30L ,~14 kDa
and H3L, ~32kDa), an envelope glycoprotein (A35R ~15 kDa), a receptor
glycoprotein that mimics IFN‑alpha/beta (B16, ~37kDa), a palmitoylated EEV
membrane glycoprotein (C19L, ~35 kDa), a secreted IL-18 binding protein (D6L,
~14kDa), a cell surface-binding protein (E8L, ~32 kDa), a telomere binding
protein (I1L, ~36kDa), and a subunit required for DNA packaging (L1R, 18 kDa)
(2-3). The envelope glycoprotein A35R is highly conserved among poxviruses. It is not required for replication, but it was reported to play a role in virulence (4). In addition, A35R was showed to inhibit MHC class II antigen presentation (5).
- Breman, J.G. et al. (1980) Bull World Health Organ. 58:165.
- Farahat, R.A. et al. (2022) Infez Med. 30:372.
- Schelkunov, S.N. et al. (2002) Virology 297:172.
- Roper, R. L. (2006) J. Virol. 80:306.
- Rehm, K. E. et al. (2010) Virology 397:176.
Publications for Monkeypox Virus A35R (11285-MX) (0)
There are no publications for Monkeypox Virus A35R (11285-MX).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Monkeypox Virus A35R (11285-MX) (0)
There are no reviews for Monkeypox Virus A35R (11285-MX).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for Monkeypox Virus A35R (11285-MX) (0)
Additional Monkeypox Virus A35R Products
Blogs on Monkeypox Virus A35R
