Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF Summary
| Details of Functionality |
Measured by its binding ability in a functional ELISA with Recombinant
Human DPPIV/CD26 (High Purity Dimer) (Catalog #
9168-SE). |
| Source |
Human embryonic kidney cell, HEK293-derived mers-cov Spike protein MERS-CoV Spike
(Tyr18-Lys1294)(Arg748Ser, Arg751Ser, Val1060Pro, Leu1061Pro)
Accession # YP_007188579.1 | GCN4-IZ | 6-His tag | | N-terminus | | C-terminus | |
|
| Accession # |
|
| N-terminal Sequence |
Tyr18 |
| Protein/Peptide Type |
Recombinant Proteins |
| Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE |
145-175 kDa, under reducing conditions |
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant MERS-CoV Spike (GCN4-IZ) His-tag Protein, CF
Background
MERS-CoV (also known as HCoV-EMC), which causes the Middle
East Respiratory Syndrome (MERS), belongs to a family of viruses known as
coronaviruses that also include SARS-CoV-1 and SARS-CoV-2. Coronaviruses are
commonly comprised of a large plus-strand RNA genome and four structural
proteins: Spike protein (S), Envelope protein (E), Membrane protein (M) and
Nucleocapsid protein (N) (1). MERS-CoV Spike Protein (S Protein) is a
glycoprotein that mediates membrane fusion and viral entry, and it consists of
two subunits, S1 and S2. The S1 subunit is focused on attachment of the protein
to the host receptor while the S2 subunit is involved with cell fusion (3). The
MERS-CoV S protein shares 31% and 29% amino acid (aa) sequence identity with
SARS-CoV S1 subunit and SARS-Cov2 S1 subunit, respectively. The low aa sequence
homology is consistent with the finding that MERS-CoV and SARS-CoV bind
different cellular receptors (4). Unlike SARS-CoV and SARS-CoV2, which engage
ACE-2 as their receptors for cell entry, MERS-CoV employs Dipeptidyl Peptidase 4
(DPP4; also known as CD26) as its functional receptor (4). Based on structural
biology studies, the receptor binding domain (RBD) of MERS-CoV spike protein is
located in the C-terminal region of S1 subunit and consists of a core subdomain
and a receptor-binding subdomain (5, 6). The S1 subunit, especially the RBD region,
was commonly targeted for vaccinations or antiviral therapy against MERS (7-9).
- Bermingham, A. et al. (2012) Euro Surveill. 17:20290.
- Zaki, A.M. et al. (2012) N. Engl. J. Med. 367:1814.
- Li, Y. et al. (2019) Engineering. 5:940.
- Raj, V.S. et al. (2013) Nature 495:251.
- Lu, G. et al. (2013) Nature 500:227.
- Wang, N. et al. (2013) Cell. Res. 23:986.
- Corti, D. et al. (2016) J. Infect. Public Health 9:231.
- Tang, X.C. et al. (2014) Proc. Natl. Acad. Sci. USA 111:E2018.
- Jiang, L. et al. (2014) Sci. Transl. Med. 6:234ra59.
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